Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+F3 Generation Definitions   Donating Investigator Jeannie Lee,   Massachusetts General Hospital, HHMI

Description
Tsx (testis specific X-linked gene), situated in the X-inactivation center of mouse Chromosome X, encodes a non-translated RNA (a noncoding RNA). These mice carry a targeted knockout of the gene. Hemizygous mutant males (-/Y) have smaller testes due to germ cell apoptosis, but are fertile. Homozygous females may have mildly reduced fertility. Expression is eliminated from all hemizygous male tissues tested, including the testes and lung, as determined by Northern blot. Male mice lacking this noncoding RNA display less fear and enhanced short-term memory. X chromosome inactivation (XCI) is mildly affected in embryonic stem cells which are severely growth-retarded, differentiate poorly, and show increased cell death.

Development
A 2.1 kb region encompassing the predicted promoter region upstream of exon 1 and 160 bp of intron 1 was flanked by loxP sites and preceded by an FRT-flanked PGK-neomycin selection cassette. 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells were used to create the mutation. Resultant progeny were crossed with 129S4 background FLP-expressing mice (see Stock No. 003946) to excise the neomycin cassette, then crossed with C57BL/6 background cre-expressing mice (see Stock No. 003724) to excise the floxed exon. This strain was backcrossed to C57BL/6J for 7 generations by the donating laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tsx^tm1.2Jtl/Tsx^tm1.2Jtl

        B6.129S(FVB)-Tsx^tm1.2Jtl

• reproductive system phenotype
• decreased litter size
  • homozygous females mated to hemizygous males or wild-type males produce smaller litters and litter ratios indicate a slight increase in female births   (MGI Ref ID J:177104)
  • litters produced using hemizygous males crossed to wild-type females are similar to controls   (MGI Ref ID J:177104)
• reduced female fertility   (MGI Ref ID J:177104)

Tsx^tm1.2Jtl/Y

        B6.129S(FVB)-Tsx^tm1.2Jtl

• behavior/neurological phenotype
• enhanced contextual conditioning behavior
  • short-term contextual conditioning behavior (1 hour retention interval) as measured by the fear conditioning test is improved as compared to wild-type   (MGI Ref ID J:177104)
  • higher levels of freezing in the fear conditioning test are observed only in males   (MGI Ref ID J:177104)
• endocrine/exocrine gland phenotype
• small testis   (MGI Ref ID J:177104)
• • testis hypoplasia
    • germ cell apoptosis is significantly increased at 7 days, 14 days and 2 months, however, by 1 year apoptosis levels do not differ from wild-type   (MGI Ref ID J:177104)
    • apoptosis is greatest at 14 days (5X higher than controls) during meiotic prophase I   (MGI Ref ID J:177104)
    • TUNEL staining indicates that a subset of pachytene-stage spermatocytes inappropriately undergo apoptosis   (MGI Ref ID J:177104)
• reproductive system phenotype
• small testis   (MGI Ref ID J:177104)
• • testis hypoplasia
    • germ cell apoptosis is significantly increased at 7 days, 14 days and 2 months, however, by 1 year apoptosis levels do not differ from wild-type   (MGI Ref ID J:177104)
    • apoptosis is greatest at 14 days (5X higher than controls) during meiotic prophase I   (MGI Ref ID J:177104)
    • TUNEL staining indicates that a subset of pachytene-stage spermatocytes inappropriately undergo apoptosis   (MGI Ref ID J:177104)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research

Cell Biology Research

Neurobiology Research
Behavioral and Learning Defects

Reproductive Biology Research
Fertility Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tsxtm1.2Jtl
Allele Name		targeted mutation 1.2, Jeannie T Lee
Allele Type		Targeted (knock-out)
Common Name(s)		TsxKO;
Mutation Made By		Jeannie Lee,   Massachusetts General Hospital, HHMI
Strain of Origin		129S6/SvEvTac
Gene Symbol and Name		Tsx, testis specific X-linked gene
Chromosome		X
Gene Common Name(s)		AV207184; expressed sequence AV207184;
Molecular Note		The construct includes an FRT-flanked PGK-neomycin selection cassette upstream of exon 1 followed by a 2.1 kb region encompassing the predicted promoter region upstream of exon 1 and 160 bp of intron 1 flanked by loxP sites. The selection cassette was subsequently excised by FLP recombinase. Cre mediated recombination removed exon 1. Northern blot analysis confirmed loss of all RNA isoforms. [MGI Ref ID J:177104]

Genotyping

Genotyping Information

Genotyping Protocols

Tsx^tm1.1Jtl, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Anguera MC; Ma W; Clift D; Namekawa S; Kelleher RJ 3rd; Lee JT. 2011. Tsx produces a long noncoding RNA and has general functions in the germline, stem cells, and brain. PLoS Genet 7(9):e1002248. [PubMed: 21912526]  [MGI Ref ID J:177104]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry

Heterozygous or homozygous females and hemizygous (-/Y) males may be bred. Heterozygous female x hemizygous male crosses and crosses to inbred C57BL/6J mice are reported to produce progeny at predicted Mendelian ratios with litter sizes of approximately 7 pups. When homozygous females are crossed with hemizygous males, a mild but significant sex ratio distortion favoring females is seen among progeny in conjunction with smaller than average litter sizes. This suggests that homozygous females have mildly reduced fertility.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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