Strain Name:

STOCK Tg(SFTPC-rtTA)2Jaw/J

Stock Number:

016146

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Availability:

Cryopreserved - Ready for recovery

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These transgenic mice express the rtTA protein under the control of the human SFTPC promoter. This strain provides an improved Tet-On tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating InvestigatorDr. Jeffrey Whitsett,   Children's Hospital Medical Center

Description
These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human SFTPC, surfactant, pulmonary-associated protein C, promoter. In situ hybridization detects rtTA mRNA in lung peripheral epithelial cells from adult mice and 15 postconception day-aged embryos from doxycycline treated dams. Induction of transgene expression is detected as early as postconception day 12.5 when the pregnant female is treated with doxycycline. When mated to a second transgenic strain carrying a gene under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene may be regulated with the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. Mice that are homozygous for this transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. This strain provides a Tet-On tool that allows the inducible expression of genes in the developing and adult lung and respiratory epithelium.

This founder line has less non-doxycycline-dependent expression than Founder Line 5 (Stock No. 006225, Stock No. 006235, and Stock No. 006245)

Development
A transgenic construct containing 3.7kb sequence of the human SFTPC, surfactant, pulmonary-associated protein C, gene promoter, the reverse tetracycline regulatable transactivator protein (rtTA) gene, and SV40 polyadenylation signal sequence was injected into mixed C57BL/6 and 129 background embryos. Mice from founder line 2 were bred to FVB/N mice. Upon arrival at The Jackson Laboratory, these mice were bred with C57BL/6J mice (Stock No. 000664) to establish the colony.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of SFTPC
006235   B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006245   C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006225   FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
View Strains carrying other alleles of SFTPC     (3 strains)

Strains carrying other alleles of rtTA
016567   129S.Cg-Tg(Hoxb7-rtTA*M2)2Cos/J
017983   B6.Cg-Col1a1tm9(tetO-Dnmt3b_i1)Jae Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
014588   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm6(tetO-MSI2)Jae/J
014602   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-mCherry)Eggn/J
023749   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae Tg(tetO-Pou5f1,-Sox2,-Klf4,-Myc)1Srn/J
006965   B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016997   B6.Cg-Tg(Axin2-rtTA2S*M2)7Cos/J
012418   B6.Cg-Tg(CD68-rtTA2S*M2)3Mpil/Mmjax
014098   B6.Cg-Tg(GFAP-rtTA*M2)1Rmra/J
007176   B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J
006235   B6.Cg-Tg(SFTPC-rtTA)5Jaw/J
006232   B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
021025   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
006911   B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J
016836   B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm7(tetO-HIST1H2BJ/GFP)Jae/J
012433   B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J
021187   B6;FVB-Tg(Pbsn-rtTA*M2)42Xy/J
010574   B6;SJL-Tg(Gh1-rtTA)4-3Jek/J
007678   B6;SJL-Tg(KRT14-rtTA)208Jek/J
010576   B6;SJL-Tg(MMTV-rtTA)4-1Jek/J
010549   B6N.Cg-Tg(Prkcd-glc-1-rtTA)2And/J
016532   B6N.FVB(Cg)-Tg(CAG-rtTA3)4288Slowe/J
006245   C.Cg-Tg(SFTPC-rtTA)5Jaw/J
006242   C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
017955   C57BL/6-Tg(Gfap-rtTA,tetO-MAOB,-lacZ)1Jkan/J
008099   FVB-Tg(KRT14-rtTA)F42Efu/J
004127   FVB-Tg(Nes-rtTA)306Rvs/J
008326   FVB-Tg(Pomc-rtTA)1Rck/J
006225   FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J
006222   FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J
008202   FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J
006875   FVB/N-Tg(Tagln-rtTA)E1Jwst/J
004602   NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ
005734   NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ
011004   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011011   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm4(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae/J
011013   STOCK Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm5(tetO-Pou5f1,-Klf4,-Myc)Jae/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
016116   STOCK Waptm2(rtTA)Kuw/J
003273   STOCK Tg(CMV-rtTA)4Bjd/J
018156   STOCK Tg(Drd1a-rtTA)ARgmk/J
019110   STOCK Tg(Hoxb7-rtTA*M2)RS40BCos/Mmjax
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008250   STOCK Tg(Ins2-rtTA)2Efr/J
017519   STOCK Tg(KRT5-rtTA)T2D6Sgkd/J
016145   STOCK Tg(Scgb1a1-rtTA)2Jaw/J
005493   STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J
View Strains carrying other alleles of rtTA     (48 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      lung

Immunology, Inflammation and Autoimmunity Research
Cystic Fibrosis

Internal/Organ Research
Lung Defects

Research Tools
Cancer Research
      Tetop Tet System
Developmental Biology Research
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Tetop Tet System
Immunology, Inflammation and Autoimmunity Research
Internal/Organ Research
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(SFTPC-rtTA)2Jaw
Allele Name transgene insertion 2, Jeffrey A Whitsett
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) SP-C-rtTA (line 2);
Mutation Made ByDr. Jeffrey Whitsett,   Children's Hospital Medical Center
Strain of Origin129 and C57BL/6
Site of ExpressionTransgene is expressed in respiratory epithelial cells.
Expressed Gene rtTA, reverse tetracycline-controlled transactivator, E. coli
The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16).
Promoter SFTPC, surfactant protein C, human
General Note Phenotypic Similarity to Human Syndrome: Bronchopulmonary dysplasia (BPD) (J:171151).
Molecular Note The transgene is composed of 3.7 kb of human SFTPC promoter, a reverse tetracycline responsive transactivator gene (rt-TA), and a 0.45 kb SV40 polyadenylation signal. Transgene expression was confirmed by RT-PCR analysis. The human SFTPC promoter is active in respiratory epithelial cells. [MGI Ref ID J:61736]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tTA), QPCR
Tg(tTA), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tg(SFTPC-rtTA)2Jaw related

Chen S; Rong M; Platteau A; Hehre D; Smith H; Ruiz P; Whitsett J; Bancalari E; Wu S. 2011. CTGF disrupts alveolarization and induces pulmonary hypertension in neonatal mice: implication in the pathogenesis of severe bronchopulmonary dysplasia. Am J Physiol Lung Cell Mol Physiol 300(3):L330-40. [PubMed: 21239535]  [MGI Ref ID J:171151]

Hokuto I; Perl AK; Whitsett JA. 2003. Prenatal, but not postnatal, inhibition of fibroblast growth factor receptor signaling causes emphysema. J Biol Chem 278(1):415-21. [PubMed: 12399466]  [MGI Ref ID J:133366]

Tichelaar JW; Lu W; Whitsett JA. 2000. Conditional expression of fibroblast growth factor-7 in the developing and mature lung. J Biol Chem 275(16):11858-64. [PubMed: 10766812]  [MGI Ref ID J:61736]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, the donating investigator confirms that homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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