Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Konrad Hochedlinger,   Massachusetts General Hospital / Harvard

Description
The coding region of the Nanog homeobox (Nanog) is replaced by a GFP-IRES-puro-cassette in these mice, abolishing gene expression. NANOG is normally expressed in embryonic stem cells (ESCs) and functions as a key factor in maintaining pluripotency. Heterozygous mutant mice are viable and fertile, while homozygotes are embryonic lethal. Mouse embryonic fibroblasts (MEFs) derived from these GFP reporter mice are useful for identifying induced pluripotent stem (iPS) cell formation.

Development
A targeting vector was designed to replace the Nanog homeobox (Nanog) coding region with an enhanced green fluorescent protein (GFP), an internal ribosome entry site (IRES), a puromycin (puro) resistance cassette and a polyadenylation signal. This construct was electroporated into (C57BL/6 x 129S4/SvJae)F1-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient (C57BL/6 x DBA)F2 blastocysts and chimeric males were bred with 129S4/SvJae females. These Nanog-GFP mice were intercrossed and maintained on a mixed background. Upon arrival at The Jackson Laboratory Repository, mice were bred with 129S1/SvImJ inbred mice (Stock No. 002448) for at least one generation.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Nanog

011060

B6;129S4-Nanogtm1Jae/J

View Strains carrying other alleles of Nanog     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Nanog^tm1Hoch/Nanog⁺

        involves: 129S4/SvJae * C57BL/6

• no phenotypic analysis
• *normal* no phenotypic analysis
  • mice were used to monitor the generation of iPS cells   (MGI Ref ID J:158493)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cell Biology Research
Transcriptional Regulation

Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects
      hematopoietic defects
Lymphoid Tissue Defects
      hematopoietic defects

Hematological Research
Hematopoietic Defects

Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects
      hematopoietic development

Research Tools
Fluorescent Proteins
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: transcriptional activation
      Tissue/Cell Markers
Hematological Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Nanogtm1Hoch
Allele Name		targeted mutation 1, Konrad Hochedlinger
Allele Type		Targeted (Reporter)
Common Name(s)		Nanog-GFP;
Mutation Made By		Konrad Hochedlinger,   Massachusetts General Hospital / Harvard
Strain of Origin		(C57BL/6 x 129S4/SvJae)F1
Site of Expression		GFP is expressed in embryonic fibroblasts (MEFs) derived from these mice.
Gene Symbol and Name		Nanog, Nanog homeobox
Chromosome		6
Gene Common Name(s)		2410002E02Rik; ENK; RIKEN cDNA 2410002E02 gene; ecat4;
Molecular Note		The coding region was replaced with an GFP-iresPuro cassette. [MGI Ref ID J:158493] [MGI Ref ID J:95814]

Genotyping

Genotyping Information

Genotyping Protocols

Nanog^tm1Hoch, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Maherali N; Sridharan R; Xie W; Utikal J; Eminli S; Arnold K; Stadtfeld M; Yachechko R; Tchieu J; Jaenisch R; Plath K; Hochedlinger K. 2007. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 1(1):55-70. [PubMed: 18371336]  [MGI Ref ID J:158493]

Additional References

Nanog^tm1Hoch related

Brambrink T; Foreman R; Welstead GG; Lengner CJ; Wernig M; Suh H; Jaenisch R. 2008. Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2(2):151-9. [PubMed: 18371436]  [MGI Ref ID J:149805]

Hatano SY; Tada M; Kimura H; Yamaguchi S; Kono T; Nakano T; Suemori H; Nakatsuji N; Tada T. 2005. Pluripotential competence of cells associated with Nanog activity. Mech Dev 122(1):67-79. [PubMed: 15582778]  [MGI Ref ID J:95814]

Markoulaki S; Hanna J; Beard C; Carey BW; Cheng AW; Lengner CJ; Dausman JA; Fu D; Gao Q; Wu S; Cassady JP; Jaenisch R. 2009. Transgenic mice with defined combinations of drug-inducible reprogramming factors. Nat Biotechnol 27(2):169-71. [PubMed: 19151700]  [MGI Ref ID J:158492]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, heterozygous mice may be bred with wildtype mice from the colony. The donating investigator reports that homozygous mice are embryonic lethal.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 12-AUG-13

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.