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| This L3mbtl1 knockout strain may be useful in further characterization of this gene and its possible role in oncogene suppression and hematopoieis. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Hanno R Hock, Massachusetts General Hospital Description
The mouse L3mbtl1 gene is the ortholog of the Drosophila tumor suppressor lethal(3)malignant brain tumor (l(3)mbt) gene. Targeting of the Drosophila gene results in embryonic lethality due to a transformation of larval brain cells.The mouse gene is one of nine mouse genes that carry MBT (malignant brain tumor)-domains (Drosophila has only 3 MBT-domain genes). The protein binds histones in a methylation state-dependent manner and contributes to a higher order chromatin structure and transcriptional repression.
Although non-redundant roles involving oncogene suppression and hematopoiesis regulation were expected with this knockout allele, none have been found. Homozygotes are born at expected frequencies, are fertile, and do not display any obvious physical or behavioral abnormalities. Complete necropsy and histological analysis of all tissues reveal no abnormalities. The gene is normally expressed in brain, but no changes in brain development, spontaneous movement or motor coordination have been observed. The processes of lymphopoiesis and hematopoiesis don't show any defects. Animals maintained for 2 years show normal survival and lifespans (even after sublethal irradiation). It is anticipated that crosses with other MBT-domain mutant lines may prove more informative in characterizing this gene's function.
Development
Exons 13-20 were flanked by loxP sites and an FRT-flanked neomycin cassette was introduced to intron 12. V6.5 (C57BL/6 x 129S4/SvJae)F1-derived embryonic stem (ES) cells were used to create the mutation. Transient expression of a Pac-cre plasmid was used to excise exons 13-20. This strain was backcrossed to C57BL/6J for two generations by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Additional Web Information
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Use of MICE by companies or for-profit entities requires a license.
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
L3mbtl1tm1.1Haho/L3mbtl1tm1.1Haho
involves: 129S4/SvJae * C57BL/6
- mortality/aging
- *normal* mortality/aging
- mice exhibit normal life span and survival after sublethal irradiation (MGI Ref ID J:166180)
- nervous system phenotype
- *normal* nervous system phenotype
- mice exhibit normal central nervous system development and function (MGI Ref ID J:166180)
- hematopoietic system phenotype
- *normal* hematopoietic system phenotype
- mice exhibit normal hematopoiesis and lymphopoiesis (MGI Ref ID J:166180)
- cellular phenotype
- *normal* cellular phenotype
- immune system phenotype
- *normal* immune system phenotype
- mice exhibit normal hematopoiesis and lymphopoiesis (MGI Ref ID J:166180)
This mouse can be used to support research in many areas including:
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | L3mbtl1tm1.1Haho | ||
|---|---|---|---|
| Allele Name | targeted mutation 1.1, Hanno Hock | ||
| Allele Type | Targeted (knock-out) | ||
| Mutation Made By | Hanno Hock, Massachusetts General Hospital | ||
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | ||
| Gene Symbol and Name | L3mbtl1, l(3)mbt-like (Drosophila) | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | H-L(3)MBT; L3MBTL; RGD1307316; ZC2HC3; dJ138B7.3; | ||
| Molecular Note | An FRT-flanked neo cassette with a 3'UTR was inserted downstream of exon 12 and an additional loxP site was inserted downstream of exon 21. Cre-mediated recombination removed exons 13 through 20. The absence of protein expression was confirmed by westernblot analysis on brain, testis, and embryonic stem cell extracts. [MGI Ref ID J:166180] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Neo Melt Curve Analysis, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Qin J; Van Buren D; Huang HS; Zhong L; Mostoslavsky R; Akbarian S; Hock H. 2010. Chromatin protein L3MBTL1 is dispensable for development and tumor suppression in mice. J Biol Chem 285(36):27767-75. [PubMed: 20592034] [MGI Ref ID J:166180]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 101043 B6129SF1/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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