Strain Name:

B6.Cg-Ccl2tm1.1Pame/J

Stock Number:

016849

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Availability:

Repository- Live

These floxed mutant mice contain loxP sites flanking exons 2-3 of the Ccl2 gene which has been modified with a red fluorescent protein gene (mcherry). Fluorescence is seen in all Ccl2-expressing cells. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3, as well as RFP, deleted in the cre-expressing tissue.

Description

Strain Information

Former Names B6.Cg-Ccl2tm2.1Pame/J    (Changed: 26-JUL-11 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   02-JUL-13
Specieslaboratory mouse
GenerationN10pN1F5 (17-DEC-17)
Generation Definitions
 
Donating Investigator Eric G Pamer,   Memorial Sloan-Kettering Cancer Center

Description
These Ccl2-RFPlox/lox mice contain loxP sites flanking exons 2-3 of the chemokine (C-C motif) ligand 2 (Ccl2 or Mcp1) gene, as well as an HA peptide followed by an aphthovirus 2A cleavage site and a cleavable red fluorescent protein (mcherry) at the 3' end of exon 3. Homozygous mice are viable and fertile. MCP1 is a chemokine required for the movement of monocytes from bone marrow into the blood stream during their recruitment to sites of injury and infection. Fluorescence is seen in all MCP1 expressing cells. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2-3, as well as RFP expression, deleted in the cre-expressing tissue. Deletion of MCP1 results in decreased monocyte emigration from the bone marrow upon LPS stimulation and increased susceptibility to infection. These mice may be useful for studying emigration of inflammatory monocytes from the bone marrow after infection.

When bred to mice carrying Tg(Tek-cre)12Flv (Stock No. 004128) and induced by LPS or bacterial infection, Cre recombinase expression in endothelial cells results in abnormal monocyte migration.

When bred to mice carrying Tg(Nes-cre)1Kln (Stock No. 003771) and induced by LPS or bacterial infection, Cre recombinase expression in the nervous system results in abnormal monocyte migration.

Development
A targeting vector was designed to insert a loxP site upstream of exon 2 and a second loxP site downstream of exon 3 of the chemokine (C-C motif) ligand 2 (Ccl2 or Mcp1) gene. The targeting vector also inserted an HA peptide followed by an aphthovirus 2A cleavage site, a cleavable red fluorescent protein (mcherry), and a frt-flanked neomycin (neo) resistance cassette at the 3' end of exon 3. The construct was electroporated into B6(Cg)-Tyrc-2J/J derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred to C57BL/6J mice. Offspring, heterozygous for this Ccl2 allele, were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J transgenic mice (Stock No. 005703) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene, resulting in a colony homozygous for the Ccl2-RFPlox/lox allele. These mice were backcrossed to C57BL/6J mice for at least 10 generations. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ccl2
004434   B6.129S4-Ccl2tm1Rol/J
014095   B6.Cg-Tg(GFAP-Ccl2)JE95Rmra/J
023347   B6N.129S1(FVB)-Ccl2tm1.2Tyos/J
View Strains carrying other alleles of Ccl2     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Chemokine, Cc Motif, Ligand 2; CCL2   (CCL2)
Human Immunodeficiency Virus Type 1, Susceptibility to   (CCL2)
Mycobacterium Tuberculosis, Susceptibility to   (CCL2)
Neural Tube Defects   (CCL2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Ccl2tm1.1Pame/Ccl2tm1.1Pame Lyz2tm1(cre)Ifo/Lyz2+

        involves: 129P2/OlaHsd * C57BL/6   (conditional)
  • immune system phenotype
  • *normal* immune system phenotype
    • LPS-induced monocyte emigration is normal   (MGI Ref ID J:171379)

Ccl2tm1.1Pame/Ccl2tm1.1Pame Tg(Alb-cre)21Mgn/0

        involves: C57BL/6 * DBA   (conditional)
  • immune system phenotype
  • *normal* immune system phenotype
    • LPS-induced monocyte emigration is normal   (MGI Ref ID J:171379)

Ccl2tm1.1Pame/Ccl2tm1.1Pame Tg(Nes-cre)1Kln/0

        involves: C57BL/6 * SJL   (conditional)
  • immune system phenotype
  • abnormal leukocyte migration
    • LPS-treated mice exhibit reduced percent of circulating Ly6Chi monocyte compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
  • increased susceptibility to bacterial infection
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow and diminished bacterial clearance compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
  • cellular phenotype
  • abnormal leukocyte migration
    • LPS-treated mice exhibit reduced percent of circulating Ly6Chi monocyte compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
  • hematopoietic system phenotype
  • abnormal leukocyte migration
    • LPS-treated mice exhibit reduced percent of circulating Ly6Chi monocyte compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)

Ccl2tm1.1Pame/Ccl2tm1.1Pame Tg(Tek-cre)12Flv/0

        involves: C3H * C57BL/6   (conditional)
  • immune system phenotype
  • abnormal leukocyte migration
    • LPS-induced monocyte emigration is decreased compared to in similarly treated wild-type mice   (MGI Ref ID J:171379)
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
  • increased susceptibility to bacterial infection
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
  • cellular phenotype
  • abnormal leukocyte migration
    • LPS-induced monocyte emigration is decreased compared to in similarly treated wild-type mice   (MGI Ref ID J:171379)
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
  • hematopoietic system phenotype
  • abnormal leukocyte migration
    • LPS-induced monocyte emigration is decreased compared to in similarly treated wild-type mice   (MGI Ref ID J:171379)
    • mice exposed to L. monocytogenes exhibit reduced Ly6Chi monocyte emigration from the bone marrow compared with similarly treated wild-type mice   (MGI Ref ID J:171379)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Inflammation

Research Tools
Cre-lox System
      loxP-flanked Sequences
      loxP-flanked Sequences: Test/Reporter
Genetics Research
      Tissue/Cell Markers: Cre-lox System

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ccl2tm1.1Pame
Allele Name targeted mutation 1.1 Eric G Pamer
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Common Name(s) Ccl2-RFPflox;
Strain of OriginB6(Cg)-Tyr
Gene Symbol and Name Ccl2, chemokine (C-C motif) ligand 2
Chromosome 11
Gene Common Name(s) AI323594; CKb10; HC11; MCAF; MCP-1; MCP-4; MCP1; NCC-1; NCC1; SCYA13; SCYL1; SMC-CF; Scya2; Sigje; expressed sequence AI323594; monocyte chemoattractant protein-1; monocyte chemotactic protein; small inducible cytokine A2; small inducible gene JE;
Molecular Note A loxP site was inserted upstream of exon 2. Exon 3 was replaced with a modified exon 3 with an HA-2A cleavable mCherry and 3' loxP site. A floxed neo cassette used for selection purposes was removed by cre-mediated recombination. [MGI Ref ID J:171379]

Genotyping

Genotyping Information

Genotyping Protocols

Ccl2tm1.1Pamealternate1,

Separated MCA


Ccl2tm1.1Pamealternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Shi C; Jia T; Mendez-Ferrer S; Hohl TM; Serbina NV; Lipuma L; Leiner I; Li MO; Frenette PS; Pamer EG. 2011. Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-like Receptor Ligands. Immunity 34(4):590-601. [PubMed: 21458307]  [MGI Ref ID J:171379]

Additional References

Ccl2tm1.1Pame related

Tagliani E; Shi C; Nancy P; Tay CS; Pamer EG; Erlebacher A. 2011. Coordinate regulation of tissue macrophage and dendritic cell population dynamics by CSF-1. J Exp Med 208(9):1901-16. [PubMed: 21825019]  [MGI Ref ID J:177594]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice may be bred together.
Mating SystemHomozygote x Homozygote         (Female x Male)   02-JUL-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Ccl2tm1.1Pame  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Ccl2tm1.1Pame x Homozygous for Ccl2tm1.1Pame  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Ccl2tm1.1Pame  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Ccl2tm1.1Pame x Homozygous for Ccl2tm1.1Pame  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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