Description

Strain Information

Former Names B6(C)-Cd1d1/Cd1d2tm1.2Aben/J    (Changed: 06-FEB-12 ) Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male)   01-OCT-12 Species laboratory mouse Generation F?+ (19-DEC-11) Generation Definitions   Donating Investigator Albert Bendelac ,   University of Chicago

Description
These mice carry a targeted knockout of the Cd1d1 (CD1d1 antigen) gene. Exons 2-6 were deleted, blocking expression of Cd1d1. Because the C57BL/6 Cd1d2 gene has a natural frameshift mutation that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface. Homozygotes are viable and fertile, and do not display any gross physical or behavioral abnormalities.

Development
Exons 2-6 and an FRT-flanked neomycin cassette were flanked by loxP sites in an embryonic stem (ES) cell line of C57BL/6 origin. The neomycin selection cassette was excised from the resulting mice through crosses with a C57BL/6 background FLP strain. Exons 2-6 were excised through crosses with B6.C-Tg(CMV-cre)1Cgn/J mice (see Stock No. 006954) .

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cd1d1

019418	B6(Cg)-Tg(Lck-Cd1d1)1Aben/J
016930	B6.129P2-Cd1d1tm2Aben/J
008881	B6.129S6-Cd1d1/Cd1d2tm1Spb/J
016929	C57BL/6-Cd1d1tm1.1Aben/J
006330	NOD.129S6(B6)-Cd1d1/Cd1d2tm1Spb/SbwJ

View Strains carrying other alleles of Cd1d1     (5 strains)

Additional Web Information

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd1d1^tm1.2Aben/Cd1d1^tm1.2Aben

        B6(C)-Cd1d1^tm1.2Aben/J

• mortality/aging
• increased susceptibility to bacterial infection induced morbidity/mortality
  • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
  • propranolol does not improve survival   (MGI Ref ID J:177038)
  • however, administration of an antibiotic improves survival   (MGI Ref ID J:177038)
• immune system phenotype
• abnormal CD4-positive T cell differentiation
  • following mouse focal cerebral ischemia, mice exhibit a switch in immune programming from Th1 to Th2 cytokines unlike in wild-type mice   (MGI Ref ID J:177038)
• abnormal T cell activation
  • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
• increased susceptibility to bacterial infection
  • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
• • increased susceptibility to bacterial infection induced morbidity/mortality
    • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
    • propranolol does not improve survival   (MGI Ref ID J:177038)
    • however, administration of an antibiotic improves survival   (MGI Ref ID J:177038)
• lung inflammation
  • following mouse focal cerebral ischemia, mice develop greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms compared with wild-type mice   (MGI Ref ID J:177038)
• respiratory system phenotype
• lung inflammation
  • following mouse focal cerebral ischemia, mice develop greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms compared with wild-type mice   (MGI Ref ID J:177038)
• nervous system phenotype
• increased susceptibility to ischemic brain injury
  • following mouse focal cerebral ischemia, mice exhibit increased bacterial counts in the blood and lungs, greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms, decreased T cell activation, a switch in immune programming from Th1 to Th2 cytokines, and increased mortality compared with wild-type mice   (MGI Ref ID J:177038)
  • propranolol does not improve survival   (MGI Ref ID J:177038)
  • however, infarct size is normal and administration of an antibiotic improves survival   (MGI Ref ID J:177038)
• homeostasis/metabolism phenotype
• increased susceptibility to ischemic brain injury
  • following mouse focal cerebral ischemia, mice exhibit increased bacterial counts in the blood and lungs, greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms, decreased T cell activation, a switch in immune programming from Th1 to Th2 cytokines, and increased mortality compared with wild-type mice   (MGI Ref ID J:177038)
  • propranolol does not improve survival   (MGI Ref ID J:177038)
  • however, infarct size is normal and administration of an antibiotic improves survival   (MGI Ref ID J:177038)
• cellular phenotype
• abnormal CD4-positive T cell differentiation
  • following mouse focal cerebral ischemia, mice exhibit a switch in immune programming from Th1 to Th2 cytokines unlike in wild-type mice   (MGI Ref ID J:177038)
• abnormal T cell activation
  • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
• hematopoietic system phenotype
• abnormal CD4-positive T cell differentiation
  • following mouse focal cerebral ischemia, mice exhibit a switch in immune programming from Th1 to Th2 cytokines unlike in wild-type mice   (MGI Ref ID J:177038)
• abnormal T cell activation
  • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      NK Cell Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cd1d1tm1.2Aben
Allele Name		targeted mutation 1.2, Albert Bendelac
Allele Type		Targeted (knock-out)
Common Name(s)		CD1.1 KO; Cd1d1/Cd1d2tm1.2Aben;
Strain of Origin		C57BL/6
Gene Symbol and Name		Cd1d1, CD1d1 antigen
Chromosome		3
Gene Common Name(s)		AI747460; CD1.1; CD1A; CD1a antigen; CD1d antigen; Cd1; Cd1a; Cd1d; Ly-38; R3; expressed sequence AI747460; lymphocyte antigen 38;
General Note		Because the C57BL/6 ES cells used to generate the allele contains a natural frameshift mutation in Cd1d2 that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface.
Molecular Note		Cre-mediated recombination removed exons 2 through 6 of and the neo cassette. [MGI Ref ID J:174415]

Genotyping

Genotyping Information

Genotyping Protocols

Cd1d1^tm1.2Abenalternate1, Separated PCR
Cd1d1/Cd1d2^tm1.2Aben, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bendelac A. 2011. Direct Data Submission 2011/08/15 MGI Direct Data Submission :.  [MGI Ref ID J:174415]

Bendelac A. 2011. Direct Data Submission 2011/08/08 MGI Direct Data Submission :.  [MGI Ref ID J:174336]

Additional References

Cd1d1^tm1.2Aben related

Tang ZH; Liang S; Potter J; Jiang X; Mao HQ; Li Z. 2013. Tim-3/galectin-9 regulate the homeostasis of hepatic NKT cells in a murine model of nonalcoholic Fatty liver disease. J Immunol 190(4):1788-96. [PubMed: 23296703]  [MGI Ref ID J:193017]

Wong CH; Jenne CN; Lee WY; Leger C; Kubes P. 2011. Functional innervation of hepatic iNKT cells is immunosuppressive following stroke. Science 334(6052):101-5. [PubMed: 21921158]  [MGI Ref ID J:177038]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)   01-OCT-12
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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