|In this strain, a green fluorescent protein (GFP) sequence fused to a neomycin resistance (neo) cassette replaces the coding exon of the neurogenin 1 (Neurog1) gene, abolishing gene function. GFP does not produce a fluorescent signal. These mice may be useful for studying neurogenesis in many parts of the nervous system.|
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1
Donating Investigator David J Anderson, California Institute of Technology, HHMI
In this strain, a green fluorescent protein (GFP) sequence fused to a neomycin resistance (neo) cassette replaces the coding exon of the neurogenin 1 (Neurog1) gene, abolishing gene function. Heterozygotes are viable and fertile. NEUROG1 is a neural-specific basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. NEUROG1 is also required for the formation of TrkA-expressing sensory neurons in the dorsal root ganglia that are involved in sensing pain, itch and temperatures. NEUROG1 is involved in neurogenesis and neuronal differentiation in the spinal cord and specific regions in the brain. In this strain, homozygotes lacking NEUROG1 fail to generate the proximal subset of cranial sensory neurons, such as neurons in the trigeminal, vestibulo-cochlear, superior, and jugular ganglia. These homozygous pups die within a day of birth due to inability to feed. GFP does not produce a fluorescent signal. These mice may be useful for studying neurogenesis in many parts of the nervous system.
A targeting vector was designed to replace the coding exon of the neurogenin 1 (Neurog1) gene with a green fluorescent protein (GFP) sequence fused to a neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprt+-derived AB-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females and subsequently to ICR outbred mice. These mice were maintained on a mixed background. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation to establish the colony.
|Wild-type from the colony|
|Considerations for Choosing Controls|
Strains carrying other alleles of Neurog1View Strains carrying other alleles of Neurog1 (2 strains)
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Neurog1tm1And/Neurog1tm1Andinvolves: 129S7/SvEvBrd * C57BL/6
- complete neonatal lethality
- homozygotes die within 12 hours of birth (MGI Ref ID J:46668)
- behavior/neurological phenotype
- abnormal suckling behavior
- failed to suckle (MGI Ref ID J:46668)
- nervous system phenotype
- abnormal cranial ganglia morphology (MGI Ref ID J:46668)
- abnormal cranial nerve morphology (MGI Ref ID J:46668)
- View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Developmental Biology Research
Embryonic Lethality (Homozygous)
|Allele Name||targeted mutation 1, David J Anderson|
|Allele Type||Targeted (Null/Knockout, Reporter)|
|Common Name(s)||Ngn1GFP; ngn1-;|
|Mutation Made By||David Anderson, California Institute of Technology, HHMI|
|Strain of Origin||129S7/SvEvBrd-Hprt<+>|
|Gene Symbol and Name||Neurog1, neurogenin 1|
|Gene Common Name(s)||AKA; Math4C; NEUROD3; Neurod3; Ngn1; bHLHa6; neurogenic differentiation 3; neurogenin;|
|Molecular Note||The exon containing the entire coding region of the gene was replaced with a GFP-PGK-neo cassette via homologous recombination. In situ hybridization analysis of E10.5 embryos demonstrated the absence of gene expression in homozygous mutants. GFP transcripts were detected in mutant animals in a pattern indistinguishable from the endogenous mRNA but no fluorescent signal could be detected. [MGI Ref ID J:46668]|
Ma Q; Chen Z; del Barco Barrantes I; de la Pompa JL; Anderson DJ. 1998. neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia. Neuron 20(3):469-82. [PubMed: 9539122] [MGI Ref ID J:46668]
Azim E; Jabaudon D; Fame RM; Macklis JD. 2009. SOX6 controls dorsal progenitor identity and interneuron diversity during neocortical development. Nat Neurosci 12(10):1238-47. [PubMed: 19657336] [MGI Ref ID J:154509]
Bachy I; Franck MC; Li L; Abdo H; Pattyn A; Ernfors P. 2011. The transcription factor Cux2 marks development of an A-delta sublineage of TrkA sensory neurons. Dev Biol 360(1):77-86. [PubMed: 21945863] [MGI Ref ID J:178481]
Bluske KK; Vue TY; Kawakami Y; Taketo MM; Yoshikawa K; Johnson JE; Nakagawa Y. 2012. beta-Catenin signaling specifies progenitor cell identity in parallel with Shh signaling in the developing mammalian thalamus. Development 139(15):2692-702. [PubMed: 22745311] [MGI Ref ID J:185651]
Bourane S; Garces A; Venteo S; Pattyn A; Hubert T; Fichard A; Puech S; Boukhaddaoui H; Baudet C; Takahashi S; Valmier J; Carroll P. 2009. Low-threshold mechanoreceptor subtypes selectively express MafA and are specified by Ret signaling. Neuron 64(6):857-70. [PubMed: 20064392] [MGI Ref ID J:157721]
Britz O; Mattar P; Nguyen L; Langevin LM; Zimmer C; Alam S; Guillemot F; Schuurmans C. 2006. A role for proneural genes in the maturation of cortical progenitor cells. Cereb Cortex 16 Suppl 1:i138-51. [PubMed: 16766700] [MGI Ref ID J:174488]
Cau E; Casarosa S; Guillemot F. 2002. Mash1 and Ngn1 control distinct steps of determination and differentiation in the olfactory sensory neuron lineage. Development 129(8):1871-80. [PubMed: 11934853] [MGI Ref ID J:75936]
Dixit R; Wilkinson G; Cancino GI; Shaker T; Adnani L; Li S; Dennis D; Kurrasch D; Chan JA; Olson EC; Kaplan DR; Zimmer C; Schuurmans C. 2014. Neurog1 and Neurog2 control two waves of neuronal differentiation in the piriform cortex. J Neurosci 34(2):539-53. [PubMed: 24403153] [MGI Ref ID J:205579]
Fode C; Ma Q; Casarosa S; Ang SL; Anderson DJ; Guillemot F. 2000. A role for neural determination genes in specifying the dorsoventral identity of telencephalic neurons. Genes Dev 14(1):67-80. [PubMed: 10640277] [MGI Ref ID J:59428]
Gowan K; Helms AW; Hunsaker TL; Collisson T; Ebert PJ; Odom R; Johnson JE. 2001. Crossinhibitory activities of ngn1 and math1 allow specification of distinct dorsal interneurons. Neuron 31(2):219-32. [PubMed: 11502254] [MGI Ref ID J:70696]
Heng JI; Nguyen L; Castro DS; Zimmer C; Wildner H; Armant O; Skowronska-Krawczyk D; Bedogni F; Matter JM; Hevner R; Guillemot F. 2008. Neurogenin 2 controls cortical neuron migration through regulation of Rnd2. Nature 455(7209):114-8. [PubMed: 18690213] [MGI Ref ID J:139577]
Henke RM; Meredith DM; Borromeo MD; Savage TK; Johnson JE. 2009. Ascl1 and Neurog2 form novel complexes and regulate Delta-like3 (Dll3) expression in the neural tube. Dev Biol 328(2):529-40. [PubMed: 19389376] [MGI Ref ID J:149457]
Hippenmeyer S; Shneider NA; Birchmeier C; Burden SJ; Jessell TM; Arber S. 2002. A role for neuregulin1 signaling in muscle spindle differentiation. Neuron 36(6):1035-49. [PubMed: 12495620] [MGI Ref ID J:80793]
Kele J; Simplicio N; Ferri AL; Mira H; Guillemot F; Arenas E; Ang SL. 2006. Neurogenin 2 is required for the development of ventral midbrain dopaminergic neurons. Development 133(3):495-505. [PubMed: 16410412] [MGI Ref ID J:104524]
Kramer I; Sigrist M; de Nooij JC; Taniuchi I; Jessell TM; Arber S. 2006. A role for Runx transcription factor signaling in dorsal root ganglion sensory neuron diversification. Neuron 49(3):379-93. [PubMed: 16446142] [MGI Ref ID J:106980]
Kriks S; Lanuza GM; Mizuguchi R; Nakafuku M; Goulding M. 2005. Gsh2 is required for the repression of Ngn1 and specification of dorsal interneuron fate in the spinal cord. Development 132(13):2991-3002. [PubMed: 15930101] [MGI Ref ID J:98802]
Ma Q; Anderson DJ; Fritzsch B. 2000. Neurogenin 1 null mutant ears develop fewer, morphologically normal hair cells in smaller sensory epithelia devoid of innervation. J Assoc Res Otolaryngol 1(2):129-43. [PubMed: 11545141] [MGI Ref ID J:96038]
Ma Q; Fode C; Guillemot F; Anderson DJ. 1999. Neurogenin1 and neurogenin2 control two distinct waves of neurogenesis in developing dorsal root ganglia. Genes Dev 13(13):1717-28. [PubMed: 10398684] [MGI Ref ID J:56292]
Matei V; Pauley S; Kaing S; Rowitch D; Beisel KW; Morris K; Feng F; Jones K; Lee J; Fritzsch B. 2005. Smaller inner ear sensory epithelia in Neurog 1 null mice are related to earlier hair cell cycle exit. Dev Dyn 234(3):633-50. [PubMed: 16145671] [MGI Ref ID J:102293]
Mattar P; Britz O; Johannes C; Nieto M; Ma L; Rebeyka A; Klenin N; Polleux F; Guillemot F; Schuurmans C. 2004. A screen for downstream effectors of Neurogenin2 in the embryonic neocortex. Dev Biol 273(2):373-89. [PubMed: 15328020] [MGI Ref ID J:93141]
Mattar P; Langevin LM; Markham K; Klenin N; Shivji S; Zinyk D; Schuurmans C. 2008. Basic helix-loop-helix transcription factors cooperate to specify a cortical projection neuron identity. Mol Cell Biol 28(5):1456-69. [PubMed: 18160702] [MGI Ref ID J:132652]
Parras CM; Schuurmans C; Scardigli R; Kim J; Anderson DJ; Guillemot F. 2002. Divergent functions of the proneural genes Mash1 and Ngn2 in the specification of neuronal subtype identity. Genes Dev 16(3):324-38. [PubMed: 11825874] [MGI Ref ID J:74525]
Qian Y; Fritzsch B; Shirasawa S; Chen CL; Choi Y; Ma Q. 2001. Formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is dependent on homeodomain protein Rnx/Tlx3. Genes Dev 15(19):2533-45. [PubMed: 11581159] [MGI Ref ID J:72062]
Raft S; Koundakjian EJ; Quinones H; Jayasena CS; Goodrich LV; Johnson JE; Segil N; Groves AK. 2007. Cross-regulation of Ngn1 and Math1 coordinates the production of neurons and sensory hair cells during inner ear development. Development 134(24):4405-15. [PubMed: 18039969] [MGI Ref ID J:129203]
Sammeta N; Hardin DL; McClintock TS. 2010. Uncx regulates proliferation of neural progenitor cells and neuronal survival in the olfactory epithelium. Mol Cell Neurosci 45(4):398-407. [PubMed: 20692344] [MGI Ref ID J:171312]
Scardigli R; Schuurmans C; Gradwohl G; Guillemot F. 2001. Crossregulation between Neurogenin2 and Pathways Specifying Neuronal Identity in the Spinal Cord. Neuron 31(2):203-17. [PubMed: 11502253] [MGI Ref ID J:70697]
Schuurmans C; Armant O; Nieto M; Stenman JM; Britz O; Klenin N; Brown C; Langevin LM; Seibt J; Tang H; Cunningham JM; Dyck R; Walsh C; Campbell K; Polleux F; Guillemot F. 2004. Sequential phases of cortical specification involve Neurogenin-dependent and -independent pathways. EMBO J 23(14):2892-902. [PubMed: 15229646] [MGI Ref ID J:93057]
Takano-Maruyama M; Chen Y; Gaufo GO. 2012. Differential contribution of Neurog1 and Neurog2 on the formation of cranial ganglia along the anterior-posterior axis. Dev Dyn 241(2):229-41. [PubMed: 22102600] [MGI Ref ID J:179737]
Wang L; Bluske KK; Dickel LK; Nakagawa Y. 2011. Basal progenitor cells in the embryonic mouse thalamus - their molecular characterization and the role of neurogenins and Pax6. Neural Dev 6:35. [PubMed: 22077982] [MGI Ref ID J:178621]
Weston MD; Pierce ML; Jensen-Smith HC; Fritzsch B; Rocha-Sanchez S; Beisel KW; Soukup GA. 2011. MicroRNA-183 family expression in hair cell development and requirement of microRNAs for hair cell maintenance and survival. Dev Dyn 240(4):808-19. [PubMed: 21360794] [MGI Ref ID J:169673]
Animal Health ReportsProduction of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664). Homozygous mice die within a day of birth due to inability to feed.
|Pricing for USA, Canada and Mexico shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $2525.00
At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
|Pricing for International shipping destinations|
Cryopreserved Mice - Ready for Recovery
Price (US dollars $) Cryorecovery* $3283.00
Cryorecovery - Standard.
Progeny testing is not required.
|Wild-type from the colony|
|Considerations for Choosing Controls|
|Control Pricing Information for Genetically Engineered Mutant Strains.|
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.