Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N12pN1 Generation Definitions   Donating Investigator Albert Bendelac ,   University of Chicago

Description
Sequence encoding the cytoplasmic tail that governs steady-state accumulation of CD1D1 (CD1d1 antigen) protein in endosome compartments was deleted in these targeted mutation mice. A neomycin gene was left in the 3' untranslated region of Cd1d1. Homozygotes show multiple and selective abnormalities in intracellular trafficking of CD1D and lipid antigen presentation, as well as natural killer T (NKT) cell development. On average, the mutant CD1-TD protein is expressed on the cell surface at 40% higher density than with wildtype protein in thymocytes, B cells and dendritic cells. Confocal microscopic analysis shows that the abundant intracellular stores of CD1D normally found in lysosome-associated membrane protein 1-positive (LAMP-1⁺) late endosomes and lysosomes is markedly depleted. A marked reduction in the number of Vα14 natural killer T (NKT) cells in the thymus and peripheral tissues including the spleen and liver was also noted. These mice may be helpful in studies of CD1D trafficking and antigen presentation pathways as well as the development of CD1D-restricted T cells.

Development
A vector incorporating a deletion of the nucleotides encoding the SAYQDIR carboxy-terminus (cytoplasmic tail) segment of the targeted gene followed by a floxed neomycin resistance cassette was introduced to 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. The donating investigator reported that this strain was backcrossed to C57BL/6 (Taconic) for 12 generations by the donating laboratory (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository (after breeding once to C57BL/6NJ). While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the donating investigator backcrossed the mice to the C57BL/6J inbred mice available from Taconic prior to sending to The Jackson Laboratory Repository.

Control Information

	Control
	000664 C57BL/6J	(approximate)
	005304 C57BL/6NJ	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cd1d1

017294	B6(C)-Cd1d1tm1.2Aben/J
019418	B6(Cg)-Tg(Lck-Cd1d1)1Aben/J
008881	B6.129S6-Cd1d1/Cd1d2tm1Spb/J
016929	C57BL/6-Cd1d1tm1.1Aben/J
006330	NOD.129S6(B6)-Cd1d1/Cd1d2tm1Spb/SbwJ

View Strains carrying other alleles of Cd1d1     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Cd1d1^tm2Aben/Cd1d1^tm2Aben

        B6.129P2-Cd1d1^tm2Aben

• immune system phenotype
• abnormal antigen presentation
  • thymocytes and peritoneal macrophages exhibit impaired antigen presentation of alphaGalCer compared with wild-type cells   (MGI Ref ID J:174337)
• decreased NK T cell number
  • mice exhibit a reduction of Valpha14 NKT cells in the thymus and peripheral tissues such as spleen and liver due to failure of positive selection compared with wild-type mice   (MGI Ref ID J:174337)
• hematopoietic system phenotype
• decreased NK T cell number
  • mice exhibit a reduction of Valpha14 NKT cells in the thymus and peripheral tissues such as spleen and liver due to failure of positive selection compared with wild-type mice   (MGI Ref ID J:174337)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd1d1^tm2Aben/Cd1d1^tm2Aben

        involves: 129P2/OlaHsd * C57BL/6

• immune system phenotype
• abnormal antigen presentation
  • thymocytes and peritoneal macrophages exhibit impaired antigen presentation of alphaGalCer compared with wild-type cells   (MGI Ref ID J:174337)
• decreased NK T cell number
  • mice exhibit a reduction of Valpha14 NKT cells in the thymus and peripheral tissues such as spleen and liver due to failure of positive selection compared with wild-type mice   (MGI Ref ID J:174337)
• hematopoietic system phenotype
• decreased NK T cell number
  • mice exhibit a reduction of Valpha14 NKT cells in the thymus and peripheral tissues such as spleen and liver due to failure of positive selection compared with wild-type mice   (MGI Ref ID J:174337)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
      specific T cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cd1d1tm2Aben
Allele Name		targeted mutation 2, Albert Bendelac
Allele Type		Targeted (other)
Common Name(s)		CDI-TD;
Mutation Made By		Albert Bendelac ,   University of Chicago
Strain of Origin		129P2/OlaHsd
Gene Symbol and Name		Cd1d1, CD1d1 antigen
Chromosome		3
Gene Common Name(s)		AI747460; CD1.1; CD1A; CD1a antigen; CD1d antigen; Cd1; Cd1a; Cd1d; Ly-38; R3; expressed sequence AI747460; lymphocyte antigen 38;
Molecular Note		The nucleotides encoding the SAYQDIR carboxy-terminus (cytoplasmic tail) segment were deleted. A floxed neo cassette was inserted downstream of the last exon. [MGI Ref ID J:174337]

Genotyping

Genotyping Information

Genotyping Protocols

Cd1d1^tm2Aben, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Chiu YH; Park SH; Benlagha K; Forestier C; Jayawardena-Wolf J; Savage PB; Teyton L; Bendelac A. 2002. Multiple defects in antigen presentation and T cell development by mice expressing cytoplasmic tail-truncated CD1d. Nat Immunol 3(1):55-60. [PubMed: 11731798]  [MGI Ref ID J:174337]

Additional References

Cd1d1^tm2Aben related

Gordy LE; Bezbradica JS; Flyak AI; Spencer CT; Dunkle A; Sun J; Stanic AK; Boothby MR; He YW; Zhao Z; Van Kaer L; Joyce S. 2011. IL-15 regulates homeostasis and terminal maturation of NKT cells. J Immunol 187(12):6335-45. [PubMed: 22084435]  [MGI Ref ID J:180390]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintained as a live colony, homozygotes or heterozygotes may be bred.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J	(approximate)
	005304 C57BL/6NJ	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering Information
JAX^® Mice
Surgical and Preconditioning Services
JAX^® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.