Strain Name:

STOCK Hmgn5tm1.1Mbus/J

Stock Number:

017298

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These mice possess loxP sites flanking exons 2 and 4 of the high mobility group nucleosomal binding domain 5 (Hmgn5) gene and may have applications in studies related to cancer progression.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names STOCK Hmgn5tm1Mbus/J    (Changed: 16-OCT-14 )
B6.Cg-Hmgn5tm1Mbus/J    (Changed: 05-JUN-13 )
B6.129S4-Hmgn5tm1Mbus/J    (Changed: 17-JUL-12 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?pN1
Generation Definitions
 
Donating Investigator Michael Bustin,   NIH

Description
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene, which code for the nucleosome binding domain. Female mice that are homozygous for this allele and male mice that are hemizygous for this X linked allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissue(s).

Development
A FRT site flanked targeting vector containing a NEO selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4, and another loxP site was inserted upstream of exon 2 of the targeted gene on the X chromosome. This construct was electroporated into (C57BL/6J x 129S4/SvJae)F1 derived v6.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on a C57BL/6J genetic background) expressing Flp recombinase. Mice that retained the floxed exons 2-4 were then bred to C57BL/6J mice for ten generations (and Flp transgene was removed) prior to sending males to The Jackson Laboratory Repository (see SNP results below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. This revealed 7 of 32 markers , one on chromosome 11 and two each on chromosomes 8, 13, and 15, that were not fixed for C57BL/6 allele-type (e.g.: segregating for 129S4 allele-type markers). These data suggest the mice sent to The Jackson Laboratory Repository were not sufficiently backcrossed onto the C57BL/6 genetic background to be considered congenic.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Hmgn5tm1.1Mbus/Hmgn5tm1.1Mbus

        involves: 129S4/SvJae * C57BL/6J
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable, fertile and normal in size and do not display any gross physical or behavioral abnormalities   (MGI Ref ID J:185263)

Hmgn5tm1.1Mbus/Y

        involves: 129S4/SvJae * C57BL/6J
  • normal phenotype
  • no abnormal phenotype detected
    • mice are viable, fertile and normal in size and do not display any gross physical or behavioral abnormalities   (MGI Ref ID J:185263)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Hmgn5tm1.1Mbus
Allele Name targeted mutation 1.1, Michael Bustin
Allele Type Targeted (Conditional ready (e.g. floxed), No functional change)
Strain of Origin(C57BL/6J x 129S4/SvJae)F1
Gene Symbol and Name Hmgn5, high-mobility group nucleosome binding domain 5
Chromosome X
Gene Common Name(s) GARP45; NBP-45; Nsbp1; nucleosome binding protein 1; nucleosome binding protein 45;
Molecular Note A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette and a loxP site was inserted downstream of exon 4. Flp-mediated recombination removed the neo cassette and left exons 2 through 4 floxed. [MGI Ref ID J:185263]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis,

Probe


Generic Cre Melt Curve Analysis, Melt Curve Analysis
Hmgn5tm1Mbus, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Rochman M; Taher L; Kurahashi T; Cherukuri S; Uversky VN; Landsman D; Ovcharenko I; Bustin M. 2011. Effects of HMGN variants on the cellular transcription profile. Nucleic Acids Res 39(10):4076-87. [PubMed: 21278158]  [MGI Ref ID J:182874]

Additional References

Hmgn5tm1.1Mbus related

Bustin M. 2012. Direct Data Submission MGI Direct Data Submission :.  [MGI Ref ID J:185263]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as female homozygotes and male hemizygotes. The gene is X-linked.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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