Description

Strain Information

Former Names B6.129S4-Hmgn5tm1Mbus/J    (Changed: 17-JUL-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Hemizygote         (Female x Male)   16-OCT-12 Species laboratory mouse Generation N10+N1F1 (16-JAN-13) Generation Definitions   Donating Investigator Michael Bustin,   NIH

Description
These mice possess loxP sites on either side of exons 2 through 4 of the targeted gene, which code for the nucleosome binding domain. Female mice that are homozygous for this allele and male mice that are hemizygous for this X linked allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 2 through 4 deleted in the cre-expressing tissue(s).

Development
A FRT site flanked targeting vector containing a NEO selection cassette was utilized in the construction of this mutant. This selection cassette and a loxP site was inserted downstream of exon 4, and another loxP site was inserted upstream of exon 2 of the targeted gene on the X chromosome. This construct was electroporated into (C57BL/6J x 129S4/SvJae)F1 derived v6.4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to transgenic mice (on a C57BL/6J genetic background) expressing Flp recombinase. Mice that retained the floxed exons 2-4 were then bred to C57BL/6J mice for ten generations (and Flp transgene was removed) prior to sending males to The Jackson Laboratory Repository (see SNP results below). Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis, with markers covering all 19 chromosomes and the X chromosome, was performed on the first generation rederived living colony at The Jackson Laboratory Repository. This revealed 7 of 32 markers , one on chromosome 11 and two each on chromosomes 8, 13, and 15, that were not fixed for C57BL/6 allele-type (e.g.: segregating for 129S4 allele-type markers). These data suggest the mice sent to The Jackson Laboratory Repository were not sufficiently backcrossed onto the C57BL/6 genetic background to be considered congenic.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Additional Web Information

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Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Hmgn5^tm1Mbus/Hmgn5⁺

        involves: 129S4/SvJae * C57BL/6J

• no phenotypic analysis
• *normal* no phenotypic analysis   (MGI Ref ID J:182874)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Hmgn5tm1Mbus
Allele Name		targeted mutation 1, Michael Bustin
Allele Type		Targeted (knock-out)
Strain of Origin		(C57BL/6J x 129S4/SvJae)F1
Gene Symbol and Name		Hmgn5, high-mobility group nucleosome binding domain 5
Chromosome		X
Gene Common Name(s)		GARP45; Hmgn5l1; NBP-45; NSBP1; Nsbp1; nucleosome binding protein 1; nucleosome binding protein 45;
Molecular Note		A FRT site flanked targeting vector containing NEO selection cassette was utilized in the construction of this mutant. This selection cassette and aloxP site was inserted downstream of exon 4 of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the neo cassette. [MGI Ref ID J:185263]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, Melt Curve Analysis
Hmgn5^tm1Mbus, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Rochman M; Taher L; Kurahashi T; Cherukuri S; Uversky VN; Landsman D; Ovcharenko I; Bustin M. 2011. Effects of HMGN variants on the cellular transcription profile. Nucleic Acids Res 39(10):4076-87. [PubMed: 21278158]  [MGI Ref ID J:182874]

Additional References

Hmgn5^tm1Mbus related

Bustin M. 2012. Direct Data Submission MGI Direct Data Submission :.  [MGI Ref ID J:185263]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as female homozygotes and male hemizygotes. The gene is X-linked.
Mating System	Heterozygote x Hemizygote         (Female x Male)   16-OCT-12
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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