Strain Name:

B6.Cg-Tg(Col1a2-cre/ERT)7Cpd/J

Stock Number:

016237

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Common Names: Col1a2-CreER;    
This Col1a2 Cre-ER mutant mouse strain carries a transgene with tamoxifen inducible Cre recombinase and may be useful for generating fibroblast specific targeted mutants for studies of fibrotic diseases, wound healing and connective tissue physiology, as well as fibroblast lineage fate mapping.

Description

Strain Information

Type Congenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   18-OCT-11
Mating SystemHemizygote x Noncarrier         (Female x Male)   18-OCT-11
Specieslaboratory mouse
GenerationN11+N1F4 (13-DEC-13)
Generation Definitions
 
Donating Investigator Benoit de Crombrugghe,   UT MD Anderson Cancer Center

Description
These transgenic mice express a tamoxifen-inducible Cre recombinase driven by the mouse Col1a2, collagen, type I, alpha 2, promoter. The transgene insert contains a fusion product involving Cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/Esr1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. When crossed with a strain containing a loxP site flanked sequence, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions of the flanked sequence. Tamoxifen administration in double mutant mice, carrying this transgene and a beta-galactosidase reporter, induces Cre recombination in dermal and visceral fibroblasts. Transgenic embryos (aged 13.5 embryonic days) exhibit inducible Cre recombinase activity in connective tissue of the spleen, lung, skin, fascia, perimysial tissues, blood vessel walls, surrounding gut wall smooth muscle cells, and in the periosteum. Neonatal tamoxifen induction results in Cre recombinase activity in fibroblastic structures of the dermis, lung, pericardial connective tissue, blood vessel wall, splenic capsule, and glomerular mesangial cells. The activity pattern is similar to transgene expression patterns observed in mutant mice carrying transgenes with the same Col1a2 promoter. Transgenic mice also express the heat stable human placental isoform of alkaline phosphatase as a dicistronic transgene product, with strong expression detected in skin. The Donating Investigator reports that homozygous mice do not breed well. Hemizygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

Development
A transgenic construct containing containing sequence encoding cre/Esr1, IRES sequence and sequence encoding human placental alkaline phosphatase, under the control of the regulatory elements of the mouse Col1a2, collagen, type I, alpha 2, gene (6 kb of the upstream 5' flanking region upstream of the transcription start site and endogenous minimal promoter) was injected into fertilized B6D2F1/J mouse eggs. Founder line 7 was subsequently established. The donating investigator reported that these mice were then backcrossed to the C57BL/6 background for 10 generations (see SNP note below). During backcrossing, the Y chromosome may not have been fixed to the C57BL/6 genetic background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

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007248   B6.129(FVB)-Col1a2tm1.1Mcbr/J
001815   B6C3Fe a/a-Col1a2oim/J
View Strains carrying other alleles of Col1a2     (2 strains)

Strains carrying other alleles of cre/ERT2
017611   129-Mcm2tm1(cre/ERT2)Scpr/J
009575   B6(129S4)-Et(cre/ERT2)119Rdav/J
009580   B6(129S4)-Et(cre/ERT2)1382Rdav/J
012688   B6(129S4)-Et(cre/ERT2)13866Rdav/J
009581   B6(129S4)-Et(cre/ERT2)1642Rdav/J
009582   B6(129S4)-Et(cre/ERT2)1645Rdav/J
009583   B6(129S4)-Et(cre/ERT2)1957Rdav/J
009584   B6(129S4)-Et(cre/ERT2)2007Rdav/J
009585   B6(129S4)-Et(cre/ERT2)2047Rdav/J
009574   B6(129S4)-Et(cre/ERT2)21Rdav/J
009577   B6(129S4)-Et(cre/ERT2)296Rdav/J
009578   B6(129S4)-Et(cre/ERT2)398Rdav/J
009573   B6(129S4)-Et(cre/ERT2)4Rdav/J
010688   B6(129S4)-Et(cre/ERT2)6691Rdav/J
010689   B6(129S4)-Et(cre/ERT2)6959Rdav/J
010690   B6(129S4)-Et(cre/ERT2)7089Rdav/J
010691   B6(129S4)-Et(cre/ERT2)7149Rdav/J
010692   B6(129S4)-Et(cre/ERT2)7381Rdav/J
010693   B6(129S4)-Et(cre/ERT2)8120Rdav/J
010694   B6(129S4)-Et(cre/ERT2)8131Rdav/J
009579   B6(129S4)-Et(cre/ERT2)837Rdav/J
010695   B6(129S4)-Et(cre/ERT2)9699Rdav/J
010696   B6(129S4)-Et(icre/ERT2)10596Rdav/J
010697   B6(129S4)-Et(icre/ERT2)10727Rdav/J
012689   B6(129S4)-Et(icre/ERT2)14163Rdav/J
012690   B6(129S4)-Et(icre/ERT2)14208Rdav/J
012694   B6(129S4)-Et(icre/ERT2)14915Rdav/J
022356   B6(129X1)-Tg(Cd4-cre/ERT2)11Gnri/J
013730   B6(Cg)-Calb2tm2.1(cre/ERT2)Zjh/J
010705   B6(Cg)-Dlx5tm1(cre/ERT2)Zjh/J
013048   B6(Cg)-Etv1tm1.1(cre/ERT2)Zjh/J
010776   B6(Cg)-Lhx6tm1(cre/ERT2)Zjh/J
010777   B6(Cg)-Pvalbtm1(cre/ERT2)Zjh/J
010708   B6(Cg)-Ssttm1(cre/ERT2)Zjh/J
026512   B6(Cg)-Tg(TPO-cre/ERT2)1139Tyj/J
021881   B6.129(Cg)-Arctm1.1(cre/ERT2)Luo/J
018867   B6.129(Cg)-Axin2tm1(cre/ERT2)Rnu/J
021882   B6.129(Cg)-Fostm1.1(cre/ERT2)Luo/J
008463   B6.129-Gt(ROSA)26Sortm1(cre/ERT2)Tyj/J
020940   B6.129P2(C)-Cx3cr1tm2.1(cre/ERT2)Jung/J
021160   B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT)Litt/WganJ
027116   B6.129P2-Cd19tm1(cre/ERT2)Rsky/J
008875   B6.129P2-Lgr5tm1(cre/ERT2)Cle/J
016934   B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
016222   B6.129S(Cg)-Id2tm1.1(cre/ERT2)Blh/ZhuJ
005623   B6.129S6-Shhtm2(cre/ERT2)Cjt/J
019148   B6.Cg-Acantm1(cre/ERT2)Crm/J
012360   B6.Cg-Erbb4tm1.1(cre/ERT2)Aibs/J
023676   B6.Cg-Hprttm331(Ple275-icre/ERT2)Ems/Mmjax
023677   B6.Cg-Hprttm332(Ple272-icre/ERT2)Ems/Mmjax
023678   B6.Cg-Hprttm333(Ple281-icre/ERT2)Ems/Mmjax
023679   B6.Cg-Hprttm334(Ple279-icre/ERT2)Ems/Mmjax
023680   B6.Cg-Hprttm335(Ple277-icre/ERT2)Ems/Mmjax
023681   B6.Cg-Hprttm336(Ple134-icre/ERT2)Ems/Mmjax
023682   B6.Cg-Hprttm337(Ple285-icre/ERT2)Ems/Mmjax
023683   B6.Cg-Hprttm338(Ple284-icre/ERT2)Ems/Mmjax
023684   B6.Cg-Hprttm339(Ple280-icre/ERT2)Ems/Mmjax
023685   B6.Cg-Hprttm340(Ple252-icre/ERT2)Ems/Mmjax
023686   B6.Cg-Hprttm341(Ple273-icre/ERT2)Ems/Mmjax
023687   B6.Cg-Hprttm342(Ple282-icre/ERT2)Ems/Mmjax
023688   B6.Cg-Hprttm343(Ple270-icre/ERT2)Ems/Mmjax
023689   B6.Cg-Hprttm344(Ple5-icre/ERT2)Ems/Mmjax
023690   B6.Cg-Hprttm345(icre/ERT2)Ems/Mmjax
023691   B6.Cg-Hprttm346(Ple264-icre/ERT2)Ems/Mmjax
023692   B6.Cg-Hprttm347(Ple155-icre/ERT2)Ems/Mmjax
023693   B6.Cg-Hprttm348(Ple265-icre/ERT2)Ems/Mmjax
023694   B6.Cg-Hprttm349(Ple293-icre/ERT2)Ems/Mmjax
023695   B6.Cg-Hprttm350(Ple232-icre/ERT2)Ems/Mmjax
023696   B6.Cg-Hprttm351(Ple198-icre/ERT2)Ems/Mmjax
023697   B6.Cg-Hprttm352(Ple291-icre/ERT2)Ems/Mmjax
022861   B6.Cg-Nxph4tm1.1(cre/ERT2)Hze/J
017763   B6.Cg-Pax7tm1(cre/ERT2)Gaka/J
022862   B6.Cg-Penktm1.1(cre/ERT2)Hze/J
021189   B6.Cg-Pvalbtm1.1(cre/ERT2)Hze/J
022865   B6.Cg-Trib2tm1.1(cre/ERT2)Hze/J
022357   B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ
026516   B6.Cg-Tg(Avil-cre/ERT2)AJwo/J
022390   B6.Cg-Tg(CDX2-Cre/ERT2)752Erf/J
016241   B6.Cg-Tg(Col1a1-cre/ERT2)1Crm/J
024709   B6.Cg-Tg(Ins1-cre/ERT)1Lphi/J
007606   B6.Cg-Tg(Thy1-cre/ERT2,-EYFP)AGfng/J
021961   B6;129-Abcg2tm3.1(cre/ERT2)Bsor/J
010531   B6;129-Bmi1tm1(cre/ERT)Mrc/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/ERT)Nat/J
010557   B6;129-Gt(ROSA)26Sortm3(rtTA,tetO-cre/ERT)Nat/J
024475   B6;129-Myod1tm1.1(cre/ERT,TVA)Gcg/J
008363   B6;129-Nefltm1(cre/ERT)Nat/J
012476   B6;129-Pax7tm2.1(cre/ERT2)Fan/J
009600   B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J
008532   B6;129-Thtm1(cre/Esr1)Nat/J
008531   B6;129-Vamp2tm1(cre/ERT)Nat/J
010985   B6;129P-Klf3tm1(cre/ERT2)Pzg/J
015854   B6;129P2-Foxl2tm1(GFP/cre/ERT2)Pzg/J
014541   B6;129S-Nos1tm1.1(cre/ERT2)Zjh/J
010987   B6;129S-Sox18tm1(GFP/cre/ERT2)Pzg/J
017593   B6;129S-Sox2tm1(cre/ERT2)Hoch/J
014551   B6;129S4-Dlx1tm1(cre/ERT2)Zjh/J
012464   B6;129S4-Foxd1tm2(GFP/cre/ERT2)Amc/J
009576   B6;129S4-Et(cre/ERT2)278Rdav/J
024948   B6;129S6-Gdnftm1(cre/ERT2)Cos/J
012362   B6;129S6-Tg(Camk2a-cre/ERT2)1Aibs/J
017495   B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J
014638   B6;129X1-Cldn6tm1(cre/ERT2)Dam/J
025809   B6;CBA-Tg(Fgfr3-icre/ERT2)4-2Wdr/J
025808   B6;CBA-Tg(Nkx2-1-cre/ERT2)1Kess/J
024768   B6;CBA-Tg(Upk2-icre/ERT2)1Ccc/J
007610   B6;SJL-Tg(Thy1-cre/ERT2,-EYFP)VGfng/J
016225   B6N.129S6(Cg)-Scgb1a1tm1(cre/ERT)Blh/J
010550   B6N.FVB-Tg(Penk-glc-2-cre/ERT2)2And/J
017743   B6N;129S-Prom1tm1(cre/ERT2)Gilb/J
025124   C57BL/6-Tg(Adipoq-cre/ERT2)1Soff/J
025867   C57BL/6J-Tg(Neurod1-cre/ERT2)M1Fcal/J
006774   FVB-Tg(Col2a1-cre/ERT)KA3Smac/J
024760   FVB/N-Tg(Ddx4-cre/ERT2)1Dcas/J
012882   STOCK Ascl1tm1.1(Cre/ERT2)Jejo/J
012710   STOCK Ccktm2.1(cre/ERT2)Zjh/J
007924   STOCK En2tm4(cre/ERT2)Alj/J
016961   STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J
010702   STOCK Gad2tm1(cre/ERT2)Zjh/J
022135   STOCK Gbx2tm1.1(cre/ERT2)Jyhl/J
007913   STOCK Gli1tm3(cre/ERT2)Alj/J
024283   STOCK Hcn4tm2.1(cre/ERT2)Sev/J
017606   STOCK Hopxtm2.1(cre/ERT2)Joe/J
018418   STOCK Lrig1tm1.1(cre/ERT2)Rjc/J
014552   STOCK Nkx2-1tm1.1(cre/ERT2)Zjh/J
017536   STOCK Nkx6-2tm1(cre/ERT2)Fsh/J
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
017569   STOCK Polr2atm1(cre/ERT2)Bbd E4f1tm1.1Llca/J
017585   STOCK Polr2atm1(cre/ERT2)Bbd/J
022757   STOCK Prg4tm1(GFP/cre/ERT2)Abl/J
010912   STOCK Wt1tm2(cre/ERT2)Wtp/J
012691   STOCK Et(icre/ERT2)14374Rdav/J
012692   STOCK Et(icre/ERT2)14602Rdav/J
012693   STOCK Et(icre/ERT2)14624Rdav/J
007684   STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J
025736   STOCK Tg(Cela1-cre/ERT)1Lgdn/J
027681   STOCK Tg(Hnf1b-cre/ERT2)1Jfer/J
025520   STOCK Tg(T-cre/ERT2)1Lwd/J
View Strains carrying other alleles of cre/ERT2     (138 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      Cre Recombinase Expression: Inducible

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Col1a2-cre/ERT)7Cpd
Allele Name transgene insertion 7, Christopher P Denton
Allele Type Transgenic (Inducible, cre- or Flp-expressing)
Common Name(s) Col1a2 Cre-ER; Col1a2-Cre-ER(T); Col1a2CreERT; Tg(Col1a2-cre/ESR1)71Pcn; Tg(Col1a2-cre/ESR1)7Cpd;
Mutation Made By Zhaoping Zhang,   UT M.D. Anderson Cancer Center
Strain of Origin(C57BL/6 x DBA/2)F2
Site of ExpressionCol1a2 enhancer drives fibroblast-specific expression of a cre/ERT fusion followed by an IRES-human placental alkaline-phosphatase reporter.
Expressed Gene cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Promoter Col1a2, collagen, type I, alpha 2, mouse, laboratory
Driver Note Col1a2
Inducible Note induced by tamoxifen
Molecular Note The Col1a2 enhancer was used to drive fibroblast-specific expression of a cre/ERT fusion followed by an IRES-human placental alkaline-phosphatase reporter. Two lines were created (lines 7 and 8) and line 7 was used in subsequent analysis. [MGI Ref ID J:134447]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Gt(rosa)26sorTm1Sor, Probe
Tg(Col1a2-cre/ERT)7Cpd, Melt Curve Analysis
Tg(Col1a2-cre/ERT)7Cpd, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zheng B; Zhang Z; Black CM; de Crombrugghe B; Denton CP. 2002. Ligand-dependent genetic recombination in fibroblasts : a potentially powerful technique for investigating gene function in fibrosis. Am J Pathol 160(5):1609-17. [PubMed: 12000713]  [MGI Ref ID J:134447]

Additional References

Tg(Col1a2-cre/ERT)7Cpd related

Blumbach K; Zweers MC; Brunner G; Peters AS; Schmitz M; Schulz JN; Schild A; Denton CP; Sakai T; Fassler R; Krieg T; Eckes B. 2010. Defective granulation tissue formation in mice with specific ablation of integrin-linked kinase in fibroblasts - role of TGFbeta1 levels and RhoA activity. J Cell Sci 123(Pt 22):3872-83. [PubMed: 20980390]  [MGI Ref ID J:182911]

Cai CL; Martin JC; Sun Y; Cui L; Wang L; Ouyang K; Yang L; Bu L; Liang X; Zhang X; Stallcup WB; Denton CP; McCulloch A; Chen J; Evans SM. 2008. A myocardial lineage derives from Tbx18 epicardial cells. Nature 454(7200):104-8. [PubMed: 18480752]  [MGI Ref ID J:137459]

Denton CP; Khan K; Hoyles RK; Shiwen X; Leoni P; Chen Y; Eastwood M; Abraham DJ. 2009. Inducible lineage-specific deletion of TbetaRII in fibroblasts defines a pivotal regulatory role during adult skin wound healing. J Invest Dermatol 129(1):194-204. [PubMed: 18563179]  [MGI Ref ID J:146700]

Duan J; Lee Y; Jania C; Gong J; Rojas M; Burk L; Willis M; Homeister J; Tilley S; Rubin J; Deb A. 2013. Rib fractures and death from deletion of osteoblast betacatenin in adult mice is rescued by corticosteroids. PLoS One 8(2):e55757. [PubMed: 23393600]  [MGI Ref ID J:199426]

Duan W; So T; Mehta AK; Choi H; Croft M. 2011. Inducible CD4+LAP+Foxp3- regulatory T cells suppress allergic inflammation. J Immunol 187(12):6499-507. [PubMed: 22079987]  [MGI Ref ID J:180402]

Hu B; Wu Z; Nakashima T; Phan SH. 2012. Mesenchymal-Specific Deletion of C/EBPbeta Suppresses Pulmonary Fibrosis. Am J Pathol 180(6):2257-67. [PubMed: 22503555]  [MGI Ref ID J:184699]

Ittmann M; Huang J; Radaelli E; Martin P; Signoretti S; Sullivan R; Simons BW; Ward JM; Robinson BD; Chu GC; Loda M; Thomas G; Borowsky A; Cardiff RD. 2013. Animal models of human prostate cancer: the consensus report of the New York meeting of the Mouse Models of Human Cancers Consortium Prostate Pathology Committee. Cancer Res 73(9):2718-36. [PubMed: 23610450]  [MGI Ref ID J:197036]

Jackson RS 2nd; Placzek W; Fernandez A; Ziaee S; Chu CY; Wei J; Stebbins J; Kitada S; Fritz G; Reed JC; Chung LW; Pellecchia M; Bhowmick NA. 2012. Sabutoclax, a Mcl-1 antagonist, inhibits tumorigenesis in transgenic mouse and human xenograft models of prostate cancer. Neoplasia 14(7):656-65. [PubMed: 22904682]  [MGI Ref ID J:204736]

Kapoor M; Liu S; Shi-wen X; Huh K; McCann M; Denton CP; Woodgett JR; Abraham DJ; Leask A. 2008. GSK-3beta in mouse fibroblasts controls wound healing and fibrosis through an endothelin-1-dependent mechanism. J Clin Invest 118(10):3279-90. [PubMed: 18802478]  [MGI Ref ID J:142731]

Liu S; Kapoor M; Leask A. 2009. Rac1 expression by fibroblasts is required for tissue repair in vivo. Am J Pathol 174(5):1847-56. [PubMed: 19349358]  [MGI Ref ID J:147970]

Liu S; Leask A. 2013. Integrin beta1 is required for dermal homeostasis. J Invest Dermatol 133(4):899-906. [PubMed: 23190902]  [MGI Ref ID J:196513]

Liu S; Shi-wen X; Abraham DJ; Leask A. 2011. CCN2 is required for bleomycin-induced skin fibrosis in mice. Arthritis Rheum 63(1):239-46. [PubMed: 20936632]  [MGI Ref ID J:171300]

Liu S; Xu SW; Blumbach K; Eastwood M; Denton CP; Eckes B; Krieg T; Abraham DJ; Leask A. 2010. Expression of integrin beta1 by fibroblasts is required for tissue repair in vivo. J Cell Sci 123(Pt 21):3674-82. [PubMed: 20940256]  [MGI Ref ID J:182925]

Martinez-Ferrer M; Afshar-Sherif AR; Uwamariya C; de Crombrugghe B; Davidson JM; Bhowmick NA. 2010. Dermal transforming growth factor-beta responsiveness mediates wound contraction and epithelial closure. Am J Pathol 176(1):98-107. [PubMed: 19959810]  [MGI Ref ID J:156506]

Parapuram SK; Huh K; Liu S; Leask A. 2011. Integrin beta1 is necessary for the maintenance of corneal structural integrity. Invest Ophthalmol Vis Sci 52(11):7799-806. [PubMed: 21873663]  [MGI Ref ID J:189378]

Parapuram SK; Shi-wen X; Elliott C; Welch ID; Jones H; Baron M; Denton CP; Abraham DJ; Leask A. 2011. Loss of PTEN expression by dermal fibroblasts causes skin fibrosis. J Invest Dermatol 131(10):1996-2003. [PubMed: 21654839]  [MGI Ref ID J:182199]

Riopel MM; Li J; Liu S; Leask A; Wang R. 2013. beta1 integrin-extracellular matrix interactions are essential for maintaining exocrine pancreas architecture and function. Lab Invest 93(1):31-40. [PubMed: 23069938]  [MGI Ref ID J:190701]

Sonnylal S; Denton CP; Zheng B; Keene DR; He R; Adams HP; Vanpelt CS; Geng YJ; Deng JM; Behringer RR; de Crombrugghe B. 2007. Postnatal induction of transforming growth factor beta signaling in fibroblasts of mice recapitulates clinical, histologic, and biochemical features of scleroderma. Arthritis Rheum 56(1):334-44. [PubMed: 17195237]  [MGI Ref ID J:134135]

Zimmermann AS; Morrison SD; Hu MS; Li S; Nauta A; Sorkin M; Meyer NP; Walmsley GG; Maan ZN; Chan DA; Gurtner GC; Giaccia AJ; Longaker MT. 2014. Epidermal or dermal specific knockout of PHD-2 enhances wound healing and minimizes ischemic injury. PLoS One 9(4):e93373. [PubMed: 24695462]  [MGI Ref ID J:215034]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as hemizygotes. The Donating Investigator reports that homozygotes do not breed well.
Mating SystemNoncarrier x Hemizygote         (Female x Male)   18-OCT-11
Hemizygote x Noncarrier         (Female x Male)   18-OCT-11
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $239.00Female or MaleHemizygous for Tg(Col1a2-cre/ERT)7Cpd  
Price per Pair (US dollars $)Pair Genotype
$313.00Hemizygous for Tg(Col1a2-cre/ERT)7Cpd x Noncarrier  
$313.00Noncarrier x Hemizygous for Tg(Col1a2-cre/ERT)7Cpd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $310.70Female or MaleHemizygous for Tg(Col1a2-cre/ERT)7Cpd  
Price per Pair (US dollars $)Pair Genotype
$406.90Hemizygous for Tg(Col1a2-cre/ERT)7Cpd x Noncarrier  
$406.90Noncarrier x Hemizygous for Tg(Col1a2-cre/ERT)7Cpd  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Mice are subject to US Patent 6040430.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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