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| The FOXO1/GFP allele contains a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA) upstream of a forkhead box O1 (Foxo1)-green fluorescent protein (GFP) fusion protein. These mice may be useful for generating conditional mutations for studying the role of PI3K-AKT signaling pathway on insulin resistance, glucose intolerance, diabetes, and aging. | |||||||||||||||
- Description
- Disease & phenotype
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Description
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 28-MAR-12 Species laboratory mouse Donating Investigator Joel K Elmquist, UT Southwestern Med Center at Dallas Description
The FOXO1/GFP allele contains a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA) upstream of a forkhead box O1 (Foxo1)-green fluorescent protein (GFP) fusion protein. Homozygotes are viable and fertile. In the absence of Cre, reporter gene expression is prevented by the STOP sequence. When bred to mice that express Cre recombinase, offspring will have the floxed-STOP cassette deleted in the cre-expressing tissue(s), resulting in FOXO1/GFP expression in Cre-recombinase-expressing cells. FOXO1 is a component of the phosphoinositide 3-kinase (PI3K)-AKT pathway, and shuttles between the nucleus and the cytoplasm upon activation/inhibition by AKT. The PI3K-AKT signaling pathway is activated after administration of insulin and leptin. Exclusion of FoxO1 from the nucleus upon AKT phosphorylation allows visualization of activated/inactivated AKT by monitoring sub-cellular localization of the FOXO1-GFP fusion protein. These mice may be useful for generating conditional mutations for studying the role of PI3K-AKT signaling pathway on insulin resistance, glucose intolerance, diabetes, and aging.Development
The FOXO1/GFP targeting vector was designed with a loxP-flanked transcriptional STOP cassette (PGK-Neo-polyA) upstream of a forkhead box O1 (Foxo1)-green fluorescent protein (GFP) fusion protein. This vector was inserted into the Gt(ROSA)26Sor locus and was electroporated into 129-derived embryonic stem (ES) cells. The resulting chimeric mice were bred to C57BL/6J mice. Upon arrival at The Jackson Laboratory, mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Gt(ROSA)26Sor
View Strains carrying other alleles of Gt(ROSA)26Sor (130 strains)
Phenotype
Phenotype Information
assigned by genotype
Gt(ROSA)26Sortm1(Foxo1/GFP)Jke/Gt(ROSA)26Sor+
involves: 129/Sv * C57BL/6
- no phenotypic analysis
- *normal* no phenotypic analysis
- mice were used to study the dynamics of Foxo1 intracellular localization (MGI Ref ID J:143046)
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Diabetes and Obesity Research
Insulin Resistance
Research Tools
Diabetes and Obesity Research
loxP
Genetics Research
Tissue/Cell Markers: Cre-lox System
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(Foxo1/GFP)Jke | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Joel K Elmquist | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Rosa26-FoxoGFP; | ||
| Mutation Made By | Joel Elmquist, UT Southwestern Med Center at Dallas | ||
| Strain of Origin | 129 | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A floxed neo-STOP cassette followed by a Foxo1/GFP fusion was inserted into the locus. Expression of the reporter construct can only be achieved by cre-mediated removal of the neo-STOP cassette. [MGI Ref ID J:143046] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Gt(ROSA)26Sortm1(Foxo1/GFP)Jke, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Xu F; Ackerley C; Maj MC; Addis JB; Levandovskiy V; Lee J; Mackay N; Cameron JM; Robinson BH. 2008. Disruption of a mitochondrial RNA-binding protein gene results in decreased cytochrome b expression and a marked reduction in ubiquinol-cytochrome c reductase activity in mouse heart mitochondria. Biochem J 416(1):15-26. [PubMed: 18729827] [MGI Ref ID J:143946]
Additional References
Gt(ROSA)26Sortm1(Foxo1/GFP)Jke relatedFukuda M; Jones JE; Olson D; Hill J; Lee CE; Gautron L; Choi M; Zigman JM; Lowell BB; Elmquist JK. 2008. Monitoring FoxO1 localization in chemically identified neurons. J Neurosci 28(50):13640-8. [PubMed: 19074037] [MGI Ref ID J:143046]
Xu Y; Hill JW; Fukuda M; Gautron L; Sohn JW; Kim KW; Lee CE; Choi MJ; Lauzon DA; Dhillon H; Lowell BB; Zigman JM; Zhao JJ; Elmquist JK. 2010. PI3K signaling in the ventromedial hypothalamic nucleus is required for normal energy homeostasis. Cell Metab 12(1):88-95. [PubMed: 20620998] [MGI Ref ID J:163161]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Homozygote x Homozygote (Female x Male) 28-MAR-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(Foxo1/GFP)Jke
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Gt(ROSA)26Sortm1(Foxo1/GFP)Jke x Homozygous for Gt(ROSA)26Sortm1(Foxo1/GFP)Jke Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Gt(ROSA)26Sortm1(Foxo1/GFP)Jke
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Gt(ROSA)26Sortm1(Foxo1/GFP)Jke x Homozygous for Gt(ROSA)26Sortm1(Foxo1/GFP)Jke Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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