Strain Name:

B6.129(FVB)-Igf1tm1Dlr/J

Stock Number:

016831

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Availability:

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These floxed mutant mice possess loxP sites flanking exon 4 of the Igf1 gene and a neomycin resistance (neo) cassette. This strain may be useful for studying postnatal growth and development.

Description

Strain Information

Former Names B6.Cg-Igf1tm1Dlr/J    (Changed: 14-JUN-11 )
Type Congenic; Mutant Stock; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   17-SEP-12
Specieslaboratory mouse
GenerationN10+f4 (27-DEC-11)
Generation Definitions
 
Donating InvestigatorDr. Derek LeRoith,   Mount Sinai School of Medicine

Description
These mutant mice feature loxP sites flanking exon 4 of the insulin-like growth factor 1 (Igf1) gene and a neomycin resistance cassette. Homozygotes for this allele are viable, fertile, and normal in size. IGF1 promotes cell survival and cell proliferation by activating the tyrosine kinase transmembrane receptor, IGF-1R. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 4 deleted in cre-expressing tissues. For example, when these mice are bred to Tg(Alb1-cre)1Dlr/J mice (Stock No. 016832 or Stock No. 016833), IGF1 expression is abolished in liver. This strain may be useful for studying postnatal growth and development.

When bred to a strain expressing Cre recombinase in the early mouse embryo (see Stock No. 003724 for example), this mutant mouse strain may be useful in studies of early development.

Development
A targeting vector was designed to insert a loxP site upstream of exon 4 and a neomycin resistance (neo) cassette and second loxP site downstream of exon 4 of the insulin-like growth factor 1 (Igf1) gene. The construct was electroporated into 129-derived embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were bred with C57BL/6J females, and their offspring were subsequently crossed to FVB-Tg(Alb1-cre)1Dlr mice. The donating investigator reported that these mice were backcrossed to C57BL/6J inbred mice for at least 9 generations (see SNP note below). Upon arrival, the mice were bred to C57BL/6J inbred mice (Stock No. 000664) for one generation and transgene was selectively bred out of the line.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Igf1tm1Dlr allele
012663   FVB.129(B6)-Igf1tm1Dlr/J
View Strains carrying   Igf1tm1Dlr     (1 strain)

Strains carrying other alleles of Igf1
003258   STOCK Igf1tm1Ts/ImJ
003259   STOCK Igf1tm2Ts/ImJ
View Strains carrying other alleles of Igf1     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Insulin-Like Growth Factor I Deficiency   (IGF1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Igf1tm1Dlr/Igf1tm1Dlr Tg(Alb1-cre)1Dlr/0

        involves: 129/Sv * C57BL/6 * FVB/N   (conditional)
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:69517)

Igf1tm1Dlr/Igf1tm1Dlr Tg(Alb1-cre)1Dlr/0

        involves: 129/Sv * C57BL/6J * FVB/N   (conditional)
  • homeostasis/metabolism phenotype
  • decreased circulating insulin-like growth factor I level
    • male mutant LID (LID, chronic liver-specific IGF-1 deficiency) mice fed a high calorie or regular diet for 8 to 10 weeks exhibit decreased serum IGF-1 levels relative to controls   (MGI Ref ID J:155733)
  • increased circulating leptin level
    • LID mice fed a high calorie for 8 to 10 weeks exhibit increased serum leptin levels compared to lean LID mice (fed a regular diet)   (MGI Ref ID J:155733)
  • tumorigenesis
  • decreased tumor growth/size
    • male double mutant (LID, chronic liver-specific IGF-1 deficiency) mice on a high calorie diet for 10-14 weeks and injected with subcutaneous MC-39 murine colorectal carcinoma cell do not show the obesity-associated increase in local tumor growth compared to lean controls   (MGI Ref ID J:155733)

Igf1tm1Dlr/Igf1tm1Dlr Tg(EIIa-cre)C5379Lmgd/0

        involves: 129/Sv * C57BL/6 * FVB/N   (conditional)
  • mortality/aging
  • partial perinatal lethality
    • some perinatal mortality but many survive   (MGI Ref ID J:49468)
  • growth/size/body phenotype
  • fetal growth retardation
    • fetus at E19 or E20 weighs only 69% as much as comparable controls   (MGI Ref ID J:49468)
  • slow postnatal weight gain
    • 72% of control weight at 3 weeks of age, 68% at 6 weeks   (MGI Ref ID J:49468)

Igf1tm1Dlr/Igf1tm1Dlr Tg(Mx1-cre)1Cgn/0

        involves: 129/Sv * C57BL/6 * CBA   (conditional)
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • after induction with PiPc, 6-mo-old mice exhibit no significant differences in serum and urine creatinine levels relative to control mice   (MGI Ref ID J:121798)
    • decreased circulating insulin-like growth factor I level
      • after induction with IFN at 24-28 days of age, mice exhibit a 75% reduction in serum IGF-I levels relative to control mice, still evident at 53 days of age   (MGI Ref ID J:55718)
      • after induction with PiPc at 4 weeks of age, mice exhibit a 80-85% reduction in serum IGF-I levels relative to control mice, still evident at 24 months of age   (MGI Ref ID J:121798)
      • renal IGF-II mRNA levels are reduced by 79%, suggesting that the effects of circulating IGF-I on kidney size may be mediated by renal IGF-II; however, no differences in serum IGF-II levels are observed at 9 months   (MGI Ref ID J:121798)
    • increased circulating growth hormone level
      • after induction with PiPc, mice exhibit compensatory increased circulating GH levels relative to control mice   (MGI Ref ID J:121798)
    • increased urine potassium level
      • after induction with PiPc, 6-mo-old mice exhibit increased 24-hr potassium excretion relative to control mice   (MGI Ref ID J:121798)
    • increased urine sodium level
      • after induction with PiPc, 6-mo-old mice exhibit increased 24-hr sodium excretion relative to control mice   (MGI Ref ID J:121798)
  • growth/size/body phenotype
  • *normal* growth/size/body phenotype
    • after induction with IFN at 24-28 days of age, mice exhibit normal postnatal body growth up to 53 days of age relative to control mice   (MGI Ref ID J:55718)
    • after induction with PiPc at 4 weeks of age, mice exhibit no significant differences in body weight relative to control mice, as shown at 7 and 24 months of age   (MGI Ref ID J:121798)
  • renal/urinary system phenotype
  • *normal* renal/urinary system phenotype
    • after induction with PiPc, 6-mo-old mice exhibit no significant differences in serum and urine creatinine levels, GFR, creatinine clearance, or 24-hr urine volume relative to control mice   (MGI Ref ID J:121798)
    • no glomerulosclerosis, vascular defects, mesangial sclerosis, or cortical fibrosis is observed at 24 months of age   (MGI Ref ID J:121798)
    • decreased kidney weight
      • after induction with PiPc, absolute and relative kidney weight is reduced in 7- and 24-month-old mice relative to control mice   (MGI Ref ID J:121798)
    • increased urine potassium level
      • after induction with PiPc, 6-mo-old mice exhibit increased 24-hr potassium excretion relative to control mice   (MGI Ref ID J:121798)
    • increased urine sodium level
      • after induction with PiPc, 6-mo-old mice exhibit increased 24-hr sodium excretion relative to control mice   (MGI Ref ID J:121798)
  • cardiovascular system phenotype
  • increased systemic arterial systolic blood pressure
    • after induction with PiPc, 7-mo-old mice exhibit increased systolic blood pressure relative to control mice   (MGI Ref ID J:121798)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences
Diabetes and Obesity Research
      loxP

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Igf1tm1Dlr
Allele Name targeted mutation 1, Derek LeRoith
Allele Type Targeted (Floxed/Frt)
Common Name(s) IGF-I loxP; IGF-Iflox; LI-IGF-I-; LID;
Mutation Made ByDr. Derek LeRoith,   Mount Sinai School of Medicine
Strain of Origin129
Gene Symbol and Name Igf1, insulin-like growth factor 1
Chromosome 10
Gene Common Name(s) C730016P09Rik; IGF-I; IGF1A; IGFI; Igf-1; RIKEN cDNA C730016P09 gene;
General Note ES cells = 129/Sv
Molecular Note A neomycin cassette and loxP sites were inserted flanking exon 4. The introduction of these mutations had no effect on the normal function of the gene. [MGI Ref ID J:49468]

Genotyping

Genotyping Information

Genotyping Protocols

Igf1tm1Dlr, Melt Curve Analysis
Igf1tm1Dlr, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Liu JL; Grinberg A; Westphal H; Sauer B; Accili D; Karas M; LeRoith D. 1998. Insulin-like growth factor-I affects perinatal lethality and postnatal development in a gene dosage-dependent manner: manipulation using the Cre/loxP system in transgenic mice. Mol Endocrinol 12(9):1452-62. [PubMed: 9731712]  [MGI Ref ID J:49468]

Additional References

Igf1tm1Dlr related

Anzo M; Cobb LJ; Hwang DL; Mehta H; Said JW; Yakar S; LeRoith D; Cohen P. 2008. Targeted deletion of hepatic Igf1 in TRAMP mice leads to dramatic alterations in the circulating insulin-like growth factor axis but does not reduce tumor progression. Cancer Res 68(9):3342-9. [PubMed: 18451161]  [MGI Ref ID J:134610]

Bailey-Downs LC; Mitschelen M; Sosnowska D; Toth P; Pinto JT; Ballabh P; Valcarcel-Ares MN; Farley J; Koller A; Henthorn JC; Bass C; Sonntag WE; Ungvari Z; Csiszar A. 2012. Liver-specific knockdown of IGF-1 decreases vascular oxidative stress resistance by impairing the Nrf2-dependent antioxidant response: a novel model of vascular aging. J Gerontol A Biol Sci Med Sci 67(4):313-29. [PubMed: 22021391]  [MGI Ref ID J:190195]

Cochrane RL; Clark SH; Harris A; Kream BE. 2007. Rearrangement of a conditional allele regardless of inheritance of a Cre recombinase transgene. Genesis 45(1):17-20. [PubMed: 17211878]  [MGI Ref ID J:125041]

Cooper KL; Oh S; Sung Y; Dasari RR; Kirschner MW; Tabin CJ. 2013. Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions. Nature 495(7441):375-8. [PubMed: 23485973]  [MGI Ref ID J:195127]

Courtland HW; Elis S; Wu Y; Sun H; Rosen CJ; Jepsen KJ; Yakar S. 2011. Serum IGF-1 affects skeletal acquisition in a temporal and compartment-specific manner. PLoS One 6(3):e14762. [PubMed: 21445249]  [MGI Ref ID J:171681]

Della Torre S; Rando G; Meda C; Stell A; Chambon P; Krust A; Ibarra C; Magni P; Ciana P; Maggi A. 2011. Amino acid-dependent activation of liver estrogen receptor alpha integrates metabolic and reproductive functions via IGF-1. Cell Metab 13(2):205-14. [PubMed: 21284987]  [MGI Ref ID J:169561]

Ealey KN; Xuan W; Lu S; Archer MC. 2008. Colon carcinogenesis in liver-specific IGF-I-deficient (LID) mice. Int J Cancer 122(2):472-6. [PubMed: 17918153]  [MGI Ref ID J:135544]

Endres M; Piriz J; Gertz K; Harms C; Meisel A; Kronenberg G; Torres-Aleman I. 2007. Serum insulin-like growth factor I and ischemic brain injury. Brain Res 1185:328-35. [PubMed: 17961524]  [MGI Ref ID J:128923]

Fernandez MC; Venara M; Nowicki S; Chemes HE; Barontini M; Pennisi PA. 2012. Igf-I regulates pheochromocytoma cell proliferation and survival in vitro and in vivo. Endocrinology 153(8):3724-34. [PubMed: 22653556]  [MGI Ref ID J:189111]

Fritton JC; Emerton KB; Sun H; Kawashima Y; Mejia W; Wu Y; Rosen CJ; Panus D; Bouxsein M; Majeska RJ; Schaffler MB; Yakar S. 2010. Growth hormone protects against ovariectomy-induced bone loss in states of low circulating insulin-like growth factor (IGF-1). J Bone Miner Res 25(2):235-46. [PubMed: 19619004]  [MGI Ref ID J:179871]

Govoni KE; Lee SK; Chung YS; Behringer RR; Wergedal JE; Baylink DJ; Mohan S. 2007. Disruption of insulin-like growth factor-I expression in type IIalphaI collagen-expressing cells reduces bone length and width in mice. Physiol Genomics 30(3):354-62. [PubMed: 17519362]  [MGI Ref ID J:127222]

Govoni KE; Wergedal JE; Florin L; Angel P; Baylink DJ; Mohan S. 2007. Conditional deletion of insulin-like growth factor-I in collagen type 1alpha2-expressing cells results in postnatal lethality and a dramatic reduction in bone accretion. Endocrinology 148(12):5706-15. [PubMed: 17717052]  [MGI Ref ID J:131108]

Haluzik M; Yakar S; Gavrilova O; Setser J; Boisclair Y; LeRoith D. 2003. Insulin resistance in the liver-specific IGF-1 gene-deleted mouse is abrogated by deletion of the acid-labile subunit of the IGF-binding protein-3 complex: relative roles of growth hormone and IGF-1 in insulin resistance. Diabetes 52(10):2483-9. [PubMed: 14514630]  [MGI Ref ID J:107186]

Kesavan C; Wergedal JE; Lau KH; Mohan S. 2011. Conditional disruption of IGF-I gene in type 1alpha collagen-expressing cells shows an essential role of IGF-I in skeletal anabolic response to loading. Am J Physiol Endocrinol Metab 301(6):E1191-7. [PubMed: 21878662]  [MGI Ref ID J:182168]

Lau KH; Baylink DJ; Zhou XD; Rodriguez D; Bonewald LF; Li Z; Ruffoni D; Muller R; Kesavan C; Sheng MH. 2013. Osteocyte-derived insulin-like growth factor I is essential for determining bone mechanosensitivity. Am J Physiol Endocrinol Metab 305(2):E271-81. [PubMed: 23715728]  [MGI Ref ID J:199255]

Lee C; Safdie FM; Raffaghello L; Wei M; Madia F; Parrella E; Hwang D; Cohen P; Bianchi G; Longo VD. 2010. Reduced levels of IGF-I mediate differential protection of normal and cancer cells in response to fasting and improve chemotherapeutic index. Cancer Res 70(4):1564-72. [PubMed: 20145127]  [MGI Ref ID J:157609]

Lindberg MK; Svensson J; Venken K; Chavoshi T; Andersson N; Moverare Skrtic S; Isaksson O; Vanderschueren D; Carlsten H; Ohlsson C. 2006. Liver-derived IGF-I is permissive for ovariectomy-induced trabecular bone loss. Bone 38(1):85-92. [PubMed: 16257281]  [MGI Ref ID J:123447]

Liu JL; LeRoith D. 1999. Insulin-like growth factor I is essential for postnatal growth in response to growth hormone. Endocrinology 140(11):5178-84. [PubMed: 10537147]  [MGI Ref ID J:69518]

Liu JL; Yakar S; LeRoith D. 2000. Mice deficient in liver production of insulin-like growth factor I display sexual dimorphism in growth hormone-stimulated postnatal growth Endocrinology 141(12):4436-41. [PubMed: 11108252]  [MGI Ref ID J:66082]

Liu YL; Yakar S; Otero-Corchon V; Low MJ; Liu JL. 2002. Ghrelin gene expression is age-dependent and influenced by gender and the level of circulating IGF-I. Mol Cell Endocrinol 189(1-2):97-103. [PubMed: 12039068]  [MGI Ref ID J:76060]

Loladze AV; Stull MA; Rowzee AM; Demarco J; Lantry JH 3rd; Rosen CJ; Leroith D; Wagner KU; Hennighausen L; Wood TL. 2006. Epithelial-specific and stage-specific functions of insulin-like growth factor-I during postnatal mammary development. Endocrinology 147(11):5412-23. [PubMed: 16901968]  [MGI Ref ID J:117180]

Lopez-Lopez C; LeRoith D; Torres-Aleman I. 2004. Insulin-like growth factor I is required for vessel remodeling in the adult brain. Proc Natl Acad Sci U S A 101(26):9833-8. [PubMed: 15210967]  [MGI Ref ID J:91431]

Lu Y; Herrera PL; Guo Y; Sun D; Tang Z; LeRoith D; Liu JL. 2004. Pancreatic-specific inactivation of IGF-I gene causes enlarged pancreatic islets and significant resistance to diabetes. Diabetes 53(12):3131-41. [PubMed: 15561943]  [MGI Ref ID J:94578]

Matheny RW; Merritt E; Zannikos SV; Farrar RP; Adamo ML. 2009. Serum IGF-I-deficiency does not prevent compensatory skeletal muscle hypertrophy in resistance exercise. Exp Biol Med (Maywood) 234(2):164-70. [PubMed: 19064939]  [MGI Ref ID J:165003]

Menagh PJ; Turner RT; Jump DB; Wong CP; Lowry MB; Yakar S; Rosen CJ; Iwaniec UT. 2010. Growth hormone regulates the balance between bone formation and bone marrow adiposity. J Bone Miner Res 25(4):757-68. [PubMed: 19821771]  [MGI Ref ID J:179868]

Mitschelen M; Yan H; Farley JA; Warrington JP; Han S; Herenu CB; Csiszar A; Ungvari Z; Bailey-Downs LC; Bass CE; Sonntag WE. 2011. Long-term deficiency of circulating and hippocampal insulin-like growth factor I induces depressive behavior in adult mice: a potential model of geriatric depression. Neuroscience 185:50-60. [PubMed: 21524689]  [MGI Ref ID J:173929]

Moore T; Carbajal S; Beltran L; Perkins SN; Yakar S; Leroith D; Hursting SD; Digiovanni J. 2008. Reduced susceptibility to two-stage skin carcinogenesis in mice with low circulating insulin-like growth factor I levels. Cancer Res 68(10):3680-8. [PubMed: 18483250]  [MGI Ref ID J:135023]

Naranjo WM; Yakar S; Sanchez-Gomez M; Perez AU; Setser J; LERoith D. 2002. Protein calorie restriction affects nonhepatic IGF-I production and the lymphoid system: studies using the liver-specific IGF-I gene-deleted mouse model. Endocrinology 143(6):2233-41. [PubMed: 12021187]  [MGI Ref ID J:77528]

Niu T; Rosen CJ. 2005. The insulin-like growth factor-I gene and osteoporosis: a critical appraisal. Gene 361:38-56. [PubMed: 16183214]  [MGI Ref ID J:102683]

Pennisi PA; Kopchick JJ; Thorgeirsson S; LeRoith D; Yakar S. 2004. Role of growth hormone (GH) in liver regeneration. Endocrinology 145(10):4748-55. [PubMed: 15242989]  [MGI Ref ID J:135391]

Sheng MH; Zhou XD; Bonewald LF; Baylink DJ; Lau KH. 2013. Disruption of the insulin-like growth factor-1 gene in osteocytes impairs developmental bone growth in mice. Bone 52(1):133-44. [PubMed: 23032105]  [MGI Ref ID J:193782]

Sjogren K; Liu JL; Blad K; Skrtic S; Vidal O; Wallenius V; LeRoith D; Tornell J; Isaksson OG; Jansson JO; Ohlsson C. 1999. Liver-derived insulin-like growth factor I (IGF-I) is the principal source of IGF-I in blood but is not required for postnatal body growth in mice. Proc Natl Acad Sci U S A 96(12):7088-92. [PubMed: 10359843]  [MGI Ref ID J:55718]

Sjogren K; Sheng M; Moverare S; Liu JL; Wallenius K; Tornell J; Isaksson O; Jansson JO; Mohan S; Ohlsson C. 2002. Effects of liver-derived insulin-like growth factor I on bone metabolism in mice. J Bone Miner Res 17(11):1977-87. [PubMed: 12412805]  [MGI Ref ID J:111425]

Svensson J; Diez M; Engel J; Wass C; Tivesten A; Jansson JO; Isaksson O; Archer T; Hokfelt T; Ohlsson C. 2006. Endocrine, liver-derived IGF-I is of importance for spatial learning and memory in old mice. J Endocrinol 189(3):617-27. [PubMed: 16731792]  [MGI Ref ID J:109081]

Svensson J; Kindblom J; Shao R; Moverare-Skrtic S; Lagerquist MK; Andersson N; Sjogren K; Venken K; Vanderschueren D; Jansson JO; Isaksson O; Ohlsson C. 2008. Liver-derived IGF1 enhances the androgenic response in prostate. J Endocrinol 199(3):489-97. [PubMed: 18827067]  [MGI Ref ID J:142809]

Svensson J; Sjogren K; Faldt J; Andersson N; Isaksson O; Jansson JO; Ohlsson C. 2011. Liver-derived IGF-I regulates mean life span in mice. PLoS One 6(7):e22640. [PubMed: 21799924]  [MGI Ref ID J:175763]

Svensson J; Soderpalm B; Sjogren K; Engel J; Ohlsson C. 2005. Liver-derived IGF-I regulates exploratory activity in old mice. Am J Physiol Endocrinol Metab 289(3):E466-73. [PubMed: 15840636]  [MGI Ref ID J:100921]

Svensson J; Tivesten A; Sjogren K; Isaksson O; Bergstrom G; Mohan S; Molne J; Isgaard J; Ohlsson C. 2007. Liver-derived IGF-I regulates kidney size, sodium reabsorption, and renal IGF-II expression. J Endocrinol 193(3):359-66. [PubMed: 17535874]  [MGI Ref ID J:121798]

Tang Z; Yu R; Lu Y; Parlow AF; Liu JL. 2005. Age-dependent onset of liver-specific IGF-I gene deficiency and its persistence in old age: implications for postnatal growth and insulin resistance in LID mice. Am J Physiol Endocrinol Metab 289(2):E288-95. [PubMed: 15769793]  [MGI Ref ID J:100319]

Temmerman L; Slonimsky E; Rosenthal N. 2010. Class 2 IGF-1 isoforms are dispensable for viability, growth and maintenance of IGF-1 serum levels. Growth Horm IGF Res :. [PubMed: 20382057]  [MGI Ref ID J:159167]

Tivesten A; Bollano E; Andersson I; Fitzgerald S; Caidahl K; Sjogren K; Skott O; Liu JL; Mobini R; Isaksson OG; Jansson JO; Ohlsson C; Bergstrom G; Isgaard J. 2002. Liver-derived insulin-like growth factor-I is involved in the regulation of blood pressure in mice. Endocrinology 143(11):4235-42. [PubMed: 12399417]  [MGI Ref ID J:80468]

Trejo JL; Llorens-Martin MV; Torres-Aleman I. 2008. The effects of exercise on spatial learning and anxiety-like behavior are mediated by an IGF-I-dependent mechanism related to hippocampal neurogenesis. Mol Cell Neurosci 37(2):402-11. [PubMed: 18086533]  [MGI Ref ID J:132686]

Trejo JL; Piriz J; Llorens-Martin MV; Fernandez AM; Bolos M; LeRoith D; Nunez A; Torres-Aleman I. 2007. Central actions of liver-derived insulin-like growth factor I underlying its pro-cognitive effects. Mol Psychiatry 12(12):1118-28. [PubMed: 17848918]  [MGI Ref ID J:147526]

Troncoso R; Vicencio JM; Parra V; Nemchenko A; Kawashima Y; Del Campo A; Toro B; Battiprolu PK; Aranguiz P; Chiong M; Yakar S; Gillette TG; Hill JA; Abel ED; Leroith D; Lavandero S. 2012. Energy-preserving effects of IGF-1 antagonize starvation-induced cardiac autophagy. Cardiovasc Res 93(2):320-9. [PubMed: 22135164]  [MGI Ref ID J:194869]

Villa A; Della Torre S; Stell A; Cook J; Brown M; Maggi A. 2012. Tetradian oscillation of estrogen receptor alpha is necessary to prevent liver lipid deposition. Proc Natl Acad Sci U S A 109(29):11806-11. [PubMed: 22761311]  [MGI Ref ID J:186489]

Wallenius K; Sjogren K; Peng XD; Park S; Wallenius V; Liu JL; Umaerus M; Wennbo H; Isaksson O; Frohman L; Kineman R; Ohlsson C; Jansson JO. 2001. Liver-derived igf-i regulates gh secretion at the pituitary level in mice. Endocrinology 142(11):4762-70. [PubMed: 11606442]  [MGI Ref ID J:72793]

Welniak LA; Karas M; Yakar S; Anver MR; Murphy WJ; LeRoith D. 2004. Effects of organ-specific loss of insulin-like growth factor-I production on murine hematopoiesis. Biol Blood Marrow Transplant 10(1):32-9. [PubMed: 14752777]  [MGI Ref ID J:109504]

Wu Y; Brodt P; Sun H; Mejia W; Novosyadlyy R; Nunez N; Chen X; Mendoza A; Hong SH; Khanna C; Yakar S. 2010. Insulin-like growth factor-I regulates the liver microenvironment in obese mice and promotes liver metastasis. Cancer Res 70(1):57-67. [PubMed: 20048072]  [MGI Ref ID J:155733]

Xian L; Wu X; Pang L; Lou M; Rosen CJ; Qiu T; Crane J; Frassica F; Zhang L; Rodriguez JP; Xiaofeng Jia; Shoshana Yakar; Shouhong Xuan; Argiris Efstratiadis; Mei Wan; Xu Cao. 2012. Matrix IGF-1 maintains bone mass by activation of mTOR in mesenchymal stem cells. Nat Med 18(7):1095-101. [PubMed: 22729283]  [MGI Ref ID J:197585]

Yakar S; Bouxsein ML; Canalis E; Sun H; Glatt V; Gundberg C; Cohen P; Hwang D; Boisclair Y; Leroith D; Rosen CJ. 2006. The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone. J Endocrinol 189(2):289-99. [PubMed: 16648296]  [MGI Ref ID J:108341]

Yakar S; Canalis E; Sun H; Mejia W; Kawashima Y; Nasser P; Courtland HW; Williams V; Bouxsein M; Rosen C; Jepsen KJ. 2009. Serum IGF-1 determines skeletal strength by regulating subperiosteal expansion and trait interactions. J Bone Miner Res 24(8):1481-92. [PubMed: 19257833]  [MGI Ref ID J:168668]

Yakar S; Liu JL; Fernandez AM; Wu Y; Schally AV; Frystyk J; Chernausek SD; Mejia W; Le Roith D. 2001. Liver-specific igf-1 gene deletion leads to muscle insulin insensitivity. Diabetes 50(5):1110-8. [PubMed: 11334415]  [MGI Ref ID J:68986]

Yakar S; Liu JL; Stannard B; Butler A; Accili D; Sauer B; LeRoith D. 1999. Normal growth and development in the absence of hepatic insulin-like growth factor I. Proc Natl Acad Sci U S A 96(13):7324-9. [PubMed: 10377413]  [MGI Ref ID J:69517]

Yakar S; Rosen CJ; Beamer WG; Ackert-Bicknell CL; Wu Y; Liu JL; Ooi GT; Setser J; Frystyk J; Boisclair YR; LeRoith D. 2002. Circulating levels of IGF-1 directly regulate bone growth and density. J Clin Invest 110(6):771-81. [PubMed: 12235108]  [MGI Ref ID J:79109]

Yakar S; Rosen CJ; Bouxsein ML; Sun H; Mejia W; Kawashima Y; Wu Y; Emerton K; Williams V; Jepsen K; Schaffler MB; Majeska RJ; Gavrilova O; Gutierrez M; Hwang D; Pennisi P; Frystyk J; Boisclair Y; Pintar J; Jasper H; Domene H; Cohen P; Clemmons D; LeRoithD. 2009. Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism. FASEB J 23(3):709-19. [PubMed: 18952711]  [MGI Ref ID J:146019]

Yakar S; Setser J; Zhao H; Stannard B; Haluzik M; Glatt V; Bouxsein ML; Kopchick JJ; LeRoith D. 2004. Inhibition of growth hormone action improves insulin sensitivity in liver IGF-1-deficient mice. J Clin Invest 113(1):96-105. [PubMed: 14702113]  [MGI Ref ID J:87619]

Yu R; Yakar S; Liu YL; Lu Y; LeRoith D; Miao D; Liu JL. 2003. Liver-specific IGF-I gene deficient mice exhibit accelerated diabetes in response to streptozotocin, associated with early onset of insulin resistance. Mol Cell Endocrinol 204(1-2):31-42. [PubMed: 12850279]  [MGI Ref ID J:126277]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   17-SEP-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $195.00Female or MaleHomozygous for Igf1tm1Dlr  
Price per Pair (US dollars $)Pair Genotype
$390.00Homozygous for Igf1tm1Dlr x Homozygous for Igf1tm1Dlr  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $253.50Female or MaleHomozygous for Igf1tm1Dlr  
Price per Pair (US dollars $)Pair Genotype
$507.00Homozygous for Igf1tm1Dlr x Homozygous for Igf1tm1Dlr  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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