|Trpc3 (transient receptor potential cation channel, subfamily C, member 3) knockout mice may be useful in studies of mGluR-dependent synaptic transmission in cerebellar Purkinje cells and motor coordination.|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
In Trpc3 (transient receptor potential cation channel, subfamily C, member 3) deficient mice, both slow synaptic potentials and metabotropic glutamate receptor (mGluR)-mediated inward currents are completely absent. Synaptically mediated Ca2+ release signals from intracellular stores are unchanged. Homozygous knockout mice are viable, fertile and appear normal with regard to their general appearance, but show an impaired walking behavior. This strain may be useful in studies of mGluR-dependent synaptic transmission in cerebellar Purkinje cells and motor coordination.
A targeting vector was used to insert a loxP site in intron 6 and a loxP-flanked PGK-Neomycin cassette in intron 7. The mutation was created in 129S/SvEv-derived embryonic stem (ES) cells. Resultant mice were crossed with Meox2-cre mice (see Stock No. 003755) to excise exon 7 and the floxed neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Trpc3tm1.1Akon/Trpc3tm1.1Akoninvolves: 129S/SvEv * 129S4/SvJaeSor
- behavior/neurological phenotype
- abnormal gait
- hind feet track outside of the path defined by the body diameter (MGI Ref ID J:139695)
- impaired limb coordination
- reduced ability to walk on an irregularly spaced ladder or on a thin elevated beam (MGI Ref ID J:139695)
- nervous system phenotype
- *normal* nervous system phenotype
Trpc3tm1.1Akon/Trpc3tm1.1Akoninvolves: 129S/SvEvView Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Ataxia (Movement) Defects
glutamate receptor: metabotropic
|Allele Name||targeted mutation 1.1, Arthur Konnerth|
|Allele Type||Targeted (Null/Knockout)|
|Mutation Made By||Dr. Arthur Konnerth, Technical University Munich|
|Strain of Origin||129S/SvEv|
|Gene Symbol and Name||Trpc3, transient receptor potential cation channel, subfamily C, member 3|
|Gene Common Name(s)||Mwk; TRP3; Trcp3; TrpC3c; Trrp3; moonwalker; transient receptor protein 3;|
|Molecular Note||A loxP site was introduced into intron 6 and a floxed PGK-neo cassette was inserted into intron 7. The selection cassette was removed from properly targeted ES cells leaving exon 7 floxed. Mice with the floxed exon 7 were crossed to cre expressing miceto create the knockout allele. [MGI Ref ID J:139695]|
Feng S; Li H; Tai Y; Huang J; Su Y; Abramowitz J; Zhu MX; Birnbaumer L; Wang Y. 2013. Canonical transient receptor potential 3 channels regulate mitochondrial calcium uptake. Proc Natl Acad Sci U S A 110(27):11011-6. [PubMed: 23776229] [MGI Ref ID J:198713]
Gugger OS; Hartmann J; Birnbaumer L; Kapfhammer JP. 2012. P/Q-type and T-type calcium channels, but not type 3 transient receptor potential cation channels, are involved in inhibition of dendritic growth after chronic metabotropic glutamate receptor type 1 and protein kinase C activation in cerebellar Purkinje cells. Eur J Neurosci 35(1):20-33. [PubMed: 22188405] [MGI Ref ID J:184156]
Hartmann J; Dragicevic E; Adelsberger H; Henning HA; Sumser M; Abramowitz J; Blum R; Dietrich A; Freichel M; Flockerzi V; Birnbaumer L; Konnerth A. 2008. TRPC3 channels are required for synaptic transmission and motor coordination. Neuron 59(3):392-8. [PubMed: 18701065] [MGI Ref ID J:139695]
Klaiber M; Dankworth B; Kruse M; Hartmann M; Nikolaev VO; Yang RB; Volker K; Gassner B; Oberwinkler H; Feil R; Freichel M; Groschner K; Skryabin BV; Frantz S; Birnbaumer L; Pongs O; Kuhn M. 2011. A cardiac pathway of cyclic GMP-independent signaling of guanylyl cyclase A, the receptor for atrial natriuretic peptide. Proc Natl Acad Sci U S A 108(45):18500-5. [PubMed: 22027011] [MGI Ref ID J:180246]
Lee KP; Choi S; Hong JH; Ahuja M; Graham S; Ma R; So I; Shin DM; Muallem S; Yuan JP. 2014. Molecular determinants mediating gating of Transient Receptor Potential Canonical (TRPC) channels by stromal interaction molecule 1 (STIM1). J Biol Chem 289(10):6372-82. [PubMed: 24464579] [MGI Ref ID J:210682]
Perez-Leighton CE; Schmidt TM; Abramowitz J; Birnbaumer L; Kofuji P. 2011. Intrinsic phototransduction persists in melanopsin-expressing ganglion cells lacking diacylglycerol-sensitive TRPC subunits. Eur J Neurosci 33(5):856-67. [PubMed: 21261756] [MGI Ref ID J:176849]
Phan PA; Tadros SF; Kim Y; Birnbaumer L; Housley GD. 2010. Developmental regulation of TRPC3 ion channel expression in the mouse cochlea. Histochem Cell Biol 133(4):437-48. [PubMed: 20229053] [MGI Ref ID J:161700]
Sekerkova G; Kim JA; Nigro MJ; Becker EB; Hartmann J; Birnbaumer L; Mugnaini E; Martina M. 2013. Early onset of ataxia in moonwalker mice is accompanied by complete ablation of type II unipolar brush cells and Purkinje cell dysfunction. J Neurosci 33(50):19689-94. [PubMed: 24336732] [MGI Ref ID J:205491]
Seo K; Rainer PP; Shalkey Hahn V; Lee DI; Jo SH; Andersen A; Liu T; Xu X; Willette RN; Lepore JJ; Marino JP Jr; Birnbaumer L; Schnackenberg CG; Kass DA. 2014. Combined TRPC3 and TRPC6 blockade by selective small-molecule or genetic deletion inhibits pathological cardiac hypertrophy. Proc Natl Acad Sci U S A 111(4):1551-6. [PubMed: 24453217] [MGI Ref ID J:206567]
Tano JY; Lee RH; Vazquez G. 2012. Involvement of calmodulin and calmodulin kinase II in tumor necrosis factor alpha-induced survival of bone marrow derived macrophages. Biochem Biophys Res Commun 427(1):178-84. [PubMed: 22989752] [MGI Ref ID J:190105]
Tano JY; Smedlund K; Lee R; Abramowitz J; Birnbaumer L; Vazquez G. 2011. Impairment of survival signaling and efferocytosis in TRPC3-deficient macrophages. Biochem Biophys Res Commun 410(3):643-7. [PubMed: 21684255] [MGI Ref ID J:174948]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintained as a live colony, heterozygotes or homozygotes may be bred.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2140.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2782.00
Cryorecovery - Standard.
Progeny testing is not required.
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