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| These NOD background mice carry an autoreactive regulatory T cell receptor specific for insulin β chain peptide that renders them completely protected from diabetes development. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Li Wen, Yale School of Medicine Description
In addition to its biochemical functions, insulin can play an immunological role, acting as an autoantigen in human type I diabetes. In the NOD mouse model, many islet-reactive T cells invading the islet are insulin-specific. Studies suggest that insulin is also capable of generating a diabetes-protective immune response.A regulatory/immunosuppressive T cell line (designated 2H6) was isolated from the pancreatic lymph nodes of a diabetic NOD mouse. These cells specifically recognize the insulin β chain peptide 12-25 or 9-23, secrete IFNG and TGFB, and have the ability to block both the adoptive transfer of diabetes in NOD-scid recipients and the spontaneous development of diabetes in NOD mice.
Based on this cell line, a transgenic strain that expresses the autoreactive 2H6 Vα6/Vβ14 regulatory T cell receptor was created on the NOD genetic background. Expression of the transgene blocks CD8+ T cell development. These mice are completely protected from diabetes development and upon binding express significant amounts of IFNG and TGFB. Cells from these mice can differentiate into potent regulatory cells that can protect NOD mice from the aggressive effects of spleen cells transferred from diabetic mice and diabetogenic cells from BDC2.5 transgenic mice.
No phenotypic differences between hemizygotes and homozygotes have been found.
This strain has been useful in studies of regulatory T cell (Treg) function and in the development of therapies for autoimmune disorders.
Development
The donating laboratory isolated and cloned a regulatory/immunosuppressive T cell line (designated 2H6) from the pancreatic lymph nodes of a diabetic NOD mouse that specifically recognizes the insulin B chain peptide 12-25 or 9-23. 2H6 regulatory TCRα (Vα6 (Vα6/Jα45/Cα)) and TCRβ (Vβ14 (Vβ14/Dβ1.1Jβ2.3Cβ2)) constructs were directly coinjected into fertilized NOD oocytes to create a transgenic line. This strain was maintained on an NOD genetic background by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (45 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM)
resistant
Immunology, Inflammation and Autoimmunity Research
Autoimmunity
Type 1 Diabetes
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(TcraTcrb)2H6Lwn | ||
|---|---|---|---|
| Allele Name | transgene insertion 2H6, Li Wen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | 2H6 TCR transgenic; | ||
| Strain of Origin | NOD | ||
| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Molecular Note | The transgene contains the cloned regulatory/immunosuppressive T cell line (designated 2H6) from the pancreatic lymph nodes of a diabetic NOD mouse that specifically recognizes the insulin B chain peptide 12-25 or 9-23 (2H6 regulatory TCRalpha (Valpha6 (Valpha6/Jalpha45/Calpha)) and TCRbeta (Vbeta14 (Vbeta14/Dbeta1.1Jbeta2.3Cbeta2))). [MGI Ref ID J:108964] | ||
References
References provided by MGI
Selected Reference(s)
Du W; Wong FS; Li MO; Peng J; Qi H; Flavell RA; Sherwin R; Wen L. 2006. TGF-beta signaling is required for the function of insulin-reactive T regulatory cells. J Clin Invest 116(5):1360-70. [PubMed: 16670772] [MGI Ref ID J:108964]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Homozygotes or hemizygotes may be bred to maintain a colony.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
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JAX® Mice, Products & Services Conditions of Use
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