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| These knockout mice lack exons 39-40 of the leucine-rich repeat kinase 2 (Lrrk2) gene and may be useful for studying the role of Lrrk2 in the progression of Parkinson's Disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names C57BL/6N-Lrrk2tm1.1Youy/J (Changed: 23-MAY-12 ) Type Coisogenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Heterozygote x +/+ sibling (Female x Male) 30-AUG-11 Species laboratory mouse Generation F?+F3 (16-OCT-12)
Generation DefinitionsDonating Investigator Kuldip Dave, The Michael J. Fox Foundation Description
These mice lack exons 39-40 of the leucine-rich repeat kinase 2 (Lrrk2) gene, abolishing gene function. LRRK2 protein, also known as Dardarin, contains multiple functional domains and may function as both an active GTPase and kinase. LRRK2 may regulate alpha-synuclein-mediated neuropathology through modulation of intracellular trafficking and accumulation of SNCA protein. These mice may be useful for studying the role of Lrrk2 in the progression of Parkinson's Disease.Development
A targeting vector was designed to replace exons 39-40 of the leucine-rich repeat kinase 2 (Lrrk2) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into C57BL/6NTac-derived B6-3 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase expression plasmid to delete the neo cassette. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6NTac mice. Upon arrival, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Parkinson's Disease Models
View Parkinson's Disease Models (109 strains)
016122 C57BL/6N-Lrrk1tm1.1Mjff Lrrk2tm1.1Mjff/J
012453 B6.129X1(FVB)-Lrrk2tm1.1Cai/J 009346 B6.Cg-Lrrk2tm1.1Shn/J 012466 B6.Cg-Tg(Lrrk2)6Yue/J 012467 B6.Cg-Tg(Lrrk2*G2019S)2Yue/J 009347 B6;129-Lrrk2tm1.1Shn/J 016209 B6;129-Lrrk2tm2.1Shn/J 016210 B6;129-Lrrk2tm3.1Shn/J 012444 C57BL/6-Lrrk2tm1Mjfa/J
Additional Web Information
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Parkinson Disease 8, Autosomal Dominant; PARK8 (LRRK2)
assigned by genotype
Lrrk2tm1.1Mjff/Lrrk2tm1.1Mjff
C57BL/6N-Lrrk2tm1.1Mjff/J
- no phenotypic analysis
- *normal* no phenotypic analysis
- no phenotypic analysis reported (MGI Ref ID J:170000)
This mouse can be used to support research in many areas including:
Neurobiology Research
Neurodegeneration
Parkinson's Disease
LRRK2 strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Lrrk2tm1.1Mjff | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, The Michael J Fox Foundation | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | Lrrk2tm1.1Youy; | ||
| Mutation Made By | Yingbin Ouyang, Taconic | ||
| Strain of Origin | C57BL/6NTac | ||
| Gene Symbol and Name | Lrrk2, leucine-rich repeat kinase 2 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | 4921513O20Rik; 9330188B09Rik; AURA17; AW561911; D630001M17Rik; DARDARIN; Gm927; LOC381026; PARK8; RIKEN cDNA 4921513O20 gene; RIKEN cDNA 9330188B09 gene; RIKEN cDNA D630001M17 gene; RIPK7; ROCO2; cDNA sequence, clone 3-75; cI-46; gene model 927, (NCBI); | ||
| General Note | ES cell line = B6-3 (C57BL/6NTac). | ||
| Molecular Note | A targeting vector was designed to replace exons 39-40 of the leucine-rich repeat kinase 2 (Lrrk2) gene with a loxP-flanked neomycin resistance (neo) cassette. Correctly targeted ES cells were transiently transfected with a Cre recombinase expression plasmid to delete the neo cassette. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6NTac mice. [MGI Ref ID J:170000] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Lrrk2tm1.1Mjff, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Lrrk2tm1.1Mjff relatedOuyang Y. 2011. Generation of a new Lrrk2 knockout allele MGI Direct Data Submission :. [MGI Ref ID J:170000]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wild type mice from the colony or to C57BL/6NJ inbred mice (Stock No. 005304). Mating System Heterozygote x +/+ sibling (Female x Male) 30-AUG-11
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Lrrk2tm1.1Mjff
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Lrrk2tm1.1Mjff x Wild-type for Lrrk2tm1.1Mjff $296.00 Wild-type for Lrrk2tm1.1Mjff x Heterozygous for Lrrk2tm1.1Mjff Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Lrrk2tm1.1Mjff
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Lrrk2tm1.1Mjff x Wild-type for Lrrk2tm1.1Mjff $384.80 Wild-type for Lrrk2tm1.1Mjff x Heterozygous for Lrrk2tm1.1Mjff Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 005304 C57BL/6NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
- Notice regarding distribution of this strain.
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Contact information
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| phone: | 207-288-6470 |
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