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| The mouse Per1 (period homolog 1 (Drosophila)) promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in this transgenic strain. These mice may be useful in studies of circadian and photic regulation of the mammalian circadian oscillator. | |||||||||||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 22-SEP-11 Mating System Hemizygote x Noncarrier (Female x Male) 22-SEP-11 Species laboratory mouse Generation N11+F1 (16-APR-12)
Generation DefinitionsDonating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr Description
In this transgenic strain, the mouse Per1 (period homolog 1 (Drosophila)) promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). Long-term circadian rhythms of luminescence are also seen in SCN slice culture. Pulses of light rapidly induce high luciferase mRNA expression in the SCN that, unlike endogenous Per1 mRNA levels, are sustained and do not return to baseline. This P1L128 line carries 2 copies of the transgene.Development
6.75 kb of the mouse Per1 promoter (including exon 1, intron 1 and the untranslated portion of exon 2 up to the initiaton ATG) drives expression of luciferase in this transgenic strain. The initiator ATG of both Per1 and luciferase were engineered to coincide. The vector was introduced to CD1 embryos. This strain was backcrossed to C57BL/6J for 10 generations by the donating laboratory. This strain carries 2 copies of the transgene.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Per1
010831 129S-Per1tm1Drw/J 010491 B6.129-Per1tm1Drw/J 016187 B6.BTBR-Tg(Per1-luc,Per1)1Jt/J 006851 B6.Cg-Tg(Per1-luc)025Jt/J 016166 B6.Cg-Tg(Per1-luc)141Jt/J View Strains carrying other alleles of Per1 (5 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Circadian Rhythms
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Per1-luc)128Jt | ||
|---|---|---|---|
| Allele Name | transgene insertion 128, Joseph S Takahashi | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Per1 Luc 128; | ||
| Mutation Made By | Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr | ||
| Strain of Origin | CD-1 | ||
| Site of Expression | Per1 promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei. | ||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | Per1, period circadian clock 1, mouse, laboratory | ||
| Molecular Note | 6.75 kb of the mouse Per1 promoter (including exon 1, intron 1 and the untranslated portion of exon 2 up to the initiaton ATG) drives expression of luciferase. [MGI Ref ID J:73700] | ||
| Gene Symbol and Name | Tg(Per1-luc)128Jt, transgene insertion 128, Joseph S Takahashi | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Per1-luc), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Wilsbacher LD; Yamazaki S; Herzog ED; Song EJ; Radcliffe LA; Abe M; Block G; Spitznagel E; Menaker M; Takahashi JS. 2002. Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice invivo. Proc Natl Acad Sci U S A 99(1):489-94. [PubMed: 11752392] [MGI Ref ID J:73700]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred with wildtype mice. Mating System Noncarrier x Hemizygote (Female x Male) 22-SEP-11 Hemizygote x Noncarrier (Female x Male) 22-SEP-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
This strain is currently Under Development for Cryo.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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General Terms and Conditions
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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