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| When combined with a tetO/TRE transgene, this CAG-rtTA mutation enables conditional expression in the presence of doxycycline. This CAGs-rtTA3 transgenic strain is a component of a novel system applying conditional RNA interference (RNAi) to knock out targeted genes. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6N.Cg-Tg(CAG-rtTA3)4288Slowe/J (Changed: 31-MAY-11 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Noncarrier x Hemizygote (Female x Male) 20-OCT-11 Mating System Hemizygote x Noncarrier (Female x Male) 20-OCT-11 Species laboratory mouse Generation N5+F3 (09-JAN-13)
Generation DefinitionsDonating Investigator Dr. Scott Lowe, Cold Spring Harbor Laboratory Description
The CAGs-rtTA3 transgene has a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter driving widespread expression of reverse tetracycline-controlled transactivator 3 (rtTA3). High rtTA3 expression is observed in most tissues examined. Mice hemizygous for the CAGs-rtTA3 transgene are viable and fertile.When crossed with mice carrying a TRE/tetO promoter-directed gene of interest, progeny may be treated with doxycycline to initiate conditional expression.
When crossed with TRE-GFP-shRNAmir (short hairpin microRNA) transgenic mice, the donating laboratory was able to conditionally regulate RNA interference (RNAi) with doxycycline with high efficiency and selectively mark gene knockouts with GFP fluorescence.
Development
A DNA fragment incorporating a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter and highly sensitive reverse tetracycline-controlled transactivator 3 (rtTA3) was introduced to FVB embryos via pronuclear injection. This strain had genetic background contributions from FVB, C57BL/6 and 129. Transgenic mice were subsequently backcrossed to C57BL/6NCrl inbred mice for four generations by the donating laboratory prior to sending to The Jackson Laboratory Repository. Upon arrival, transgenic mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation to establish the live colony. The transgene is integrated on mouse Chromosome 8.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Fluorescent Proteins
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CAG-rtTA3)4288Slowe | ||
|---|---|---|---|
| Allele Name | transgene insertion 4288, Scott Lowe | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | CAGs-rtTA3; | ||
| Strain of Origin | FVB | ||
| Site of Expression | rtTA3 expression is observed in most tissues. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | CMV, cytomegalovirus, human | ||
| Molecular Note | The transgene contains a DNA fragment incorporating a CAG (cytomegalovirus (CMV) early enhancer element and chicken beta-actin) promoter and highly sensitive reverse tetracycline-controlled transactivator 3 (rtTA3). Line 4288 was generated. [MGI Ref ID J:171191] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(CAG-rtTA3)4288Slowe, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Premsrirut PK; Dow LE; Kim SY; Camiolo M; Malone CD; Miething C; Scuoppo C; Zuber J; Dickins RA; Kogan SC; Shroyer KR; Sordella R; Hannon GJ; Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145(1):145-58. [PubMed: 21458673] [MGI Ref ID J:171191]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintained as a live colony, hemizygous mice may be bred with wildtype (noncarrier) mice from the colony or with C57BL/6NJ inbred mice (Stock No. 005304). Mating System Noncarrier x Hemizygote (Female x Male) 20-OCT-11 Hemizygote x Noncarrier (Female x Male) 20-OCT-11
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(CAG-rtTA3)4288Slowe
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(CAG-rtTA3)4288Slowe x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(CAG-rtTA3)4288Slowe Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(CAG-rtTA3)4288Slowe
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(CAG-rtTA3)4288Slowe x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(CAG-rtTA3)4288Slowe Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 005304 C57BL/6NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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