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| The dnTRPC3 transgene contains a cardiac specific dominant negative human TRPC3 gene. These mice may be useful for studying the role plyed by TRPC in calcineurin signaling and myocardial growth. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names FVB-Tg(Myh6-TRPC3)6.6Jmol/J (Changed: 01-JUN-11 ) Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1
Generation DefinitionsDonating Investigator Jeffery D. Molkentin, Children's Hospital Medical Center Description
The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) gene under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Hemizygotes are viable, fertile and normal in size. TRPC3 is a nonselective monovalent cation channel which is activated by oxidative stress in endothelial cells. These mice express a dominant-negative form of TRPC3. They only express the N-terminus of the gene and lack the transmembrane and cytosolic carboxy terminal domains. TRPC3 transgenic mice exhibit an increase in cardiac calcineurin-nuclear factor of activated T cells (NFAT) transcriptional activity, cardiac hypertrophy, and cardiomyopathy. They show a dosage-dependent increase in cardiac hypertrophy following neuro-endocrine agonist or pressure overload stimulation.Development
The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. The construct was microinjected into fertilized FVB/N oocytes, and offspring from founder line 6.6 were bred to FVB/N mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Myh6
View Strains carrying other alleles of Myh6 (38 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cardiovascular Research
Heart Abnormalities
cardiomyopathy
Cell Biology Research
Channel and Transporter Defects
calcium
Transcriptional Regulation
Developmental Biology Research
Internal/Organ Defects
heart
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Myh6-TRPC3*)6.6Jmol | ||
|---|---|---|---|
| Allele Name | transgene insertion 6.6, Jeffery Molkentin | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | dnTRPC3; | ||
| Strain of Origin | FVB | ||
| Expressed Gene | TRPC3, transient receptor potential cation channel, subfamily C, member 3, human | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine | ||
| Molecular Note | The dnTRPC3 transgene contains amino acids 1-302 of the human transient receptor potential cation channel, subfamily C, member 3 (TRPC3) under the control of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Line 6.6 was generated. [MGI Ref ID J:159297] | ||
References
References provided by MGI
Selected Reference(s)
Wu X; Eder P; Chang B; Molkentin JD. 2010. TRPC channels are necessary mediators of pathologic cardiac hypertrophy. Proc Natl Acad Sci U S A 107(15):7000-5. [PubMed: 20351294] [MGI Ref ID J:159297]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be crossed to wildtype (non-carrier) mice from the colony or to FVB/NJ inbred mice (Stock No. 001800).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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