Strain Name:

STOCK Neurog1tm1And/J

Stock Number:

017306

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In this strain, a green fluorescent protein (GFP) sequence fused to a neomycin resistance (neo) cassette replaces the coding exon of the neurogenin 1 (Neurog1) gene, abolishing gene function. GFP does not produce a fluorescent signal. These mice may be useful for studying neurogenesis in many parts of the nervous system.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator David J Anderson,   California Institute of Technology, HHMI

Description
In this strain, a green fluorescent protein (GFP) sequence fused to a neomycin resistance (neo) cassette replaces the coding exon of the neurogenin 1 (Neurog1) gene, abolishing gene function. Heterozygotes are viable and fertile. NEUROG1 is a neural-specific basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. NEUROG1 is also required for the formation of TrkA-expressing sensory neurons in the dorsal root ganglia that are involved in sensing pain, itch and temperatures. NEUROG1 is involved in neurogenesis and neuronal differentiation in the spinal cord and specific regions in the brain. In this strain, homozygotes lacking NEUROG1 fail to generate the proximal subset of cranial sensory neurons, such as neurons in the trigeminal, vestibulo-cochlear, superior, and jugular ganglia. These homozygous pups die within a day of birth due to inability to feed. GFP does not produce a fluorescent signal. These mice may be useful for studying neurogenesis in many parts of the nervous system.

Development
A targeting vector was designed to replace the coding exon of the neurogenin 1 (Neurog1) gene with a green fluorescent protein (GFP) sequence fused to a neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprt+-derived AB-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females and subsequently to ICR outbred mice. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Neurog1
008529   B6;129P-Tg(Neurog1-cre/ERT2)1Good/J
012859   STOCK Tg(Neurog1-cre)1Jejo/J
View Strains carrying other alleles of Neurog1     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Neurog1tm1And/Neurog1tm1And

        involves: 129S7/SvEvBrd * C57BL/6
  • mortality/aging
  • complete neonatal lethality
    • homozygotes die within 12 hours of birth   (MGI Ref ID J:46668)
  • behavior/neurological phenotype
  • abnormal suckling behavior
    • failed to suckle   (MGI Ref ID J:46668)
  • nervous system phenotype
  • abnormal cranial ganglia morphology   (MGI Ref ID J:46668)
    • abnormal superior glossopharyngeal ganglion morphology
      • superior ganglion lost after E10.5   (MGI Ref ID J:46668)
    • abnormal superior vagus ganglion morphology
      • jugular ganglion lost after E10.5   (MGI Ref ID J:46668)
    • absent trigeminal ganglion
      • absent at E10.5   (MGI Ref ID J:46668)
  • abnormal cranial nerve morphology   (MGI Ref ID J:46668)
    • abnormal accessory nerve morphology
      • lost at some time after E10.5 (either the nerve or its ganglion)   (MGI Ref ID J:46668)
    • abnormal trigeminal nerve morphology
      • truncated at E10.5   (MGI Ref ID J:46668)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Neurog1tm1And
Allele Name targeted mutation 1, David J Anderson
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) Ngn1GFP; ngn1-;
Mutation Made By David Anderson,   California Institute of Technology, HHMI
Strain of Origin129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Neurog1, neurogenin 1
Chromosome 13
Gene Common Name(s) AKA; Math4C; NEUROD3; Neurod3; Ngn1; bHLHa6; neurogenic differentiation 3; neurogenin;
Molecular Note The exon containing the entire coding region of the gene was replaced with a GFP-PGK-neo cassette via homologous recombination. In situ hybridization analysis of E10.5 embryos demonstrated the absence of gene expression in homozygous mutants. GFP transcripts were detected in mutant animals in a pattern indistinguishable from the endogenous mRNA but no fluorescent signal could be detected. [MGI Ref ID J:46668]

Genotyping

Genotyping Information

Genotyping Protocols

Neurog1tm1And, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ma Q; Chen Z; del Barco Barrantes I; de la Pompa JL; Anderson DJ. 1998. neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia. Neuron 20(3):469-82. [PubMed: 9539122]  [MGI Ref ID J:46668]

Additional References

Neurog1tm1And related

Azim E; Jabaudon D; Fame RM; Macklis JD. 2009. SOX6 controls dorsal progenitor identity and interneuron diversity during neocortical development. Nat Neurosci 12(10):1238-47. [PubMed: 19657336]  [MGI Ref ID J:154509]

Bachy I; Franck MC; Li L; Abdo H; Pattyn A; Ernfors P. 2011. The transcription factor Cux2 marks development of an A-delta sublineage of TrkA sensory neurons. Dev Biol 360(1):77-86. [PubMed: 21945863]  [MGI Ref ID J:178481]

Bluske KK; Vue TY; Kawakami Y; Taketo MM; Yoshikawa K; Johnson JE; Nakagawa Y. 2012. beta-Catenin signaling specifies progenitor cell identity in parallel with Shh signaling in the developing mammalian thalamus. Development 139(15):2692-702. [PubMed: 22745311]  [MGI Ref ID J:185651]

Bourane S; Garces A; Venteo S; Pattyn A; Hubert T; Fichard A; Puech S; Boukhaddaoui H; Baudet C; Takahashi S; Valmier J; Carroll P. 2009. Low-threshold mechanoreceptor subtypes selectively express MafA and are specified by Ret signaling. Neuron 64(6):857-70. [PubMed: 20064392]  [MGI Ref ID J:157721]

Britz O; Mattar P; Nguyen L; Langevin LM; Zimmer C; Alam S; Guillemot F; Schuurmans C. 2006. A role for proneural genes in the maturation of cortical progenitor cells. Cereb Cortex 16 Suppl 1:i138-51. [PubMed: 16766700]  [MGI Ref ID J:174488]

Cau E; Casarosa S; Guillemot F. 2002. Mash1 and Ngn1 control distinct steps of determination and differentiation in the olfactory sensory neuron lineage. Development 129(8):1871-80. [PubMed: 11934853]  [MGI Ref ID J:75936]

Cheng LE; Reed RR. 2007. Zfp423/OAZ participates in a developmental switch during olfactory neurogenesis. Neuron 54(4):547-57. [PubMed: 17521568]  [MGI Ref ID J:122275]

Dixit R; Wilkinson G; Cancino GI; Shaker T; Adnani L; Li S; Dennis D; Kurrasch D; Chan JA; Olson EC; Kaplan DR; Zimmer C; Schuurmans C. 2014. Neurog1 and Neurog2 control two waves of neuronal differentiation in the piriform cortex. J Neurosci 34(2):539-53. [PubMed: 24403153]  [MGI Ref ID J:205579]

Fode C; Ma Q; Casarosa S; Ang SL; Anderson DJ; Guillemot F. 2000. A role for neural determination genes in specifying the dorsoventral identity of telencephalic neurons. Genes Dev 14(1):67-80. [PubMed: 10640277]  [MGI Ref ID J:59428]

Gowan K; Helms AW; Hunsaker TL; Collisson T; Ebert PJ; Odom R; Johnson JE. 2001. Crossinhibitory activities of ngn1 and math1 allow specification of distinct dorsal interneurons. Neuron 31(2):219-32. [PubMed: 11502254]  [MGI Ref ID J:70696]

Heng JI; Nguyen L; Castro DS; Zimmer C; Wildner H; Armant O; Skowronska-Krawczyk D; Bedogni F; Matter JM; Hevner R; Guillemot F. 2008. Neurogenin 2 controls cortical neuron migration through regulation of Rnd2. Nature 455(7209):114-8. [PubMed: 18690213]  [MGI Ref ID J:139577]

Henke RM; Meredith DM; Borromeo MD; Savage TK; Johnson JE. 2009. Ascl1 and Neurog2 form novel complexes and regulate Delta-like3 (Dll3) expression in the neural tube. Dev Biol 328(2):529-40. [PubMed: 19389376]  [MGI Ref ID J:149457]

Hippenmeyer S; Shneider NA; Birchmeier C; Burden SJ; Jessell TM; Arber S. 2002. A role for neuregulin1 signaling in muscle spindle differentiation. Neuron 36(6):1035-49. [PubMed: 12495620]  [MGI Ref ID J:80793]

Kele J; Simplicio N; Ferri AL; Mira H; Guillemot F; Arenas E; Ang SL. 2006. Neurogenin 2 is required for the development of ventral midbrain dopaminergic neurons. Development 133(3):495-505. [PubMed: 16410412]  [MGI Ref ID J:104524]

Kramer I; Sigrist M; de Nooij JC; Taniuchi I; Jessell TM; Arber S. 2006. A role for Runx transcription factor signaling in dorsal root ganglion sensory neuron diversification. Neuron 49(3):379-93. [PubMed: 16446142]  [MGI Ref ID J:106980]

Kriks S; Lanuza GM; Mizuguchi R; Nakafuku M; Goulding M. 2005. Gsh2 is required for the repression of Ngn1 and specification of dorsal interneuron fate in the spinal cord. Development 132(13):2991-3002. [PubMed: 15930101]  [MGI Ref ID J:98802]

Ma Q; Anderson DJ; Fritzsch B. 2000. Neurogenin 1 null mutant ears develop fewer, morphologically normal hair cells in smaller sensory epithelia devoid of innervation. J Assoc Res Otolaryngol 1(2):129-43. [PubMed: 11545141]  [MGI Ref ID J:96038]

Ma Q; Fode C; Guillemot F; Anderson DJ. 1999. Neurogenin1 and neurogenin2 control two distinct waves of neurogenesis in developing dorsal root ganglia. Genes Dev 13(13):1717-28. [PubMed: 10398684]  [MGI Ref ID J:56292]

Matei V; Pauley S; Kaing S; Rowitch D; Beisel KW; Morris K; Feng F; Jones K; Lee J; Fritzsch B. 2005. Smaller inner ear sensory epithelia in Neurog 1 null mice are related to earlier hair cell cycle exit. Dev Dyn 234(3):633-50. [PubMed: 16145671]  [MGI Ref ID J:102293]

Mattar P; Britz O; Johannes C; Nieto M; Ma L; Rebeyka A; Klenin N; Polleux F; Guillemot F; Schuurmans C. 2004. A screen for downstream effectors of Neurogenin2 in the embryonic neocortex. Dev Biol 273(2):373-89. [PubMed: 15328020]  [MGI Ref ID J:93141]

Mattar P; Langevin LM; Markham K; Klenin N; Shivji S; Zinyk D; Schuurmans C. 2008. Basic helix-loop-helix transcription factors cooperate to specify a cortical projection neuron identity. Mol Cell Biol 28(5):1456-69. [PubMed: 18160702]  [MGI Ref ID J:132652]

Oh WJ; Gu C. 2013. Establishment of neurovascular congruency in the mouse whisker system by an independent patterning mechanism. Neuron 80(2):458-69. [PubMed: 24139045]  [MGI Ref ID J:207212]

Parras CM; Schuurmans C; Scardigli R; Kim J; Anderson DJ; Guillemot F. 2002. Divergent functions of the proneural genes Mash1 and Ngn2 in the specification of neuronal subtype identity. Genes Dev 16(3):324-38. [PubMed: 11825874]  [MGI Ref ID J:74525]

Qian Y; Fritzsch B; Shirasawa S; Chen CL; Choi Y; Ma Q. 2001. Formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is dependent on homeodomain protein Rnx/Tlx3. Genes Dev 15(19):2533-45. [PubMed: 11581159]  [MGI Ref ID J:72062]

Raft S; Koundakjian EJ; Quinones H; Jayasena CS; Goodrich LV; Johnson JE; Segil N; Groves AK. 2007. Cross-regulation of Ngn1 and Math1 coordinates the production of neurons and sensory hair cells during inner ear development. Development 134(24):4405-15. [PubMed: 18039969]  [MGI Ref ID J:129203]

Sammeta N; Hardin DL; McClintock TS. 2010. Uncx regulates proliferation of neural progenitor cells and neuronal survival in the olfactory epithelium. Mol Cell Neurosci 45(4):398-407. [PubMed: 20692344]  [MGI Ref ID J:171312]

Scardigli R; Schuurmans C; Gradwohl G; Guillemot F. 2001. Crossregulation between Neurogenin2 and Pathways Specifying Neuronal Identity in the Spinal Cord. Neuron 31(2):203-17. [PubMed: 11502253]  [MGI Ref ID J:70697]

Schuurmans C; Armant O; Nieto M; Stenman JM; Britz O; Klenin N; Brown C; Langevin LM; Seibt J; Tang H; Cunningham JM; Dyck R; Walsh C; Campbell K; Polleux F; Guillemot F. 2004. Sequential phases of cortical specification involve Neurogenin-dependent and -independent pathways. EMBO J 23(14):2892-902. [PubMed: 15229646]  [MGI Ref ID J:93057]

Shaker T; Dennis D; Kurrasch DM; Schuurmans C. 2012. Neurog1 and Neurog2 coordinately regulate development of the olfactory system. Neural Dev 7:28. [PubMed: 22906231]  [MGI Ref ID J:187366]

Takano-Maruyama M; Chen Y; Gaufo GO. 2012. Differential contribution of Neurog1 and Neurog2 on the formation of cranial ganglia along the anterior-posterior axis. Dev Dyn 241(2):229-41. [PubMed: 22102600]  [MGI Ref ID J:179737]

Wang L; Bluske KK; Dickel LK; Nakagawa Y. 2011. Basal progenitor cells in the embryonic mouse thalamus - their molecular characterization and the role of neurogenins and Pax6. Neural Dev 6:35. [PubMed: 22077982]  [MGI Ref ID J:178621]

Weston MD; Pierce ML; Jensen-Smith HC; Fritzsch B; Rocha-Sanchez S; Beisel KW; Soukup GA. 2011. MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival. Dev Dyn 240(4):808-19. [PubMed: 21360794]  [MGI Ref ID J:169673]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Homozygous mice die within a day of birth due to inability to feed.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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