Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129P2-Cdc14btm1.2Pzg/J    (Changed: 15-FEB-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N?pN1 Generation Definitions   Donating Investigator Dr. Pumin Zhang,   Baylor College of Medicine

Description
Cdc14b encodes a nuclear phosphatase that has been implicated in nuclear organization, centriole duplication, mitotic exit, cell cycle regulation, and DNA damage repair. Mice that are homozygous for this targeted mutation allele are viable, fertile, and normal in size. Truncated gene product (mRNA), that is not expected to produce functional protein, is detected by RT-PCR analysis of MEFs from homozygotes. Homozygotes develop cataracts and kyphosis much earlier than wildtype controls and exhibit impaired contextual fear conditioning. Homozygous females exhibit impaired fertility, with smaller litter sizes than wildtype controls. MEFs isolated from homozygotes display defective DNA damage repair and slowed growth rate in later-passages.

Development
A FRT site flanked targeting vector containing Kan/Neo selection cassette with an upstream loxP site was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 2. This construct was electroporated into 129P2/OlaHsd-derived E14Tg2a.4embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to Meox2-Cre mice to excise exon 2. The donating investigator reported that mice were backcrossed to C57BL/6 mice for 7 generations (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Use of MICE by companies or for-profit entities requires a license prior to shipping.
Use of MICE by companies or for-profit entities requires a license.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Cdc14b^tm1.2Pzg/Cdc14b^tm1.2Pzg

        B6.129P2-Cdc14b^tm1.2Pzg

• mortality/aging
• premature aging
  • including premature cataracts and kyphosis   (MGI Ref ID J:170763)
• reproductive system phenotype
• decreased litter size
  • particularly from female mice   (MGI Ref ID J:170763)
• reduced fertility
  • particularly from female mice   (MGI Ref ID J:170763)
• • reduced female fertility   (MGI Ref ID J:170763)
  • reduced male fertility   (MGI Ref ID J:170763)
• vision/eye phenotype
• abnormal cornea morphology
  • mice exhibit loose and thickened cornea compared with wild-type mice   (MGI Ref ID J:170763)
• • corneal opacity   (MGI Ref ID J:170763)
• abnormal lens morphology
  • mice exhibit invaginations in the ocular lens compared with wild-type mice   (MGI Ref ID J:170763)
• • cataracts
    • premature   (MGI Ref ID J:170763)
• cellular phenotype
• *normal* cellular phenotype
  • mouse embryonic fibroblasts exhibit normal DNA damage checkpoints   (MGI Ref ID J:170763)
• • abnormal DNA repair
    • mouse embryonic fibroblasts exhibit defective DNA damage repair compared with wild-type cells   (MGI Ref ID J:170763)
  • early cellular replicative senescence
    • in mouse embryonic fibroblasts   (MGI Ref ID J:170763)
• behavior/neurological phenotype
• *normal* behavior/neurological phenotype
  • mice exhibit normal cued conditioning   (MGI Ref ID J:170763)
• • impaired contextual conditioning behavior   (MGI Ref ID J:170763)
• skeleton phenotype
• kyphosis
  • premature   (MGI Ref ID J:170763)
• homeostasis/metabolism phenotype
• abnormal DNA repair
  • mouse embryonic fibroblasts exhibit defective DNA damage repair compared with wild-type cells   (MGI Ref ID J:170763)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Other
      DNA Repair

Cell Biology Research
DNA Damage Response

Sensorineural Research
Cataracts

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cdc14btm1.2Pzg
Allele Name		targeted mutation 1.2, Pumin Zhang
Allele Type		Targeted (knock-out)
Common Name(s)		Cdc14bdeltaE2;
Mutation Made By		Dr. Pumin Zhang,   Baylor College of Medicine
Strain of Origin		129P2/OlaHsd
Gene Symbol and Name		Cdc14b, CDC14 cell division cycle 14B
Chromosome		13
Gene Common Name(s)		2810432N10Rik; A530086E13Rik; AA472821; CDC14B3; Cdc14B1; Cdc14B2; RIKEN cDNA 2810432N10 gene; RIKEN cDNA A530086E13 gene; expressed sequence AA472821; hCDC14B;
Molecular Note		Cre-mediated recombination removed exon 2 and the neo cassette. RT-PCR confirmed the expression of two truncated transcripts that are not predicted to produce a functional protein. [MGI Ref ID J:170763]

Genotyping

Genotyping Information

Genotyping Protocols

Cdc14b^tm1.2Pzg, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wei Z; Peddibhotla S; Lin H; Fang X; Li M; Rosen JM; Zhang P. 2011. Early-onset aging and defective DNA damage response in cdc14b-deficient mice. Mol Cell Biol 31(7):1470-7. [PubMed: 21262768]  [MGI Ref ID J:170763]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. Homozygous mice have reduced fertility (particularly for homozygous females) and smaller litter sizes. If maintaining the colony by breeding homozygous mice together, a larger colony size is recommended to account for this phenotype.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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