Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129-Dpysl5tm1Gosh/J    (Changed: 23-FEB-12 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N7pN1 Generation Definitions   Donating Investigator Pappachan Kolattukudy,   University of Central Florida

Description
In this strain an IRES-lacZ-neo cassette replaces exons 2-6 of the dihydropyrimidinase-like 5 (Dpysl4 or Crmp5) gene. Homozygotes are viable and fertile. CRMP-5 is a member of a family of phosphoproteins that are involved in dendritic length and branching, semaphorin signaling pathway, growth cone collapse, and neurite extension after binding with tubulin heterodimers. It is expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons. These mice exhibit abnormally small Purkinje cells in the cerebellum during the period of dendritic branching. They also display long-term depression of excitatory synaptic transmissions. This strain may be useful for studying nervous system developmental disorders.

Development
A targeting vector was designed to replace exons 2-6 of the dihydropyrimidinase-like 5 (Dpysl4 or Crmp5) gene with an internal ribosome entry site (IRES)-β-galactosidase (lacZ) followed by a neomycin resistance cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric males were bred with C57BL/6 females for germline transmission. A chimeric male offspring was then bred to 129/SvE females. Upon arrival at The Jackson Laboratory, mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation.

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Dpysl5^tm1Gosh/Dpysl5^tm1Gosh

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• nervous system phenotype
• abnormal Purkinje cell morphology
  • at P21 and P28, Purkinje cell body is small and the diameter of primary dendrites is thinker than in wild-type mice   (MGI Ref ID J:168982)
  • however, Purkinje cells are normal at P14   (MGI Ref ID J:168982)
• • abnormal Purkinje cell dendrite morphology
    • at P21 and P28, Purkinje cell body is small and the diameter of primary dendrites is thinker than in wild-type mice   (MGI Ref ID J:168982)
• abnormal neuron physiology
  • following BDNF treatment, primary cultured neurons fail to exhibit an increase in primary dendrites unlike similarly treated wild-type neurons   (MGI Ref ID J:168982)
• absent long term depression
  • following conjunctive stimulation   (MGI Ref ID J:168982)
• behavior/neurological phenotype
• limb grasping
  • in adult mice   (MGI Ref ID J:168982)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
lacZ expression in neural tissue
Cerebellar Defects
      Purkinje cell defect

Research Tools
Genetics Research
      Tissue/Cell Markers: neurons
Neurobiology Research
      cell marker

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Dpysl5tm1Gosh
Allele Name		targeted mutation 1, Yoshio Goshima
Allele Type		Targeted (Reporter)
Mutation Made By		Yoshio Goshima,   Yokohama City University Graduate School
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Site of Expression		Dpysl5 is normally expressed throughout the nervous system after P14 and is specifically involved in filopodia and growth cone morphology in neurons.
Gene Symbol and Name		Dpysl5, dihydropyrimidinase-like 5
Chromosome		5
Gene Common Name(s)		CRAM; CRMP-5; CRMP5; Ulip6;
Molecular Note		Exons 2 through 6 were replaced with an IRES/lacZ/neo cassette. The absence of protein expression was confirmed by western blot analysis on brain extracts. [MGI Ref ID J:168982]

Genotyping

Genotyping Information

Genotyping Protocols

Dpysl5^tm1Gosh,

Separated MCA

Dpysl5^tm1Gosh, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yamashita N; Mosinger B; Roy A; Miyazaki M; Ugajin K; Nakamura F; Sasaki Y; Yamaguchi K; Kolattukudy P; Goshima Y. 2011. CRMP5 (Collapsin Response Mediator Protein 5) Regulates Dendritic Development and Synaptic Plasticity in the Cerebellar Purkinje Cells. J Neurosci 31(5):1773-9. [PubMed: 21289187]  [MGI Ref ID J:168982]

Additional References

Dpysl5^tm1Gosh related

Veyrac A; Reibel S; Sacquet J; Mutin M; Camdessanche JP; Kolattukudy P; Honnorat J; Jourdan F. 2011. CRMP5 regulates generation and survival of newborn neurons in olfactory and hippocampal neurogenic areas of the adult mouse brain. PLoS One 6(10):e23721. [PubMed: 21991301]  [MGI Ref ID J:179635]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, homozygous mice may be bred together.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
	002448 129S1/SvImJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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