Strain Name:

B6.129(Cg)-Foxp3tm3(DTR/GFP)Ayr/J

Stock Number:

016958

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
Foxp3DTR mutant mice express knocked-in human diphtheria toxin receptor and EGFP genes from the Foxp3 locus--without disrupting expression of the endogenous Foxp3 gene. These mice may be useful for regulatory T cell visualizing and ablation.

Description

Strain Information

Former Names B6.129(Cg)-Foxp3tm3Ayr/J    (Changed: 02-APR-14 )
B6N.129(Cg)-Foxp3tm3Ayr/J    (Changed: 03-SEP-13 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Hemizygote         (Female x Male)   31-DEC-13
Specieslaboratory mouse
GenerationN8+pN1f3 (11-DEC-13)
Generation Definitions
 
Donating Investigator Alexander Rudensky,   University of Washington

Description
Foxp3DTR mutant mice contain an internal ribosome entry site (IRES), a human diphtheria toxin receptor (DTR), and an enhanced green fluorescent protein (EGFP) downstream of the internal stop codon of the forkhead box P3 (Foxp3) gene. FOXP3 is a transcription factor required for the development and function of regulatory T (T reg) cells, which are required for suppression of self-reactive T cells and prevention of some autoimmune diseases. In these mice, DTR-EGFP expression is evident in FOXP3+ Treg cells. Diphtheria toxin (DT) administration results in ablation of Treg cells in thymus, lymph nodes, and spleen 2 days after injection, with cell numbers rebounding 10-15 days post-injection. Neonates injected with DT daily all die from lymphoproliferative disease, indicated by failure to thrive, lack of mobility, ventral skin lesions, hunched posture, and conjunctivitis, within 27 days of birth. With the same DT treatment, adults exhibit a more rapid development of autoimmune disease and die within 3 weeks. These mice show an increase in CD4+ T cell activation, as well as an increase in the number of B cells, macrophages, granulocytes, natural killer, and dendritic cells in the spleen and lymph nodes. Homozygous females and hemizygous males are viable, fertile, and normal in size. These mice may be useful for visualizing and specifically eliminating regulatory T cells, and for studying autoimmunity and immune dysfunction.

Development
The Foxp3DTR targeting vector was designed with an internal ribosome entry site (IRES), a human diphtheria toxin receptor (DTR), and an enhanced green fluorescent protein (EGFP), followed by a frt-flanked neomycin (neo) resistance cassette inserted downstream of the internal stop codon of the X-linked forkhead box P3 (Foxp3) gene. This construct was injected into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The donating investigator reported that the resulting mice were backcrossed to C57BL/6NTac mice for at least 8 generations (see SNP note below). Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6NJ inbred mice (Stock No. 005304) for at least one generation.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6 genetic background.

Control Information

  Control
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls

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009118   B6.129P2(Cg)-Hprttm18(Ple90-EGFP)Ems/Mmjax
009353   B6.129P2(Cg)-Hprttm20(Ple53-EGFP)Ems/Mmjax
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010770   B6.129P2(Cg)-Hprttm34(Ple186-EGFP)Ems/Mmjax
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016934   B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
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012644   B6.129S7-Pcdhgtm2Xzw/J
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008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
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022149   B6.Cg-Tg(CSNK1D,-EGFP)432Yfu/J
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006649   B6;129P2-Olfr17tm5(Olfr6)Mom/MomJ
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010827   B6;C3-Tg(FOXJ1-EGFP)85Leo/J
010930   B6;CB-Tg(Pbsn-Hpn,-GFP)DVv/J
010704   B6;CBA-Tg(ATP6V1B1-EGFP)1Rnel/Mmjax
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
021588   B6;CBA-Tg(Gast-EGFP)1Tcw/J
007986   B6;CBA-Tg(H*/Olfr16-GFP)11Mom/MomJ
007987   B6;CBA-Tg(H*/Olfr16-GFP)25Mom/MomJ
007979   B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ
007980   B6;CBA-Tg(H/Olfr16-GFP)4Mom/MomJ
007981   B6;CBA-Tg(H/Olfr16-GFP)6Mom/MomJ
007984   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)11Mom/MomJ
007985   B6;CBA-Tg(H/Olfr16-taumCherry,-tauGFP)13Mom/MomJ
007982   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)8Mom/MomJ
007983   B6;CBA-Tg(H/Olfr16-taumRFP,-tauGFP)9Mom/MomJ
007978   B6;CBA-Tg(Hf/Olfr16-GFP)47Mom/MomJ
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011070   B6;CBA-Tg(Thy1-EGFP)SJrs/NdivJ
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015814   B6;CBA-Tg(Thy1-spH)64Vnmu/FrkJ
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018896   C57BL/6J-Tg(Krt6,-cre,-Cerulean)2Grsr/Grsr
018898   C57BL/6J-Tg(Krt6,-cre,-Cerulean)4Grsr/Grsr
018899   C57BL/6J-Tg(Krt6,-cre,-Cerulean)5Grsr/Grsr
009593   C57BL/6J-Tg(Pomc-EGFP)1Low/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
019363   C57BL/6J-Tg(Trp63,-cre,-Cerulean)10Grsr/Grsr
018792   C57BL/6J-Tg(Trp63,-cre,-Cerulean)4Grsr/GrsrJ
024898   C57BL/6J-Tg(tetO-EGFP/Rpl10a)5aReij/J
018151   C57BL/6N-Krt17tm1(cre,Cerulean)Murr/GrsrJ
024753   C57BL/6N-Tg(Ddx25*-EGFP)1Mld/J
024752   C57BL/6N-Tg(Ddx25-EGFP)1Mld/J
008234   CB6-Tg(CAG-EGFP/CETN2)3-4Jgg/J
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007898   CBy.Cg-Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
007075   CByJ.B6-Tg(CAG-EGFP)1Osb/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
010548   D1.FVB(Cg)-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008450   FVB-Tg(CAG-luc,-GFP)L2G85Chco/J
003718   FVB-Tg(GadGFP)45704Swn/J
010947   FVB-Tg(Gstm5-EGFP)1Ilis/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
017484   FVB-Tg(JPH3-GFP,-JPH3*)GXwy/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005688   FVB-Tg(Rag2-EGFP)1Mnz/J
024636   FVB.B6-Tg(CAG-cat,-EGFP)1Rbns/KrnzJ
012429   FVB.Cg-Gt(ROSA)26Sortm1(CAG-lacZ,-EGFP)Glh/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
022735   FVB.Cg-Tg(Cspg4-EGFP*)HDbe/J
016573   FVB.Cg-Tg(SMN2)89Ahmb Smn1tm1Msd Tg(S100B-EGFP)1Wjt Tg(SMN2*delta7)4299Ahmb/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
008200   FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J
018393   FVB/N-Tg(CAG-EGFP,TGFB1*)C8Kul/J
009354   FVB/N-Tg(Dazl-EGFP)10Rarp/J
025062   FVB/N-Tg(Figla-EGFP,-icre)ZP3Dean/Mmjax
018548   FVB/N-Tg(GFAP-Cadm1/EGFP)42Oje/J
003257   FVB/N-Tg(GFAPGFP)14Mes/J
025097   NOD.129X1(Cg)-Foxp3tm2Tch/DvsJ
013116   NOD.B6-Tg(Ins2-luc/EGFP/TK)300Kauf/J
013233   NOD.B6-Tg(Itgax-cre,-EGFP)4097Ach/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
021937   NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CAG-EGFP)1Osb/SzJ
017619   NOD.Cg-Prkdcscid Tg(CAG-EGFP)1Osb/KupwJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
010542   NOD.FVB-Tg(CAG-luc,-GFP)L2G85Chco/FathJ
008547   NOD.FVB-Tg(ITGAM-DTR/EGFP)34Lan/JdkJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1cJbs/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
012881   STOCK Ascl1tm1Reed/J
008666   STOCK Fmn1tm1Made/J
016961   STOCK Foxp3tm9(EGFP/cre/ERT2)Ayr/J
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
017596   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#aAhmb/J
017597   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(SMN2)89Ahmb Smn1tm1Msd Tg(SMN2*delta7)4299Ahmb Tg(tetO-SMN2,-luc)#bAhmb/J
025671   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Tg(tetO-Fgf10)1Jaw/SpdlJ
024746   STOCK Gt(ROSA)26Sortm1.1(rtTA,EGFP)Nagy Hprttm1(tetO-Dkk1)Spdl Tg(TCF/Lef1-lacZ)34Efu/J
018903   STOCK Gt(ROSA)26Sortm2(EGFP/cre)Alj/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
008876   STOCK Hprttm11(Ple176-EGFP/cre)Ems/Mmjax
009349   STOCK Hprttm31(Ple67-EGFP)Ems/Mmjax
009594   STOCK Hprttm32(Ple112-EGFP)Ems/Mmjax
017530   STOCK Igs2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J
013749   STOCK Iis2tm1(ACTB-EGFP,-tdTomato)Luo/J
013751   STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo/J
017932   STOCK Iis3tm1.1(ACTB-EGFP*)Luo/J
017923   STOCK Iis3tm2.1(ACTB-EGFP*,-tdTomato)Luo/J
017701   STOCK Kiss1tm1.1(cre/EGFP)Stei/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
005692   STOCK Nphs1tm1Rkl/J
006702   STOCK Ntstm1Mom/MomJ
006622   STOCK Olfr151tm10Mom/MomJ
006646   STOCK Olfr151tm11(Olfr160)Mom/MomJ
006692   STOCK Olfr151tm16(Olfr160/Olfr161)Mom/MomJ
006627   STOCK Olfr151tm4Mom/MomJ
006626   STOCK Olfr151tm6Mom/MomJ
006625   STOCK Olfr151tm7Mom/MomJ
006624   STOCK Olfr151tm8Mom/MomJ
006623   STOCK Olfr151tm9Mom/MomJ
006740   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)AMom/MomJ
006741   STOCK Olfr160tm1(Olfr151)Mom Tg(Olfr151,taulacZ)BMom/MomJ
006647   STOCK Olfr160tm1(Olfr151)Mom/MomJ
006636   STOCK Olfr160tm5(Cnga2)Mom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006650   STOCK Olfr17tm6(Olfr713)Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
009061   STOCK Osr1tm1(EGFP/cre/ERT2)Amc/J
022757   STOCK Prg4tm1(GFP/cre/ERT2)Abl/J
006770   STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
006633   STOCK Vmn1r49tm3Mom/MomJ
010911   STOCK Wt1tm1(EGFP/cre)Wtp/J
017472   STOCK Tg(Acp5-CFP,Ibsp-YFP,Dmp1-RFP)1Pmay/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
017919   STOCK Tg(CAG-EGFP,-dsRed2/RNAi:Tardbp)6Zxu/J
011106   STOCK Tg(CAG-GFP*)1Hadj/J
013753   STOCK Tg(CAG-KikGR)33Hadj/J
013754   STOCK Tg(CAG-KikGR)75Hadj/J
019082   STOCK Tg(CMV-GFP,-BBS4)4T25Vcs/J
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
017468   STOCK Tg(Col1a1*3.6-Cyan)2Rowe/J
018322   STOCK Tg(Cp-EGFP)25Gaia/ReyaJ
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
017952   STOCK Tg(Isl1-EGFP*)1Slp/J
023965   STOCK Tg(Krt17-EGFP)#Cou/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
008579   STOCK Tg(PSCA-EGFP)1Witt/J
024578   STOCK Tg(Pax6-GFP/cre)1Rilm/J
023345   STOCK Tg(Pgk1-Ccnb1/EGFP)1Aklo/J
012276   STOCK Tg(Piwil2/EGFP)1Ghan/J
012277   STOCK Tg(Piwil4/EGFP)1Ghan/J
012452   STOCK Tg(Rr5-GFP/cre)1Sapc/J
006570   STOCK Tg(SMN2)89Ahmb Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
018148   STOCK Tg(Slc17a8-EGFP)1Edw/SealJ
013752   STOCK Tg(TCF/Lef1-HIST1H2BB/EGFP)61Hadj/J
003658   STOCK Tg(TIE2GFP)287Sato/J
021226   STOCK Tg(Thy1-Brainbow3.1)18Jrs/J
021225   STOCK Tg(Thy1-Brainbow3.1)3Jrs/J
021227   STOCK Tg(Thy1-Brainbow3.2)7Jrs/J
007788   STOCK Tg(Thy1-EGFP)MJrs/J
016981   STOCK Tg(Uchl1-HIST2H2BE/mCherry/EGFP*)FSout/J
025193   STOCK Tg(Vmn1r232-Mapt/EGFP)1Dlc/J
018281   STOCK Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax
006129   STOCK Tg(Zp3-EGFP)1Dean/J
023724   STOCK Tg(mI56i-cre,EGFP)1Kc/J
017755   STOCK Tg(tetO-GCAMP2)12iRyu/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
017918   STOCK Tg(tetO-MAML1*/EGFP)2Akar/J
017906   STOCK Tg(tetO-hop/EGFP,-COP4/mCherry)6Kftnk/J
012345   STOCK Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo/J
View Strains carrying other alleles of GFP     (362 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Diabetes Mellitus, Insulin-Dependent; IDDM   (FOXP3)
Immunodysregulation, Polyendocrinopathy, and Enteropathy, X-Linked;   (FOXP3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Foxp3tm3(DTR/GFP)Ayr/Foxp3tm3(DTR/GFP)Ayr

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • mortality/aging
  • increased sensitivity to induced morbidity/mortality
    • with injection of diptheria toxin (DT) in newborns at 12 hours after birth and then every other day thereafter, 40% of mutants become moribund by 24 days   (MGI Ref ID J:117743)
    • remaining treated female mutants at weaning are moribund within 27 days of age   (MGI Ref ID J:117743)
    • adult female mutants treated with DT become moribund beginning at 10 days post-injection and all are dead after 3 weeks of treatment (regulatory T cell ablation)   (MGI Ref ID J:117743)
  • growth/size/body phenotype
  • weight loss
    • observed in newborn and adult mutants treated with DT   (MGI Ref ID J:117743)
    • cachexia
      • occurs in newborn and adult mutants upon DT treatment   (MGI Ref ID J:117743)
  • immune system phenotype
  • abnormal CD4-positive, alpha-beta T cell physiology
    • Foxp3-null CD4+ T cells from DT-treated female mutants have higher T cell activation marker levels than cells from treated controls, as well as proliferation markers   (MGI Ref ID J:117743)
  • abnormal dendritic cell physiology
    • in mutants after Treg ablation   (MGI Ref ID J:117743)
  • conjunctivitis
    • newborns treated with DT 12 hours after birth exhibit conjunctivitis; adults treated with DT show severe conjunctivitis   (MGI Ref ID J:117743)
  • decreased T cell number
    • injection of 50 ug/kg DT at 0 and 24 hours almost completely eliminates all regulatory T cells (Treg) in 2 days   (MGI Ref ID J:117743)
    • thymic T cells rebound to 47% of pre-treatment numbers after 4 days and recovers fully by 10 days; peripheral (lymph nodes and spleen) (Treg) numbers recover to 8 and 6% by 2 days and fully recover to pre-treatment levels between 10 and 15 days after injection   (MGI Ref ID J:117743)
  • dermatitis
    • massive lymphocytic and mononuclear infiltrates in epidermis seen at 3 weeks with DT treatment   (MGI Ref ID J:117743)
  • enlarged spleen
    • observed in 3-week old mutants treated daily with DT   (MGI Ref ID J:117743)
    • also seen in 3-month old adult mutants treated with DT   (MGI Ref ID J:117743)
  • increased dendritic cell number
    • numbers are elevated after Treg ablation   (MGI Ref ID J:117743)
  • increased susceptibility to autoimmune disorder
    • female mutants treated with DT rapidly develop terminal autoimmune disease resulting from (Treg) elimination; adult mice develop disease more rapidly than newborn mutants   (MGI Ref ID J:117743)
  • liver inflammation
    • massive lymphocytic and mononuclear infiltrates in liver sinusoids seen at 3 weeks with DT treatment   (MGI Ref ID J:117743)
  • lung inflammation
    • massive lymphocytic and mononuclear infiltrates in lung interstitium seen at 3 weeks with DT treatment   (MGI Ref ID J:117743)
  • lymph node inflammation
    • observed in 3-week old mutants treated daily with DT compared to untreated mutant littermates   (MGI Ref ID J:117743)
    • also seen in 3-month old adult mutants treated with DT   (MGI Ref ID J:117743)
  • hematopoietic system phenotype
  • abnormal CD4-positive, alpha-beta T cell physiology
    • Foxp3-null CD4+ T cells from DT-treated female mutants have higher T cell activation marker levels than cells from treated controls, as well as proliferation markers   (MGI Ref ID J:117743)
  • decreased T cell number
    • injection of 50 ug/kg DT at 0 and 24 hours almost completely eliminates all regulatory T cells (Treg) in 2 days   (MGI Ref ID J:117743)
    • thymic T cells rebound to 47% of pre-treatment numbers after 4 days and recovers fully by 10 days; peripheral (lymph nodes and spleen) (Treg) numbers recover to 8 and 6% by 2 days and fully recover to pre-treatment levels between 10 and 15 days after injection   (MGI Ref ID J:117743)
  • enlarged spleen
    • observed in 3-week old mutants treated daily with DT   (MGI Ref ID J:117743)
    • also seen in 3-month old adult mutants treated with DT   (MGI Ref ID J:117743)
  • increased dendritic cell number
    • numbers are elevated after Treg ablation   (MGI Ref ID J:117743)
  • behavior/neurological phenotype
  • bradykinesia
    • moribund newborns and adults treated with DT display lack of mobility   (MGI Ref ID J:117743)
  • liver/biliary system phenotype
  • liver inflammation
    • massive lymphocytic and mononuclear infiltrates in liver sinusoids seen at 3 weeks with DT treatment   (MGI Ref ID J:117743)
  • respiratory system phenotype
  • lung inflammation
    • massive lymphocytic and mononuclear infiltrates in lung interstitium seen at 3 weeks with DT treatment   (MGI Ref ID J:117743)
  • vision/eye phenotype
  • conjunctivitis
    • newborns treated with DT 12 hours after birth exhibit conjunctivitis; adults treated with DT show severe conjunctivitis   (MGI Ref ID J:117743)
  • integument phenotype
  • dermatitis
    • massive lymphocytic and mononuclear infiltrates in epidermis seen at 3 weeks with DT treatment   (MGI Ref ID J:117743)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Immunodeficiency
Inflammation
Lymphoid Tissue Defects

Research Tools
Fluorescent Proteins
Genetics Research
      Tissue/Cell Markers

GFP related

Research Tools
Fluorescent Proteins

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Foxp3tm3(DTR/GFP)Ayr
Allele Name targeted mutation 3, Alexander Y Rudensky
Allele Type Targeted
Common Name(s) Foxp3-DTR; Foxp3DTR; Foxp3tm3Ayr;
Mutation Made By Alexander Rudensky,   University of Washington
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Site of ExpressionDTR-EGFP expression is evident in regulatory T cells.
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Molecular Note A targeting vector was designed to insert an IRES-DTR-GFP with a floxed neo downstream of exon 11. [MGI Ref ID J:117743]
 
Gene Symbol and Name Foxp3, forkhead box P3
Chromosome X
Gene Common Name(s) AIID; DIETER; IPEX; JM2; PIDX; RGD1562112; XPID; scurfin; scurfy; sf;

Genotyping

Genotyping Information

Genotyping Protocols

Foxp3tm3Ayr alternate2,

SEPARATED MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kim JM; Rasmussen JP; Rudensky AY. 2007. Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice. Nat Immunol 8(2):191-7. [PubMed: 17136045]  [MGI Ref ID J:117743]

Additional References

Foxp3tm3(DTR/GFP)Ayr related

Aggarwal NR; Tsushima K; Eto Y; Tripathi A; Mandke P; Mock JR; Garibaldi BT; Singer BD; Sidhaye VK; Horton MR; King LS; D'Alessio FR. 2014. Immunological priming requires regulatory T cells and IL-10-producing macrophages to accelerate resolution from severe lung inflammation. J Immunol 192(9):4453-64. [PubMed: 24688024]  [MGI Ref ID J:209966]

Amado IF; Berges J; Luther RJ; Mailhe MP; Garcia S; Bandeira A; Weaver C; Liston A; Freitas AA. 2013. IL-2 coordinates IL-2-producing and regulatory T cell interplay. J Exp Med 210(12):2707-20. [PubMed: 24249704]  [MGI Ref ID J:208088]

Arpaia N; Campbell C; Fan X; Dikiy S; van der Veeken J; deRoos P; Liu H; Cross JR; Pfeffer K; Coffer PJ; Rudensky AY. 2013. Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation. Nature 504(7480):451-5. [PubMed: 24226773]  [MGI Ref ID J:207759]

Ballesteros-Tato A; Leon B; Graf BA; Moquin A; Adams PS; Lund FE; Randall TD. 2012. Interleukin-2 inhibits germinal center formation by limiting T follicular helper cell differentiation. Immunity 36(5):847-56. [PubMed: 22464171]  [MGI Ref ID J:187331]

Ballesteros-Tato A; Leon B; Lund FE; Randall TD. 2013. CD4+ T helper cells use CD154-CD40 interactions to counteract T reg cell-mediated suppression of CD8+ T cell responses to influenza. J Exp Med 210(8):1591-601. [PubMed: 23835849]  [MGI Ref ID J:202249]

Bettini ML; Pan F; Bettini M; Finkelstein D; Rehg JE; Floess S; Bell BD; Ziegler SF; Huehn J; Pardoll DM; Vignali DA. 2012. Loss of epigenetic modification driven by the Foxp3 transcription factor leads to regulatory T cell insufficiency. Immunity 36(5):717-30. [PubMed: 22579476]  [MGI Ref ID J:187320]

Bos PD; Plitas G; Rudra D; Lee SY; Rudensky AY. 2013. Transient regulatory T cell ablation deters oncogene-driven breast cancer and enhances radiotherapy. J Exp Med 210(11):2435-66. [PubMed: 24127486]  [MGI Ref ID J:206539]

Burzyn D; Kuswanto W; Kolodin D; Shadrach JL; Cerletti M; Jang Y; Sefik E; Tan TG; Wagers AJ; Benoist C; Mathis D. 2013. A special population of regulatory T cells potentiates muscle repair. Cell 155(6):1282-95. [PubMed: 24315098]  [MGI Ref ID J:205258]

Cascio JA; Haymaker CL; Divekar RD; Zaghouani S; Khairallah MT; Wan X; Rowland LM; Dhakal M; Chen W; Zaghouani H. 2013. Antigen-specific effector CD4 T lymphocytes school lamina propria dendritic cells to transfer innate tolerance. J Immunol 190(12):6004-14. [PubMed: 23686493]  [MGI Ref ID J:204839]

Crother TR; Schroder NW; Karlin J; Chen S; Shimada K; Slepenkin A; Alsabeh R; Peterson E; Arditi M. 2011. Chlamydia pneumoniae Infection Induced Allergic Airway Sensitization Is Controlled by Regulatory T-Cells and Plasmacytoid Dendritic Cells. PLoS One 6(6):e20784. [PubMed: 21695198]  [MGI Ref ID J:174290]

Darrasse-Jeze G; Deroubaix S; Mouquet H; Victora GD; Eisenreich T; Yao KH; Masilamani RF; Dustin ML; Rudensky A; Liu K; Nussenzweig MC. 2009. Feedback control of regulatory T cell homeostasis by dendritic cells in vivo. J Exp Med 206(9):1853-62. [PubMed: 19667061]  [MGI Ref ID J:152171]

Delgoffe GM; Woo SR; Turnis ME; Gravano DM; Guy C; Overacre AE; Bettini ML; Vogel P; Finkelstein D; Bonnevier J; Workman CJ; Vignali DA. 2013. Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis. Nature 501(7466):252-6. [PubMed: 23913274]  [MGI Ref ID J:201899]

Deppong CM; Bricker TL; Rannals BD; Van Rooijen N; Hsieh CS; Green JM. 2013. CTLA4Ig inhibits effector T cells through regulatory T cells and TGF-beta. J Immunol 191(6):3082-9. [PubMed: 23956428]  [MGI Ref ID J:205876]

Durant LR; Makris S; Voorburg CM; Loebbermann J; Johansson C; Openshaw PJ. 2013. Regulatory T cells prevent Th2 immune responses and pulmonary eosinophilia during respiratory syncytial virus infection in mice. J Virol 87(20):10946-54. [PubMed: 23926350]  [MGI Ref ID J:203872]

Ertelt JM; Buyukbasaran EZ; Jiang TT; Rowe JH; Xin L; Way SS. 2013. B7-1/B7-2 blockade overrides the activation of protective CD8 T cells stimulated in the absence of Foxp3+ regulatory T cells. J Leukoc Biol 94(2):367-76. [PubMed: 23744647]  [MGI Ref ID J:204423]

Fujisaki J; Wu J; Carlson AL; Silberstein L; Putheti P; Larocca R; Gao W; Saito TI; Lo Celso C; Tsuyuzaki H; Sato T; Cote D; Sykes M; Strom TB; Scadden DT; Lin CP. 2011. In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche. Nature 474(7350):216-9. [PubMed: 21654805]  [MGI Ref ID J:173452]

Gasteiger G; Hemmers S; Bos PD; Sun JC; Rudensky AY. 2013. IL-2-dependent adaptive control of NK cell homeostasis. J Exp Med 210(6):1179-87. [PubMed: 23650439]  [MGI Ref ID J:200963]

Gasteiger G; Hemmers S; Firth MA; Le Floc'h A; Huse M; Sun JC; Rudensky AY. 2013. IL-2-dependent tuning of NK cell sensitivity for target cells is controlled by regulatory T cells. J Exp Med 210(6):1167-78. [PubMed: 23650441]  [MGI Ref ID J:200961]

Goding SR; Wilson KA; Xie Y; Harris KM; Baxi A; Akpinarli A; Fulton A; Tamada K; Strome SE; Antony PA. 2013. Restoring Immune Function of Tumor-Specific CD4+ T Cells during Recurrence of Melanoma. J Immunol 190(9):4899-909. [PubMed: 23536636]  [MGI Ref ID J:195513]

Graham JB; Da Costa A; Lund JM. 2014. Regulatory T cells shape the resident memory T cell response to virus infection in the tissues. J Immunol 192(2):683-90. [PubMed: 24337378]  [MGI Ref ID J:207333]

Hindley JP; Jones E; Smart K; Bridgeman H; Lauder SN; Ondondo B; Cutting S; Ladell K; Wynn KK; Withers D; Price DA; Ager A; Godkin AJ; Gallimore AM. 2012. T-cell trafficking facilitated by high endothelial venules is required for tumor control after regulatory T-cell depletion. Cancer Res 72(21):5473-82. [PubMed: 22962270]  [MGI Ref ID J:191796]

Hurez V; Daniel BJ; Sun L; Liu AJ; Ludwig SM; Kious MJ; Thibodeaux SR; Pandeswara S; Murthy K; Livi CB; Wall S; Brumlik MJ; Shin T; Zhang B; Curiel TJ. 2012. Mitigating age-related immune dysfunction heightens the efficacy of tumor immunotherapy in aged mice. Cancer Res 72(8):2089-99. [PubMed: 22496463]  [MGI Ref ID J:185818]

Idoyaga J; Fiorese C; Zbytnuik L; Lubkin A; Miller J; Malissen B; Mucida D; Merad M; Steinman RM. 2013. Specialized role of migratory dendritic cells in peripheral tolerance induction. J Clin Invest :. [PubMed: 23298832]  [MGI Ref ID J:194499]

Irla M; Guerri L; Guenot J; Serge A; Lantz O; Liston A; Imhof BA; Palmer E; Reith W. 2012. Antigen recognition by autoreactive CD4(+) thymocytes drives homeostasis of the thymic medulla. PLoS One 7(12):e52591. [PubMed: 23300712]  [MGI Ref ID J:195832]

Kastenmuller W; Gasteiger G; Subramanian N; Sparwasser T; Busch DH; Belkaid Y; Drexler I; Germain RN. 2011. Regulatory T cells selectively control CD8+ T cell effector pool size via IL-2 restriction. J Immunol 187(6):3186-97. [PubMed: 21849683]  [MGI Ref ID J:179230]

Kim J; Lahl K; Hori S; Loddenkemper C; Chaudhry A; deRoos P; Rudensky A; Sparwasser T. 2009. Cutting edge: depletion of Foxp3+ cells leads to induction of autoimmunity by specific ablation of regulatory T cells in genetically targeted mice. J Immunol 183(12):7631-4. [PubMed: 19923467]  [MGI Ref ID J:157495]

Lanteri MC; O'Brien KM; Purtha WE; Cameron MJ; Lund JM; Owen RE; Heitman JW; Custer B; Hirschkorn DF; Tobler LH; Kiely N; Prince HE; Ndhlovu LC; Nixon DF; Kamel HT; Kelvin DJ; Busch MP; Rudensky AY; Diamond MS; Norris PJ. 2009. Tregs control the development of symptomatic West Nile virus infection in humans and mice. J Clin Invest 119(11):3266-77. [PubMed: 19855131]  [MGI Ref ID J:154592]

Leon B; Bradley JE; Lund FE; Randall TD; Ballesteros-Tato A. 2014. FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability. Nat Commun 5:3495. [PubMed: 24633065]  [MGI Ref ID J:210317]

Leonardo SM; De Santis JL; Malherbe LP; Gauld SB. 2012. Cutting edge: in the absence of regulatory T cells, a unique Th cell population expands and leads to a loss of B cell anergy. J Immunol 188(11):5223-6. [PubMed: 22544930]  [MGI Ref ID J:188735]

Liu K; Victora GD; Schwickert TA; Guermonprez P; Meredith MM; Yao K; Chu FF; Randolph GJ; Rudensky AY; Nussenzweig M. 2009. In Vivo Analysis of Dendritic Cell Development and Homeostasis. Science :. [PubMed: 19286519]  [MGI Ref ID J:147362]

Lu LF; Thai TH; Calado DP; Chaudhry A; Kubo M; Tanaka K; Loeb GB; Lee H; Yoshimura A; Rajewsky K; Rudensky AY. 2009. Foxp3-dependent microRNA155 confers competitive fitness to regulatory T cells by targeting SOCS1 protein. Immunity 30(1):80-91. [PubMed: 19144316]  [MGI Ref ID J:143720]

Lund JM; Hsing L; Pham TT; Rudensky AY. 2008. Coordination of early protective immunity to viral infection by regulatory T cells. Science 320(5880):1220-4. [PubMed: 18436744]  [MGI Ref ID J:136324]

Manrique SZ; Correa MA; Hoelzinger DB; Dominguez AL; Mirza N; Lin HH; Stein-Streilein J; Gordon S; Lustgarten J. 2011. Foxp3-positive macrophages display immunosuppressive properties and promote tumor growth. J Exp Med 208(7):1485-99. [PubMed: 21670203]  [MGI Ref ID J:176992]

Martinez RJ; Zhang N; Thomas SR; Nandiwada SL; Jenkins MK; Binstadt BA; Mueller DL. 2012. Arthritogenic self-reactive CD4+ T cells acquire an FR4hiCD73hi anergic state in the presence of Foxp3+ regulatory T cells. J Immunol 188(1):170-81. [PubMed: 22124124]  [MGI Ref ID J:180819]

Miyajima M; Chase CM; Alessandrini A; Farkash EA; Della Pelle P; Benichou G; Graham JA; Madsen JC; Russell PS; Colvin RB. 2011. Early acceptance of renal allografts in mice is dependent on foxp3(+) cells. Am J Pathol 178(4):1635-45. [PubMed: 21435448]  [MGI Ref ID J:169852]

Mortha A; Chudnovskiy A; Hashimoto D; Bogunovic M; Spencer SP; Belkaid Y; Merad M. 2014. Microbiota-dependent crosstalk between macrophages and ILC3 promotes intestinal homeostasis. Science 343(6178):1249288. [PubMed: 24625929]  [MGI Ref ID J:209599]

Mott KR; Gate D; Zandian M; Allen SJ; Rajasagi NK; van Rooijen N; Chen S; Arditi M; Rouse BT; Flavell RA; Town T; Ghiasi H. 2011. Macrophage IL-12p70 Signaling Prevents HSV-1-Induced CNS Autoimmunity Triggered by Autoaggressive CD4+ Tregs. Invest Ophthalmol Vis Sci 52(5):2321-33. [PubMed: 21220560]  [MGI Ref ID J:171538]

Ooi JD; Snelgrove SL; Engel DR; Hochheiser K; Ludwig-Portugall I; Nozaki Y; O'Sullivan KM; Hickey MJ; Holdsworth SR; Kurts C; Kitching AR. 2011. Endogenous foxp3(+) T-regulatory cells suppress anti-glomerular basement membrane nephritis. Kidney Int 79(9):977-86. [PubMed: 21248715]  [MGI Ref ID J:186874]

Pierson W; Cauwe B; Policheni A; Schlenner SM; Franckaert D; Berges J; Humblet-Baron S; Schonefeldt S; Herold MJ; Hildeman D; Strasser A; Bouillet P; Lu LF; Matthys P; Freitas AA; Luther RJ; Weaver CT; Dooley J; Gray DH; Liston A. 2013. Antiapoptotic Mcl-1 is critical for the survival and niche-filling capacity of Foxp3(+) regulatory T cells. Nat Immunol 14(9):959-65. [PubMed: 23852275]  [MGI Ref ID J:208243]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryThis mutant allele is located on the X chromosome. When maintaining a live colony, homozygous females may be bred with hemizygous males.
Mating SystemHomozygote x Hemizygote         (Female x Male)   31-DEC-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90MaleHemizygous for Foxp3tm3(DTR/GFP)Ayr  
$199.90FemaleHomozygous for Foxp3tm3(DTR/GFP)Ayr  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Foxp3tm3(DTR/GFP)Ayr x Hemizygous for Foxp3tm3(DTR/GFP)Ayr  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90MaleHemizygous for Foxp3tm3(DTR/GFP)Ayr  
$259.90FemaleHomozygous for Foxp3tm3(DTR/GFP)Ayr  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Foxp3tm3(DTR/GFP)Ayr x Hemizygous for Foxp3tm3(DTR/GFP)Ayr  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   005304 C57BL/6NJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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