Strain Name:

B6;129S-Trpc3tm1.1Akon/J

Stock Number:

016188

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Availability:

Cryopreserved - Ready for recovery

Trpc3 (transient receptor potential cation channel, subfamily C, member 3) knockout mice may be useful in studies of mGluR-dependent synaptic transmission in cerebellar Purkinje cells and motor coordination.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Joseph S. Takahashi,   Univ Texas Southwestern Medical Ctr

Description
In Trpc3 (transient receptor potential cation channel, subfamily C, member 3) deficient mice, both slow synaptic potentials and metabotropic glutamate receptor (mGluR)-mediated inward currents are completely absent. Synaptically mediated Ca2+ release signals from intracellular stores are unchanged. Homozygous knockout mice are viable, fertile and appear normal with regard to their general appearance, but show an impaired walking behavior. This strain may be useful in studies of mGluR-dependent synaptic transmission in cerebellar Purkinje cells and motor coordination.

Development
A targeting vector was used to insert a loxP site in intron 6 and a loxP-flanked PGK-Neomycin cassette in intron 7. The mutation was created in 129S/SvEv-derived embryonic stem (ES) cells. Resultant mice were crossed with Meox2-cre mice (see Stock No. 003755) to excise exon 7 and the floxed neomycin cassette. This strain was maintained on a mixed C57BL/6 and 129 genetic background by the donating laboratory.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Trpc3tm1.1Akon/Trpc3tm1.1Akon

        involves: 129S/SvEv * 129S4/SvJaeSor
  • behavior/neurological phenotype
  • abnormal gait
    • hind feet track outside of the path defined by the body diameter   (MGI Ref ID J:139695)
  • impaired limb coordination
    • reduced ability to walk on an irregularly spaced ladder or on a thin elevated beam   (MGI Ref ID J:139695)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • normal size and layered structure of cerebellum   (MGI Ref ID J:139695)
    • normal Purkinje cell morphology   (MGI Ref ID J:139695)
    • abnormal excitatory postsynaptic currents
      • the mGluR1 (Grm1) mediated slow EPSC is completely abolished   (MGI Ref ID J:139695)
      • synaptically evoked dendritic calcium ion signal is unaffected   (MGI Ref ID J:139695)

Trpc3tm1.1Akon/Trpc3tm1.1Akon

        involves: 129S/SvEv
  • vision/eye phenotype
  • abnormal eye physiology
    • intrinsically photosensitive retinal ganglion cells exhibit decreased firing frequency at most light intensities compared with wild-type cells   (MGI Ref ID J:176849)
    • however, sensitivity is normal   (MGI Ref ID J:176849)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Ataxia (Movement) Defects
Receptor Defects
      glutamate receptor: metabotropic

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Trpc3tm1.1Akon
Allele Name targeted mutation 1.1, Arthur Konnerth
Allele Type Targeted (Null/Knockout)
Common Name(s) Trpc3-;
Mutation Made ByDr. Arthur Konnerth,   Technical University Munich
Strain of Origin129S/SvEv
Gene Symbol and Name Trpc3, transient receptor potential cation channel, subfamily C, member 3
Chromosome 3
Gene Common Name(s) Mwk; TRP3; Trcp3; TrpC3c; Trrp3; moonwalker; transient receptor protein 3;
Molecular Note A loxP site was introduced into intron 6 and a floxed PGK-neo cassette was inserted into intron 7. The selection cassette was removed from properly targeted ES cells leaving exon 7 floxed. Mice with the floxed exon 7 were crossed to cre expressing miceto create the knockout allele. [MGI Ref ID J:139695]

Genotyping

Genotyping Information

Genotyping Protocols

Trpc3tm1.1Akonalternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Trpc3tm1.1Akon related

Feng S; Li H; Tai Y; Huang J; Su Y; Abramowitz J; Zhu MX; Birnbaumer L; Wang Y. 2013. Canonical transient receptor potential 3 channels regulate mitochondrial calcium uptake. Proc Natl Acad Sci U S A 110(27):11011-6. [PubMed: 23776229]  [MGI Ref ID J:198713]

Gugger OS; Hartmann J; Birnbaumer L; Kapfhammer JP. 2012. P/Q-type and T-type calcium channels, but not type 3 transient receptor potential cation channels, are involved in inhibition of dendritic growth after chronic metabotropic glutamate receptor type 1 and protein kinase C activation in cerebellar Purkinje cells. Eur J Neurosci 35(1):20-33. [PubMed: 22188405]  [MGI Ref ID J:184156]

Hartmann J; Dragicevic E; Adelsberger H; Henning HA; Sumser M; Abramowitz J; Blum R; Dietrich A; Freichel M; Flockerzi V; Birnbaumer L; Konnerth A. 2008. TRPC3 channels are required for synaptic transmission and motor coordination. Neuron 59(3):392-8. [PubMed: 18701065]  [MGI Ref ID J:139695]

Klaiber M; Dankworth B; Kruse M; Hartmann M; Nikolaev VO; Yang RB; Volker K; Gassner B; Oberwinkler H; Feil R; Freichel M; Groschner K; Skryabin BV; Frantz S; Birnbaumer L; Pongs O; Kuhn M. 2011. A cardiac pathway of cyclic GMP-independent signaling of guanylyl cyclase A, the receptor for atrial natriuretic peptide. Proc Natl Acad Sci U S A 108(45):18500-5. [PubMed: 22027011]  [MGI Ref ID J:180246]

Lee KP; Choi S; Hong JH; Ahuja M; Graham S; Ma R; So I; Shin DM; Muallem S; Yuan JP. 2014. Molecular determinants mediating gating of Transient Receptor Potential Canonical (TRPC) channels by stromal interaction molecule 1 (STIM1). J Biol Chem 289(10):6372-82. [PubMed: 24464579]  [MGI Ref ID J:210682]

Perez-Leighton CE; Schmidt TM; Abramowitz J; Birnbaumer L; Kofuji P. 2011. Intrinsic phototransduction persists in melanopsin-expressing ganglion cells lacking diacylglycerol-sensitive TRPC subunits. Eur J Neurosci 33(5):856-67. [PubMed: 21261756]  [MGI Ref ID J:176849]

Phan PA; Tadros SF; Kim Y; Birnbaumer L; Housley GD. 2010. Developmental regulation of TRPC3 ion channel expression in the mouse cochlea. Histochem Cell Biol 133(4):437-48. [PubMed: 20229053]  [MGI Ref ID J:161700]

Sekerkova G; Kim JA; Nigro MJ; Becker EB; Hartmann J; Birnbaumer L; Mugnaini E; Martina M. 2013. Early onset of ataxia in moonwalker mice is accompanied by complete ablation of type II unipolar brush cells and Purkinje cell dysfunction. J Neurosci 33(50):19689-94. [PubMed: 24336732]  [MGI Ref ID J:205491]

Seo K; Rainer PP; Shalkey Hahn V; Lee DI; Jo SH; Andersen A; Liu T; Xu X; Willette RN; Lepore JJ; Marino JP Jr; Birnbaumer L; Schnackenberg CG; Kass DA. 2014. Combined TRPC3 and TRPC6 blockade by selective small-molecule or genetic deletion inhibits pathological cardiac hypertrophy. Proc Natl Acad Sci U S A 111(4):1551-6. [PubMed: 24453217]  [MGI Ref ID J:206567]

Tano JY; Lee RH; Vazquez G. 2012. Involvement of calmodulin and calmodulin kinase II in tumor necrosis factor alpha-induced survival of bone marrow derived macrophages. Biochem Biophys Res Commun 427(1):178-84. [PubMed: 22989752]  [MGI Ref ID J:190105]

Tano JY; Smedlund K; Lee R; Abramowitz J; Birnbaumer L; Vazquez G. 2011. Impairment of survival signaling and efferocytosis in TRPC3-deficient macrophages. Biochem Biophys Res Commun 410(3):643-7. [PubMed: 21684255]  [MGI Ref ID J:174948]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintained as a live colony, heterozygotes or homozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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