Description

Strain Information

Former Names B6.129S-Dnmt3ltm2Bes/J    (Changed: 05-MAY-11 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Wild-type x Heterozygote         (Female x Male)   20-OCT-11 Mating System Heterozygote x Wild-type         (Female x Male)   20-OCT-11 Species laboratory mouse Generation F?+ (09-MAY-11) Generation Definitions   Donating Investigator Timothy H. Bestor,   Columbia University

Description
Dnmt3l (DNA (cytosine-5-)-methyltransferase 3-like) is an enzymatically inactive regulatory factor that establishes patterns of DNA methylation inactivation during embryogenesis. A cysteine-rich domain of this protein interacts with the extreme amino terminal of histone H3. Data indicate that DNMT3L recognizes histone H3 tails that are unmethylated at lysine 4 and induces de novo DNA methylation by recruitment or activation of DNMT3A (DNA methyltransferase 3A). In this mutant, a tandem His₆-Flag tag was introduced to the N terminus of the endogenous Dnmt3l mouse gene. Anti-flag immunoprecipitation can be used to isolate and analyse DNMT3L complexes (e.g. DNMT3A, DNMT3B, core histones). Unlike Dnmt3l knockout mice, both males and females carrying this knock-in allele have no overt phenotype and are viable and fertile in the homozygous state, suggesting normal functionality of the targeted gene.

Development
A targeting vector was designed to introduce a tandem His₆-Flag tag immediately after the first four amino acids (encoded by exon 2) of the mouse Dnmt3l gene. A loxP-flanked PGK-neomycin cassette was placed in intron 4. The vector was introduced to 129/SvEv-derived embryonic stem cells and transient exposure to Cre recombinase excised the neomycin cassette. Resultant mice were backcrossed once to C57BL/6 to establish the colony. Mutant mice were bred together for several generations prior to sending agouti mice to The Jackson Laboratory Repository.

Control Information

	Control
	Wild-type from the colony
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Dnmt3l

004267

B6.129S6-Dnmt3ltm1Bes/J

View Strains carrying other alleles of Dnmt3l     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Dnmt3l^tm2Bes/Dnmt3l^tm2Bes

        involves: 129/Sv

• normal phenotype
• no abnormal phenotype detected
  • mice are normal in phenotype and both sexes are fertile   (MGI Ref ID J:123743)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Dnmt3ltm2Bes
Allele Name		targeted mutation 2, Timothy H Bestor
Allele Type		Targeted (Reporter)
Common Name(s)		Dnmt3lTag;
Mutation Made By		Timothy Bestor,   Columbia University
Strain of Origin		129/Sv
Gene Symbol and Name		Dnmt3l, DNA (cytosine-5-)-methyltransferase 3-like
Chromosome		10
Gene Common Name(s)		D6Ertd14e; DNA segment, Chr 6, ERATO Doi 14, expressed; ecat7;
Molecular Note		A targeting vector was used to insert an N-terminal His6-FLAG epitope tag after the first four amino acids of the locus. A floxed pgk-neo included in the vector was subsequently removed via transient cre expression. [MGI Ref ID J:123743]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ooi SK; Qiu C; Bernstein E; Li K; Jia D; Yang Z; Erdjument-Bromage H; Tempst P; Lin SP; Allis CD; Cheng X; Bestor TH. 2007. DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA. Nature 448(7154):714-7. [PubMed: 17687327]  [MGI Ref ID J:123743]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, male and female homozygotes or heterozygotes may be bred.
Mating System	Wild-type x Heterozygote         (Female x Male)   20-OCT-11
	Heterozygote x Wild-type         (Female x Male)   20-OCT-11

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Awaiting Transfer from the Donor.

View All Strains Awaiting Transfer from the Donor, In Progress and On Hold

Control Information

	Control
	Wild-type from the colony
	101043 B6129SF1/J	(approximate)
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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