Strain Name:

FVB-Tg(Myh6-TRPC3*)6.6Jmol/J

Stock Number:

016570

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
The dnTRPC3 transgene contains a cardiac specific dominant negative human TRPC3 gene. These mice may be useful for studying the role plyed by TRPC in calcineurin signaling and myocardial growth.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB-Tg(Myh6-TRPC3)6.6Jmol/J    (Changed: 01-JUN-11 )
Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Jeffery D. Molkentin,   Cincinnati Children's Hospital

Description
The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) gene under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Hemizygotes are viable, fertile and normal in size. TRPC3 is a nonselective monovalent cation channel which is activated by oxidative stress in endothelial cells. These mice express a dominant-negative form of TRPC3. They only express the N-terminus of the gene and lack the transmembrane and cytosolic carboxy terminal domains. TRPC3 transgenic mice exhibit an increase in cardiac calcineurin-nuclear factor of activated T cells (NFAT) transcriptional activity, cardiac hypertrophy, and cardiomyopathy. They show a dosage-dependent increase in cardiac hypertrophy following neuro-endocrine agonist or pressure overload stimulation.

Development
The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. The construct was microinjected into fertilized FVB/N oocytes, and offspring from founder line 6.6 were bred to FVB/N mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myh6
012389   B6.Cg-Tg(Myh6-Ppara)404-3Dpk/J
012382   B6.Cg-Tg(Myh6-Ppara)404-4Dpk/J
005657   B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J
011038   B6.FVB-Tg(Myh6-cre)2182Mds/J
013781   B6;FVB-Tg(Myh6/NFAT-luc)1Jmol/J
002640   B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
012383   B6CBA-Tg(Myh6-Ppard)HEDpk/J
018972   B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J
002639   B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
002638   B6SJL-Tg(WTbeta2)4Wjk/J
006768   D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ
010587   FVB-Tg(Myh6-MEF2A)1Jmol/J
010581   FVB-Tg(Myh6-Map2k1*)1Jmol/J
010582   FVB-Tg(Myh6-Map2k3*)1Jmol/J
010584   FVB-Tg(Myh6-Map2k7)1Jmol/J
010585   FVB-Tg(Myh6-Mapk1)1Jmol/J
010583   FVB-Tg(Myh6-Mapk14*)1Jmol/J
010586   FVB-Tg(Myh6-Mef2c)2Jmol/J
009438   FVB-Tg(Myh6-SOD2,Tyr)3Pne/J
017543   FVB-Tg(Myh6-TRPC6*)1Jmol/J
011037   FVB-Tg(Myh6-cre)2182Mds/J
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
014153   FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J
012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
010580   FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J
022979   FVB-Tg(tetO-Thbs4)17.7Jmol/J
002535   FVB/N-Tg(ANX6)2Agh/J
012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
008716   FVB/N-Tg(Myh6-AIP/PLN*)46Jded/J
012461   FVB/N-Tg(Myh6-Cast)1Gwd/J
012460   FVB/N-Tg(Myh6-Gnaq)40Gwd/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
009075   STOCK Tg(Myh6-Ppp3ca)37Eno/J
010579   STOCK Tg(Myh6-Prkca)1Jmk/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
005650   STOCK Tg(Myh6-cre/Esr1*)1Jmk/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
View Strains carrying other alleles of Myh6     (40 strains)

Strains carrying other alleles of TRPC3
024052   FVB-Tg(ACTA1*-TRPC3)1Jmol/J
View Strains carrying other alleles of TRPC3     (1 strain)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities
      cardiomyopathy

Cell Biology Research
Channel and Transporter Defects
      calcium
Transcriptional Regulation

Developmental Biology Research
Internal/Organ Defects
      heart

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Myh6-TRPC3*)6.6Jmol
Allele Name transgene insertion 6.6, Jeffery Molkentin
Allele Type Transgenic (random, expressed)
Common Name(s) dnTRPC3;
Strain of OriginFVB
Expressed Gene TRPC3, transient receptor potential cation channel, subfamily C, member 3, human
Promoter Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine
Molecular Note The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Line 6.6 was generated. [MGI Ref ID J:159297]
 
 

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wu X; Eder P; Chang B; Molkentin JD. 2010. TRPC channels are necessary mediators of pathologic cardiac hypertrophy. Proc Natl Acad Sci U S A 107(15):7000-5. [PubMed: 20351294]  [MGI Ref ID J:159297]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice may be crossed to wildtype (non-carrier) mice from the colony or to FVB/NJ inbred mice (Stock No. 001800).

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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