Strain Name:

B6.129-Csnk1etm1Asil/J

Stock Number:

016183

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Availability:

Cryopreserved - Ready for recovery

These targeted mice carry the tau mutation in floxed exon 4 of the mouse Csnk1e (casein kinase 1, epsilon; also called Ck1 epsilon) gene. Homozygotes show a shortened circadian period of behavior.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. Joseph S. Takahashi,   Univ Texas Southwestern Medical Ctr

Description
The tau mutation of Syrian hamsters was the first mammalian circadian mutation discovered. These targeted mice carry the tau mutation in exon 4 of the mouse Csnk1e (casein kinase 1, epsilon; also called Ck1 epsilon) gene. Homozygotes show a shortened circadian period of behavior (in vivo) and suprachiasmatic nucleus firing rates (in vitro) due to accelerating Per1 (period homolog 1 (Drosophila))-dependent molecular feedback loops. LoxP sites located on either side of the mutant exon 4 enable tissue-specific cre excision of the catalytic domain to knock out expression. Homozygotes are viable and fertile with no overt deleterious phenotype.

Development
A targeting vector was used to introduce the tau mutation (nucleotide C12555T; amino acid C178T) to exon 4, a loxP site to intron 3, and a NeoTK (neomycin/thymidine kinase) selection cassette flanked by loxP sites to intron 4. R1 embyronic stem (ES) cells derived from (129X1/SvJ x 129S1/Sv)F1- Kitl+ mice were used to create mutant animals. The floxed selection cassette was deleted from homologously recombined ES cell clones by transient transfection with a cre-expressing plasmid, leaving exon 4 flanked by loxP sites. The resulting mice were backcrossed to C57BL/6J for more than 8 generations by the donating laboratory.

Related Strains

Strains carrying other alleles of Csnk1e
016184   B6.129(Cg)-Csnk1etm1.1Asil/J
010924   B6.129S4-Csnk1etm1.1Drw/J
010489   B6.129S4-Csnk1etm1Drw/J
View Strains carrying other alleles of Csnk1e     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Csnk1etm1Asil/Csnk1e+

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • behavior/neurological phenotype
  • shortened circadian period
    • significant shortening of circadian period of behavior in vivo and suprachiasmatic nucleus firing rates in vitro in a gene dose-dependent manner   (MGI Ref ID J:135186)
    • heterozygous mice have 1.8 hr shorter circadian period than wild-type   (MGI Ref ID J:135186)
    • mutant mice are viable and fertile with no overt deleterious phenotype   (MGI Ref ID J:135186)

Csnk1etm1Asil/Csnk1etm1Asil

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • behavior/neurological phenotype
  • shortened circadian period
    • significant shortening of circadian period of behavior in vivo and suprachiasmatic nucleus firing rates in vitro in a gene dose-dependent manner   (MGI Ref ID J:135186)
    • homozygous mice have 3.6 hr shorter circadian period than wild-type   (MGI Ref ID J:135186)
    • mutant mice are viable and fertile with no overt deleterious phenotype   (MGI Ref ID J:135186)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Circadian Rhythms
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Csnk1etm1Asil
Allele Name targeted mutation 1, Andrew S I Loudon
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) CK1epsilontau;
Mutation Made ByDr. Andrew Loudon,   University of Manchester
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Csnk1e, casein kinase 1, epsilon
Chromosome 15
Gene Common Name(s) AI426939; AI551861; AW457082; CK1epsilon; CKI epsilon; CKIepsilon; HCKIE; KC1epsilon; expressed sequence AI426939; expressed sequence AI551861; expressed sequence AW457082; tau;
Molecular Note A floxed neo cassette was inserted downstream of a modified exon 4 containing a C to T transition at base pair 12555 (C12555T) and an additional loxP site was inserted upstream of the modified exon 4. The neo cassette was removed by germ line, cre-mediated recombination that left the modified exon 4 floxed. The transition mimics a gain of function mutation identified in hamsters as having a destabilizing effect on Per1. [MGI Ref ID J:135186]

Genotyping

Genotyping Information

Genotyping Protocols

** Csnk1e*C178T, Pyrosequencing
Csnk1etm1Asil, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Meng QJ; Logunova L; Maywood ES; Gallego M; Lebiecki J; Brown TM; Sladek M; Semikhodskii AS; Glossop NR; Piggins HD; Chesham JE; Bechtold DA; Yoo SH; Takahashi JS; Virshup DM; Boot-Handford RP; Hastings MH; Loudon AS. 2008. Setting clock speed in mammals: the CK1 epsilon tau mutation in mice accelerates circadian pacemakers by selectively destabilizing PERIOD proteins. Neuron 58(1):78-88. [PubMed: 18400165]  [MGI Ref ID J:135186]

Additional References

Csnk1etm1Asil related

Maywood ES; Chesham JE; Meng QJ; Nolan PM; Loudon AS; Hastings MH. 2011. Tuning the Period of the Mammalian Circadian Clock: Additive and Independent Effects of CK1{varepsilon}Tau and Fbxl3Afh Mutations on Mouse Circadian Behavior and Molecular Pacemaking. J Neurosci 31(4):1539-44. [PubMed: 21273438]  [MGI Ref ID J:168543]

Maywood ES; Chesham JE; O'Brien JA; Hastings MH. 2011. A diversity of paracrine signals sustains molecular circadian cycling in suprachiasmatic nucleus circuits. Proc Natl Acad Sci U S A 108(34):14306-11. [PubMed: 21788520]  [MGI Ref ID J:175620]

Meng QJ; Maywood ES; Bechtold DA; Lu WQ; Li J; Gibbs JE; Dupre SM; Chesham JE; Rajamohan F; Knafels J; Sneed B; Zawadzke LE; Ohren JF; Walton KM; Wager TT; Hastings MH; Loudon AS. 2010. Entrainment of disrupted circadian behavior through inhibition of casein kinase 1 (CK1) enzymes. Proc Natl Acad Sci U S A 107(34):15240-5. [PubMed: 20696890]  [MGI Ref ID J:163807]

Mohawk JA; Baer ML; Menaker M. 2009. The methamphetamine-sensitive circadian oscillator does not employ canonical clock genes. Proc Natl Acad Sci U S A 106(9):3519-24. [PubMed: 19204282]  [MGI Ref ID J:146448]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes or homozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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