|The mouse Per1 (period homolog 1 (Drosophila)) promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in this transgenic strain. These mice may be useful in studies of circadian and photic regulation of the mammalian circadian oscillator.|
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 22-SEP-11 Mating System Hemizygote x Noncarrier (Female x Male) 22-SEP-11 Species laboratory mouse Generation N11+F1 (16-APR-12)
Donating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
In this transgenic strain, the mouse Per1 (period homolog 1 (Drosophila)) promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei (SCN). Long-term circadian rhythms of luminescence are also seen in SCN slice culture. Pulses of light rapidly induce high luciferase mRNA expression in the SCN that, unlike endogenous Per1 mRNA levels, are sustained and do not return to baseline. This P1L128 line carries 2 copies of the transgene.
6.75 kb of the mouse Per1 promoter (including exon 1, intron 1 and the untranslated portion of exon 2 up to the initiaton ATG) drives expression of luciferase in this transgenic strain. The initiator ATG of both Per1 and luciferase were engineered to coincide. The vector was introduced to CD1 embryos. This strain was backcrossed to C57BL/6J for 10 generations by the donating laboratory. This strain carries 2 copies of the transgene.
|Considerations for Choosing Controls|
Strains carrying other alleles of Per1
010831 129S-Per1tm1Drw/J 010491 B6.129-Per1tm1Drw/J 016187 B6.BTBR-Tg(Per1-luc,Per1)1Jt/J 006851 B6.Cg-Tg(Per1-luc)025Jt/J 016166 B6.Cg-Tg(Per1-luc)141Jt/JView Strains carrying other alleles of Per1 (5 strains)Strains carrying other alleles of luc
006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 006852 B6.129S6-Per2tm1Jt/J 016187 B6.BTBR-Tg(Per1-luc,Per1)1Jt/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J 006851 B6.Cg-Tg(Per1-luc)025Jt/J 016166 B6.Cg-Tg(Per1-luc)141Jt/J 005999 B6.Cg-Tg(SBE/TK-luc)7Twc/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 011060 B6;129S4-Nanogtm1Jae/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 013781 B6;FVB-Tg(Myh6/NFAT-luc)1Jmol/J 010545 C.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ 012943 C57BL/6-Tg(Ins2-luc/EGFP/TK)300Kauf/J 010548 D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ 008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J 009638 FVB-Tg(GFAP-luc,GAPDH-rluc)172.9Mes/J 010588 FVB-Tg(Myh6/NFAT-luc)1Jmol/J 018167 FVB-Tg(TERT-rluc)DJzhu/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 006206 FVB.129S6-Gt(ROSA)26Sortm2(HIF1A/luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 007800 FVB/N-Tg(Ins1-luc)VUPwrs/J 012370 FVB/NJ-Tg(Hspa1a-luc,-EGFP)2Chco/J 013116 NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J 010542 NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ 017596 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Smn1tm1Msd Tg(SMN2)89Ahmb Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J 017597 STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Smn1tm1Msd Tg(SMN2)89Ahmb Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J 017599 STOCK Tg(tetO-SMN2,-luc)#aAhmb/J 017600 STOCK Tg(tetO-SMN2,-luc)#bAhmb/JView Strains carrying other alleles of luc (33 strains)
View Research Applications
|Allele Name||transgene insertion 128, Joseph S Takahashi|
|Allele Type||Transgenic (Reporter)|
|Common Name(s)||Per1 Luc 128;|
|Mutation Made By||Dr. Joseph Takahashi, Univ Texas Southwestern Medical Ctr|
|Strain of Origin||CD-1|
|Site of Expression||Per1 promoter drives in vivo and in vitro expression of luciferase in a pattern recapitulating that of circadian rhythms in the suprachiasmatic nuclei.|
|Expressed Gene||luc, luciferase, firefly|
|Promoter||Per1, period circadian clock 1, mouse, laboratory|
|Molecular Note||6.75 kb of the mouse Per1 promoter (including exon 1, intron 1 and the untranslated portion of exon 2 up to the initiaton ATG) drives expression of luciferase. [MGI Ref ID J:73700]|
|Gene Symbol and Name||Tg(Per1-luc)128Jt, transgene insertion 128, Joseph S Takahashi|
Animal Health ReportsRoom Number FGB29
Breeding & Husbandry When maintained as a live colony, hemizygotes may be bred with wildtype mice. Mating System Noncarrier x Hemizygote (Female x Male) 22-SEP-11 Hemizygote x Noncarrier (Female x Male) 22-SEP-11 Diet Information LabDiet® 5K52/5K67
This strain is currently Under Development for Cryo.
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
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