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| These CCSP-rtTA transgenic mice express the rtTA protein under the control of the rat Scgb1a1 gene promoter and provides an improved "Tet-On" tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 11-DEC-12 Species laboratory mouse Generation F?pN1+F3 (25-FEB-13)
Generation DefinitionsDonating Investigator Dr. Jeffrey Whitsett, Children's Hospital Medical Center Description
These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter. In situ hybridization detects rtTA mRNA in bronchial and type II epithelial cells of lung tissue from adult transgenic mice treated with doxycycline for seven days. Induction of transgene expression is detected as early as postconception day 14 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene can be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice hemizygous for the CCSP-rtTA transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.This founder line has less non-doxycycline-dependent expression of rtTA than Founder Line 1 (See Stock No. 006232, and Stock No. 006242)
Development
A transgenic construct containing 3.7kb sequence of the rat Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and 2.0kb sequence of the human grouth hormone gene, containing introns and polyadenylation site, was injected into mixed C57BL/6 and 129 background embryos. Mice from founder line 2 were bred to FVB/N mice. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Scgb1a1
006438 B6.Cg-Tg(Scgb1a1-Scnn1b)6608Bouc/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 005315 B6;C3H-Tg(Scgb1a1-Scnn1b)6608Bouc/J 006176 B6C3Fe-Tg(Scgb1a1-Scnn1b)6608Bouc/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J View Strains carrying other alleles of Scgb1a1 (6 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
lung
Immunology, Inflammation and Autoimmunity Research
Cystic Fibrosis
Internal/Organ Research
Lung Defects
Research Tools
Cancer Research
Tetop Tet System
Developmental Biology Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Tetop Tet System
Immunology and Inflammation Research
Internal/Organ Research
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Scgb1a1-rtTA)2Jaw | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Jeffrey A Whitsett | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | SP-C-rtTA (Line 2); | ||
| Mutation Made By | Dr. Jeffrey Whitsett, Children's Hospital Medical Center | ||
| Strain of Origin | C57BL/6 and 129 | ||
| Site of Expression | Transgene is expressed in bronchial and type II epithelial cells of lung tissue from adult transgenic mice. Induction of transgene expression is detected as early as postconception day 14. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Scgb1a1, secretoglobin, family 1A, member 1 (uteroglobin), rat | ||
| Molecular Note | The transgenic construct contains 3.7kb sequence of the rat Scgb1a1 (secretoglobin, family 1A, member 1 (uteroglobin) gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and 2.0kb sequence of the human grouth hormone gene, containing introns and polyadenylation site. Line 2 was generated. [MGI Ref ID J:179352] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Scgb1a1-rtTA)1Jaw, Melt Curve Analysis
Tg(tTA), QPCR
Tg(Scgb1a1-rtTA)1Jaw, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Perl AK; Zhang L; Whitsett JA. 2009. Conditional expression of genes in the respiratory epithelium in transgenic mice: cautionary notes and toward building a better mouse trap. Am J Respir Cell Mol Biol 40(1):1-3. [PubMed: 19075182] [MGI Ref ID J:179352]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous or homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 11-DEC-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(Scgb1a1-rtTA)2Jaw
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(Scgb1a1-rtTA)2Jaw x Homozygous for Tg(Scgb1a1-rtTA)2Jaw Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(Scgb1a1-rtTA)2Jaw
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(Scgb1a1-rtTA)2Jaw x Homozygous for Tg(Scgb1a1-rtTA)2Jaw Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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