Strain Name:

B6.129S6-Ch25htm1Rus/J

Stock Number:

016263

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Availability:

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Homozygous Ch25h (cholesterol 25-hydroxylase) knock-out mice have increased levels of IgA and may be useful in studies innate and adaptive immune system interactions.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   20-JUN-12
Specieslaboratory mouse
GenerationN11+F4 (16-APR-13)
Generation Definitions
 
Donating InvestigatorDr. David Russell,   Univ of Texas Southwest Med Ctr Dallas

Description
Stimulation of toll-like receptors has been shown to normally induce the expression of Ch25h (cholesterol 25-hydroxylase) in macrophages. Il2 (interleukin 2)-mediated stimulation of B cell proliferation is suppressed, activation-induced cytidine deaminase (AID) expression is repressed, and immunoglobulin class switch recombination is blocked, leading to markedly decreased IgA production.

The Ch25h gene was deleted in this targeted mutant strain. Homozygotes have excessive levels of IgA in their sera, mucosa, and the bronchial fluid of the lungs. This strain may be useful in studies of innate immune system regulation of adaptive immune responses.

Development
The intron-less gene was deleted in its entirety and replaced by the PolII neo pA expression cassette in 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. This strain was backcrossed to C57BL/6J for more than 10 generations by the donating laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ch25htm1Rus/Ch25htm1Rus

        B6.129S6-Ch25htm1Rus
  • immune system phenotype
  • increased IgA level
    • in the sera, lungs, and intestinal mucosa   (MGI Ref ID J:170807)
  • increased IgG2a level
    • in the sera, lungs, and intestinal mucosa   (MGI Ref ID J:170807)
  • increased IgG2b level
    • in the intestinal mucosa   (MGI Ref ID J:170807)
  • increased IgG3 level
    • in the intestinal mucosa   (MGI Ref ID J:170807)
  • hematopoietic system phenotype
  • increased IgA level
    • in the sera, lungs, and intestinal mucosa   (MGI Ref ID J:170807)
  • increased IgG2a level
    • in the sera, lungs, and intestinal mucosa   (MGI Ref ID J:170807)
  • increased IgG2b level
    • in the intestinal mucosa   (MGI Ref ID J:170807)
  • increased IgG3 level
    • in the intestinal mucosa   (MGI Ref ID J:170807)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B cell defects
      Macrophage defects
      defects in humoral immune responses

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ch25htm1Rus
Allele Name targeted mutation 1, David W Russell
Allele Type Targeted (Null/Knockout)
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Ch25h, cholesterol 25-hydroxylase
Chromosome 19
Gene Common Name(s) AI462618; C25H; expressed sequence AI462618; m25OH;
Molecular Note The intron-less gene was deleted in its entirety and replaced by the PolII neo pA expression cassette. The absence of protein expression was confirmed by western blot analysis on lung and liver extracts after Kdo2-Lipid A induction. [MGI Ref ID J:170807]

Genotyping

Genotyping Information

Genotyping Protocols

Ch25htm1Rus, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bauman DR; Bitmansour AD; McDonald JG; Thompson BM; Liang G; Russell DW. 2009. 25-Hydroxycholesterol secreted by macrophages in response to Toll-like receptor activation suppresses immunoglobulin A production. Proc Natl Acad Sci U S A 106(39):16764-9. [PubMed: 19805370]  [MGI Ref ID J:170807]

Additional References

Ch25htm1Rus related

Coelho FM; Natale D; Soriano SF; Hons M; Swoger J; Mayer J; Danuser R; Scandella E; Pieczyk M; Zerwes HG; Junt T; Sailer AW; Ludewig B; Sharpe J; Figge MT; Stein JV. 2013. Naive B-cell trafficking is shaped by local chemokine availability and LFA-1-independent stromal interactions. Blood 121(20):4101-9. [PubMed: 23558016]  [MGI Ref ID J:198194]

Gatto D; Wood K; Caminschi I; Murphy-Durland D; Schofield P; Christ D; Karupiah G; Brink R. 2013. The chemotactic receptor EBI2 regulates the homeostasis, localization and immunological function of splenic dendritic cells. Nat Immunol 14(5):446-53. [PubMed: 23502855]  [MGI Ref ID J:196453]

Gold ES; Diercks AH; Podolsky I; Podyminogin RL; Askovich PS; Treuting PM; Aderem A. 2014. 25-Hydroxycholesterol acts as an amplifier of inflammatory signaling. Proc Natl Acad Sci U S A 111(29):10666-71. [PubMed: 24994901]  [MGI Ref ID J:212267]

Hannedouche S; Zhang J; Yi T; Shen W; Nguyen D; Pereira JP; Guerini D; Baumgarten BU; Roggo S; Wen B; Knochenmuss R; Noel S; Gessier F; Kelly LM; Vanek M; Laurent S; Preuss I; Miault C; Christen I; Karuna R; Li W; Koo DI; Suply T; Schmedt C; Peters EC; Falchetto R; Katopodis A; Spanka C; Roy MO; Detheux M; Chen YA; Schultz PG; Cho CY; Seuwen K; Cyster JG; Sailer AW. 2011. Oxysterols direct immune cell migration via EBI2. Nature 475(7357):524-7. [PubMed: 21796212]  [MGI Ref ID J:174682]

Liu SY; Aliyari R; Chikere K; Li G; Marsden MD; Smith JK; Pernet O; Guo H; Nusbaum R; Zack JA; Freiberg AN; Su L; Lee B; Cheng G. 2013. Interferon-inducible cholesterol-25-hydroxylase broadly inhibits viral entry by production of 25-hydroxycholesterol. Immunity 38(1):92-105. [PubMed: 23273844]  [MGI Ref ID J:193039]

Yi T; Cyster JG. 2013. EBI2-mediated bridging channel positioning supports splenic dendritic cell homeostasis and particulate antigen capture. Elife 2:e00757. [PubMed: 23682316]  [MGI Ref ID J:198681]

Yi T; Wang X; Kelly LM; An J; Xu Y; Sailer AW; Gustafsson JA; Russell DW; Cyster JG. 2012. Oxysterol Gradient Generation by Lymphoid Stromal Cells Guides Activated B Cell Movement during Humoral Responses. Immunity 37(3):535-48. [PubMed: 22999953]  [MGI Ref ID J:188276]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX10

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   20-JUN-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Ch25htm1Rus  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Ch25htm1Rus x Homozygous for Ch25htm1Rus  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Ch25htm1Rus  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Ch25htm1Rus x Homozygous for Ch25htm1Rus  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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