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| This Myo-Tg mutant mouse strain overexpresses myotrophin in the heart resulting in cardiac hypertrophy, which progresses to heart failure. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names FVB-Tg(Myh6-Mtpn)4Ssen/J (Changed: 28-JUN-12 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F28pN1
Generation DefinitionsDonating Investigator Subha Sen, Cleveland Clinic Description
These transgenic mice express mouse myotrophin (Mtpn) under the direction of the mouse Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, promoter. Transgenic mice exhibit cardiac hypertrophy at 4 weeks of age, which progresses to chronic hypertrophy and heart failure by 32 to 36 weeks of age. Transgenic mice die of heart failure at 9-12 months of age. The phenotype mimics the symptoms of human cardiomyopathy and heart failure, and includes: pleural effusion, left ventricular hypertrophy, multiple focal fibrosis, myocyte necrosis, lethargy, edema, compromised cardiac function, and lack of alertness. The Donating Investigator reports that there is no phenotypic difference between hemizygotes and homozygotes. Mice homozygous for the transgenic insert are viable and fertile.Development
A transgenic construct containing the mouse myotrophin (Mtpn) gene, under the direction of the mouse Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, promoter was injected into fertilized FVB mouse eggs. Founder line 4 was subsequently established. The donating investigator reported that these mice were maintained on a FVB background (see SNP note below). Upon arrival at The Jackson Laboratory, the mice were crossed to FVB/NJ (Stock No. 001800) at least once to establish the colony.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. 3 of the 27 markers throughout the genome were segregating, suggesting a mixed genetic background.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Myh6
021577 B6;D2-Tg(Myh6*-mCherry)2Mik/J 002637 B6SJL-Tg(CAMalpha1b)7Wjk/J 021853 FVB-Tg(Myh6-mCherry/Map1lc3a)1Rag/J View Strains carrying other alleles of Myh6 (3 strains)
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Cardiovascular Research
Heart Abnormalities
Other
Internal/Organ Research
Heart Abnormalities
Research Tools
Cardiovascular Research
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Myh6-Mtpn)4Ssen | ||
|---|---|---|---|
| Allele Name | transgene insertion 4, Subha Sen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Myo-Tg; | ||
| Mutation Made By | Subha Sen, Cleveland Clinic | ||
| Strain of Origin | FVB | ||
| Expressed Gene | Mtpn, myotrophin, mouse, laboratory | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory | ||
| Molecular Note | Transgene construct contains the mouse myotrophin (Mtpn) gene, under the direction of the mouse Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, promoter. Four lines were generated (line 1, 2, 3, and 4). [MGI Ref ID J:89823] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Myh6-Mtpn)4Ssen, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Gupta S; Maitra R; Young D; Gupta A; Sen S. 2009. Silencing the myotrophin gene by RNA interference leads to the regression of cardiac hypertrophy. Am J Physiol Heart Circ Physiol 297(2):H627-36. [PubMed: 19502558] [MGI Ref ID J:151093]
Kumar S; Seqqat R; Chigurupati S; Kumar R; Baker KM; Young D; Sen S; Gupta S. 2011. Inhibition of nuclear factor kappaB regresses cardiac hypertrophy by modulating the expression of extracellular matrix and adhesion molecules. Free Radic Biol Med 50(1):206-15. [PubMed: 21047552] [MGI Ref ID J:167486]
Sarkar S; Leaman DW; Gupta S; Sil P; Young D; Morehead A; Mukherjee D; Ratliff N; Sun Y; Rayborn M; Hollyfield J; Sen S. 2004. Cardiac overexpression of myotrophin triggers myocardial hypertrophy and heart failure in transgenic mice. J Biol Chem 279(19):20422-34. [PubMed: 14970239] [MGI Ref ID J:89823]
Additional References
Tg(Myh6-Mtpn)4Ssen relatedDas B; Young D; Vasanji A; Gupta S; Sarkar S; Sen S. 2010. Influence of p53 in the transition of myotrophin-induced cardiac hypertrophy to heart failure. Cardiovasc Res 87(3):524-34. [PubMed: 20202977] [MGI Ref ID J:176103]
Gupta A; Gupta S; Young D; Das B; McMahon J; Sen S. 2010. Impairment of ultrastructure and cytoskeleton during progression of cardiac hypertrophy to heart failure. Lab Invest 90(4):520-30. [PubMed: 20157292] [MGI Ref ID J:158590]
Gupta S; Young D; Maitra RK; Gupta A; Popovic ZB; Yong SL; Mahajan A; Wang Q; Sen S. 2008. Prevention of cardiac hypertrophy and heart failure by silencing of NF-kappaB. J Mol Biol 375(3):637-49. [PubMed: 18037434] [MGI Ref ID J:166343]
Sarkar S; Chawla-Sarkar M; Young D; Nishiyama K; Rayborn ME; Hollyfield JG; Sen S. 2004. Myocardial cell death and regeneration during progression of cardiac hypertrophy to heart failure. J Biol Chem 279(50):52630-42. [PubMed: 15385543] [MGI Ref ID J:95179]
Young D; Popovic ZB; Jones WK; Gupta S. 2008. Blockade of NF-kappaB using IkappaB alpha dominant-negative mice ameliorates cardiac hypertrophy in myotrophin-overexpressed transgenic mice. J Mol Biol 381(3):559-68. [PubMed: 18620706] [MGI Ref ID J:179421]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as homozygotes. Transgenic mice exhibit cardiac hypertrophy at 4 weeks of age, which progresses to chronic hypertrophy and heart failure by 36 weeks of age. Transgenic mice die of heart failure at 9-12 months of age.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2085.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2710.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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