Strain Name:

B6.129S(FVB)-Tsxtm1.2Jtl/J

Stock Number:

016955

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Cryopreserved - Ready for recovery

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This strain carries a knock-out mutation of the Tsx gene. Among the phenotypic traits displayed in males are enhanced short-term memory and a reduced fear response.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S-Tsxtm1.2Jtl/J    (Changed: 28-OCT-11 )
B6.129S-Tsxtm1.1Jtl/J    (Changed: 28-OCT-11 )
B6J.129S-Tsxtm1Jtl/J    (Changed: 25-JUL-11 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?+F3
Generation Definitions
 
Donating Investigator Jeannie Lee,   Massachusetts General Hospital, HHMI

Description
Tsx (testis specific X-linked gene), situated in the X-inactivation center of mouse Chromosome X, encodes a non-translated RNA (a noncoding RNA). These mice carry a targeted knockout of the gene. Hemizygous mutant males (-/Y) have smaller testes due to germ cell apoptosis, but are fertile. Homozygous females may have mildly reduced fertility. Expression is eliminated from all hemizygous male tissues tested, including the testes and lung, as determined by Northern blot. Male mice lacking this noncoding RNA display less fear and enhanced short-term memory. X chromosome inactivation (XCI) is mildly affected in embryonic stem cells which are severely growth-retarded, differentiate poorly, and show increased cell death.

Development
A 2.1 kb region encompassing the predicted promoter region upstream of exon 1 and 160 bp of intron 1 was flanked by loxP sites and preceded by an FRT-flanked PGK-neomycin selection cassette. 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells were used to create the mutation. Resultant progeny were crossed with 129S4 background FLP-expressing mice (see Stock No. 003946) to excise the neomycin cassette, then crossed with C57BL/6 background cre-expressing mice (see Stock No. 003724) to excise the floxed exon. The donating investigator reported the strain was backcrossed to C57BL/6J for seven generations (see SNP results below) prior to sending to The Jackson Laboratory Repository. The mice were bred to C57BL/6J mice (Stock No. 000664) for at least one generation to generate the live colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the F3-F4 generation living colony at The Jackson Laboratory Repository. While the 26 markers throughout the somatic genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N or mixed C57BL/6N;C57BL/6J genetic background.

Control Information

  Control
   000664 C57BL/6J (approximate)
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tsxtm1.2Jtl/Tsxtm1.2Jtl

        B6.129S(FVB)-Tsxtm1.2Jtl
  • reproductive system phenotype
  • decreased litter size
    • homozygous females mated to hemizygous males or wild-type males produce smaller litters and litter ratios indicate a slight increase in female births   (MGI Ref ID J:177104)
    • litters produced using hemizygous males crossed to wild-type females are similar to controls   (MGI Ref ID J:177104)
  • reduced female fertility   (MGI Ref ID J:177104)

Tsxtm1.2Jtl/Y

        B6.129S(FVB)-Tsxtm1.2Jtl
  • behavior/neurological phenotype
  • enhanced contextual conditioning behavior
    • short-term contextual conditioning behavior (1 hour retention interval) as measured by the fear conditioning test is improved as compared to wild-type   (MGI Ref ID J:177104)
    • higher levels of freezing in the fear conditioning test are observed only in males   (MGI Ref ID J:177104)
  • endocrine/exocrine gland phenotype
  • small testis   (MGI Ref ID J:177104)
    • testis hypoplasia
      • germ cell apoptosis is significantly increased at 7 days, 14 days and 2 months, however, by 1 year apoptosis levels do not differ from wild-type   (MGI Ref ID J:177104)
      • apoptosis is greatest at 14 days (5X higher than controls) during meiotic prophase I   (MGI Ref ID J:177104)
      • TUNEL staining indicates that a subset of pachytene-stage spermatocytes inappropriately undergo apoptosis   (MGI Ref ID J:177104)
  • reproductive system phenotype
  • small testis   (MGI Ref ID J:177104)
    • testis hypoplasia
      • germ cell apoptosis is significantly increased at 7 days, 14 days and 2 months, however, by 1 year apoptosis levels do not differ from wild-type   (MGI Ref ID J:177104)
      • apoptosis is greatest at 14 days (5X higher than controls) during meiotic prophase I   (MGI Ref ID J:177104)
      • TUNEL staining indicates that a subset of pachytene-stage spermatocytes inappropriately undergo apoptosis   (MGI Ref ID J:177104)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research

Cell Biology Research

Neurobiology Research
Behavioral and Learning Defects

Reproductive Biology Research
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tsxtm1.2Jtl
Allele Name targeted mutation 1.2, Jeannie T Lee
Allele Type Targeted (Null/Knockout)
Common Name(s) TsxKO;
Mutation Made By Jeannie Lee,   Massachusetts General Hospital, HHMI
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Tsx, testis specific X-linked gene
Chromosome X
Gene Common Name(s) AV207184; expressed sequence AV207184;
Molecular Note The construct includes an FRT-flanked PGK-neomycin selection cassette upstream of exon 1 followed by a 2.1 kb region encompassing the predicted promoter region upstream of exon 1 and 160 bp of intron 1 flanked by loxP sites. The selection cassette was subsequently excised by FLP recombinase. Cre mediated recombination removed exon 1. Northern blot analysis confirmed loss of all RNA isoforms. [MGI Ref ID J:177104]

Genotyping

Genotyping Information

Genotyping Protocols

Tsxtm1.1Jtl, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Anguera MC; Ma W; Clift D; Namekawa S; Kelleher RJ 3rd; Lee JT. 2011. Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain. PLoS Genet 7(9):e1002248. [PubMed: 21912526]  [MGI Ref ID J:177104]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHeterozygous or homozygous females and hemizygous (-/Y) males may be bred.

Heterozygous female x hemizygous male crosses and crosses to inbred C57BL/6J mice are reported to produce progeny at predicted Mendelian ratios with litter sizes of approximately 7 pups. When homozygous females are crossed with hemizygous males, a mild but significant sex ratio distortion favoring females is seen among progeny in conjunction with smaller than average litter sizes. This suggests that homozygous females have mildly reduced fertility.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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