|Trpc3 (transient receptor potential cation channel, subfamily C, member 3) knockout mice may be useful in studies of mGluR-dependent synaptic transmission in cerebellar Purkinje cells and motor coordination.|
Type Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
In Trpc3 (transient receptor potential cation channel, subfamily C, member 3) deficient mice, both slow synaptic potentials and metabotropic glutamate receptor (mGluR)-mediated inward currents are completely absent. Synaptically mediated Ca2+ release signals from intracellular stores are unchanged. Homozygous knockout mice are viable, fertile and appear normal with regard to their general appearance, but show an impaired walking behavior. This strain may be useful in studies of mGluR-dependent synaptic transmission in cerebellar Purkinje cells and motor coordination.
A targeting vector was used to insert a loxP site in intron 6 and a loxP-flanked PGK-Neomycin cassette in intron 7. The mutation was created in 129S/SvEv-derived embryonic stem (ES) cells. Resultant mice were crossed with Meox2-cre mice (see Stock No. 003755) to excise exon 7 and the floxed neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.Spinocerebellar Ataxia 41; SCA41 (TRPC3)
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Trpc3tm1.1Akon/Trpc3tm1.1Akoninvolves: 129S/SvEv * 129S4/SvJaeSor
- behavior/neurological phenotype
- abnormal gait
- hind feet track outside of the path defined by the body diameter (MGI Ref ID J:139695)
- impaired limb coordination
- reduced ability to walk on an irregularly spaced ladder or on a thin elevated beam (MGI Ref ID J:139695)
- nervous system phenotype
- *normal* nervous system phenotype
Trpc3tm1.1Akon/Trpc3tm1.1Akoninvolves: 129S/SvEvView Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Ataxia (Movement) Defects
glutamate receptor: metabotropic
|Allele Name||targeted mutation 1.1, Arthur Konnerth|
|Allele Type||Targeted (Null/Knockout)|
|Mutation Made By||Dr. Arthur Konnerth, Technical University Munich|
|Strain of Origin||129S/SvEv|
|Gene Symbol and Name||Trpc3, transient receptor potential cation channel, subfamily C, member 3|
|Gene Common Name(s)||Mwk; SCA41; TRP3; Trcp3; TrpC3c; Trrp3; moonwalker; transient receptor protein 3;|
|Molecular Note||A loxP site was introduced into intron 6 and a floxed PGK-neo cassette was inserted into intron 7. The selection cassette was removed from properly targeted ES cells leaving exon 7 floxed. Mice with the floxed exon 7 were crossed to cre expressing miceto create the knockout allele. [MGI Ref ID J:139695]|
Feng S; Li H; Tai Y; Huang J; Su Y; Abramowitz J; Zhu MX; Birnbaumer L; Wang Y. 2013. Canonical transient receptor potential 3 channels regulate mitochondrial calcium uptake. Proc Natl Acad Sci U S A 110(27):11011-6. [PubMed: 23776229] [MGI Ref ID J:198713]
Gugger OS; Hartmann J; Birnbaumer L; Kapfhammer JP. 2012. P/Q-type and T-type calcium channels, but not type 3 transient receptor potential cation channels, are involved in inhibition of dendritic growth after chronic metabotropic glutamate receptor type 1 and protein kinase C activation in cerebellar Purkinje cells. Eur J Neurosci 35(1):20-33. [PubMed: 22188405] [MGI Ref ID J:184156]
Hartmann J; Dragicevic E; Adelsberger H; Henning HA; Sumser M; Abramowitz J; Blum R; Dietrich A; Freichel M; Flockerzi V; Birnbaumer L; Konnerth A. 2008. TRPC3 channels are required for synaptic transmission and motor coordination. Neuron 59(3):392-8. [PubMed: 18701065] [MGI Ref ID J:139695]
Klaiber M; Dankworth B; Kruse M; Hartmann M; Nikolaev VO; Yang RB; Volker K; Gassner B; Oberwinkler H; Feil R; Freichel M; Groschner K; Skryabin BV; Frantz S; Birnbaumer L; Pongs O; Kuhn M. 2011. A cardiac pathway of cyclic GMP-independent signaling of guanylyl cyclase A, the receptor for atrial natriuretic peptide. Proc Natl Acad Sci U S A 108(45):18500-5. [PubMed: 22027011] [MGI Ref ID J:180246]
Lee KP; Choi S; Hong JH; Ahuja M; Graham S; Ma R; So I; Shin DM; Muallem S; Yuan JP. 2014. Molecular determinants mediating gating of Transient Receptor Potential Canonical (TRPC) channels by stromal interaction molecule 1 (STIM1). J Biol Chem 289(10):6372-82. [PubMed: 24464579] [MGI Ref ID J:210682]
Lutas A; Birnbaumer L; Yellen G. 2014. Metabolism regulates the spontaneous firing of substantia nigra pars reticulata neurons via KATP and nonselective cation channels. J Neurosci 34(49):16336-47. [PubMed: 25471572] [MGI Ref ID J:218743]
Perez-Leighton CE; Schmidt TM; Abramowitz J; Birnbaumer L; Kofuji P. 2011. Intrinsic phototransduction persists in melanopsin-expressing ganglion cells lacking diacylglycerol-sensitive TRPC subunits. Eur J Neurosci 33(5):856-67. [PubMed: 21261756] [MGI Ref ID J:176849]
Phan PA; Tadros SF; Kim Y; Birnbaumer L; Housley GD. 2010. Developmental regulation of TRPC3 ion channel expression in the mouse cochlea. Histochem Cell Biol 133(4):437-48. [PubMed: 20229053] [MGI Ref ID J:161700]
Sekerkova G; Kim JA; Nigro MJ; Becker EB; Hartmann J; Birnbaumer L; Mugnaini E; Martina M. 2013. Early onset of ataxia in moonwalker mice is accompanied by complete ablation of type II unipolar brush cells and Purkinje cell dysfunction. J Neurosci 33(50):19689-94. [PubMed: 24336732] [MGI Ref ID J:205491]
Seo K; Rainer PP; Shalkey Hahn V; Lee DI; Jo SH; Andersen A; Liu T; Xu X; Willette RN; Lepore JJ; Marino JP Jr; Birnbaumer L; Schnackenberg CG; Kass DA. 2014. Combined TRPC3 and TRPC6 blockade by selective small-molecule or genetic deletion inhibits pathological cardiac hypertrophy. Proc Natl Acad Sci U S A 111(4):1551-6. [PubMed: 24453217] [MGI Ref ID J:206567]
Tano JY; Lee RH; Vazquez G. 2012. Involvement of calmodulin and calmodulin kinase II in tumor necrosis factor alpha-induced survival of bone marrow derived macrophages. Biochem Biophys Res Commun 427(1):178-84. [PubMed: 22989752] [MGI Ref ID J:190105]
Tano JY; Smedlund K; Lee R; Abramowitz J; Birnbaumer L; Vazquez G. 2011. Impairment of survival signaling and efferocytosis in TRPC3-deficient macrophages. Biochem Biophys Res Commun 410(3):643-7. [PubMed: 21684255] [MGI Ref ID J:174948]
Tano JY; Solanki S; Lee RH; Smedlund K; Birnbaumer L; Vazquez G. 2014. Bone marrow deficiency of TRPC3 channel reduces early lesion burden and necrotic core of advanced plaques in a mouse model of atherosclerosis. Cardiovasc Res 101(1):138-44. [PubMed: 24101197] [MGI Ref ID J:219544]
Wong AC; Birnbaumer L; Housley GD. 2013. Canonical transient receptor potential channel subtype 3-mediated hair cell Ca(2+) entry regulates sound transduction and auditory neurotransmission. Eur J Neurosci 37(9):1478-86. [PubMed: 23442051] [MGI Ref ID J:214902]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2225.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2892.50
Cryorecovery - Standard.
Progeny testing is not required.
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.