Strain Name:

B6(C)-Cd1d1tm1.2Aben/J

Stock Number:

017294

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Cd1d1 knock-out mice may be useful in the study of natural killer T (NKT) cells.

Description

Strain Information

Former Names B6(C)-Cd1d1/Cd1d2tm1.2Aben/J    (Changed: 06-FEB-12 )
Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-OCT-12
Specieslaboratory mouse
GenerationF?+f5 (19-DEC-11)
Generation Definitions
 
Donating Investigator Albert Bendelac ,   University of Chicago

Description
These mice carry a targeted knockout of the Cd1d1 (CD1d1 antigen) gene. Exons 2-6 were deleted, blocking expression of Cd1d1. Because the C57BL/6 Cd1d2 gene has a natural frameshift mutation that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface. Homozygotes are viable and fertile, and do not display any gross physical or behavioral abnormalities.

Development
Exons 2-6 and an FRT-flanked neomycin cassette were flanked by loxP sites in an embryonic stem (ES) cell line of C57BL/6 origin. The neomycin selection cassette was excised from the resulting mice through crosses with a C57BL/6 background FLP strain. Exons 2-6 were excised through crosses with B6.C-Tg(CMV-cre)1Cgn/J mice (see Stock No. 006954) .

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cd1d1
019418   B6(Cg)-Tg(Lck-Cd1d1)1Aben/J
016930   B6.129P2-Cd1d1tm2Aben/J
008881   B6.129S6-Cd1d1/Cd1d2tm1Spb/J
016929   C57BL/6-Cd1d1tm1.1Aben/J
006330   NOD.129S6(B6)-Cd1d1/Cd1d2tm1Spb/SbwJ
View Strains carrying other alleles of Cd1d1     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cd1d1tm1.2Aben/Cd1d1tm1.2Aben

        B6(C)-Cd1d1tm1.2Aben/J
  • mortality/aging
  • increased susceptibility to bacterial infection induced morbidity/mortality
    • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
    • propranolol does not improve survival   (MGI Ref ID J:177038)
    • however, administration of an antibiotic improves survival   (MGI Ref ID J:177038)
  • immune system phenotype
  • abnormal CD4-positive T cell differentiation
    • following mouse focal cerebral ischemia, mice exhibit a switch in immune programming from Th1 to Th2 cytokines unlike in wild-type mice   (MGI Ref ID J:177038)
  • abnormal T cell activation
    • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
  • increased susceptibility to bacterial infection
    • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
    • increased susceptibility to bacterial infection induced morbidity/mortality
      • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
      • propranolol does not improve survival   (MGI Ref ID J:177038)
      • however, administration of an antibiotic improves survival   (MGI Ref ID J:177038)
  • lung inflammation
    • following mouse focal cerebral ischemia, mice develop greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms compared with wild-type mice   (MGI Ref ID J:177038)
  • respiratory system phenotype
  • lung inflammation
    • following mouse focal cerebral ischemia, mice develop greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms compared with wild-type mice   (MGI Ref ID J:177038)
  • nervous system phenotype
  • increased susceptibility to ischemic brain injury
    • following mouse focal cerebral ischemia, mice exhibit increased bacterial counts in the blood and lungs, greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms, decreased T cell activation, a switch in immune programming from Th1 to Th2 cytokines, and increased mortality compared with wild-type mice   (MGI Ref ID J:177038)
    • propranolol does not improve survival   (MGI Ref ID J:177038)
    • however, infarct size is normal and administration of an antibiotic improves survival   (MGI Ref ID J:177038)
  • homeostasis/metabolism phenotype
  • increased susceptibility to ischemic brain injury
    • following mouse focal cerebral ischemia, mice exhibit increased bacterial counts in the blood and lungs, greater pulmonary damage and more prominent pulmonary neutrophil infiltration suggestive of earlier pneumonia-like symptoms, decreased T cell activation, a switch in immune programming from Th1 to Th2 cytokines, and increased mortality compared with wild-type mice   (MGI Ref ID J:177038)
    • propranolol does not improve survival   (MGI Ref ID J:177038)
    • however, infarct size is normal and administration of an antibiotic improves survival   (MGI Ref ID J:177038)
  • hematopoietic system phenotype
  • abnormal CD4-positive T cell differentiation
    • following mouse focal cerebral ischemia, mice exhibit a switch in immune programming from Th1 to Th2 cytokines unlike in wild-type mice   (MGI Ref ID J:177038)
  • abnormal T cell activation
    • following mouse focal cerebral ischemia   (MGI Ref ID J:177038)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      NK Cell Deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cd1d1tm1.2Aben
Allele Name targeted mutation 1.2, Albert Bendelac
Allele Type Targeted (Null/Knockout)
Common Name(s) CD1.1 KO; Cd1d1/Cd1d2tm1.2Aben;
Strain of OriginC57BL/6
Gene Symbol and Name Cd1d1, CD1d1 antigen
Chromosome 3
Gene Common Name(s) AI747460; CD1.1; CD1A; CD1a antigen; CD1d antigen; Cd1; Cd1a; Cd1d; Ly-38; R3; expressed sequence AI747460; lymphocyte antigen 38;
General Note Because the C57BL/6 ES cells used to generate the allele contains a natural frameshift mutation in Cd1d2 that prevents its surface expression, deletion of Cd1d1 is sufficient to abolish all CD1D protein from the cell surface.
Molecular Note Cre-mediated recombination removed exons 2 through 6 of and the neo cassette. [MGI Ref ID J:174415]

Genotyping

Genotyping Information

Genotyping Protocols

Cd1d1tm1.2Abenalternate1, Separated PCR
Cd1d1tm1.2Abenalternate1, Standard PCR
Cd1d1/Cd1d2tm1.2Aben, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bendelac A. 2011. Direct Data Submission 2011/08/15 MGI Direct Data Submission :.  [MGI Ref ID J:174415]

Bendelac A. 2011. Direct Data Submission 2011/08/08 MGI Direct Data Submission :.  [MGI Ref ID J:174336]

Additional References

Cd1d1tm1.2Aben related

Tang ZH; Liang S; Potter J; Jiang X; Mao HQ; Li Z. 2013. Tim-3/galectin-9 regulate the homeostasis of hepatic NKT cells in a murine model of nonalcoholic Fatty liver disease. J Immunol 190(4):1788-96. [PubMed: 23296703]  [MGI Ref ID J:193017]

Wong CH; Jenne CN; Lee WY; Leger C; Kubes P. 2011. Functional innervation of hepatic iNKT cells is immunosuppressive following stroke. Science 334(6052):101-5. [PubMed: 21921158]  [MGI Ref ID J:177038]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   01-OCT-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Cd1d1tm1.2Aben  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Cd1d1tm1.2Aben x Homozygous for Cd1d1tm1.2Aben  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Cd1d1tm1.2Aben  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Cd1d1tm1.2Aben x Homozygous for Cd1d1tm1.2Aben  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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