Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1 Generation Definitions   Donating Investigator Dr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Description
Approximately 75% of homozygotes die before birth of unknown causes. The surviving homozygous females are sterile. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot or real-time RT-PCR analysis of homozygous knockout ES cells lines produced from mutant mice. Homozygous embryos exhibit development delays as early as embryonic day 8.5, and loss of homozygous embryos occurs E11.5 to E18.5. Approximately 16% of homozygous embryos, at E10.5 or younger, are partially or completely outside the yolk sac. Embryonic primordial germ cell migration is mislocalized. A reduced number of gonocytes/germ cells in homozygotes is detectable as early as E11.5. Homozygous adult females have smaller size ovaries when compared to wildtype littermates. Ovaries from 5 to 8 week old homozygotes have few follicles, and in ovaries from homozygous females 10 weeks of age or older, no follicles are observed. Although, homozygous males are fertile, testes from homozygous males have some empty seminiferous tubules.

Development
A targeting vector containing a PGK-NEO cassette was used to disrupt 2 kb of sequence encoding both genes. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into B6D2F2 blastocysts. Male germline transmission of the mutation was confirmed. The mice were maintained on a mixed B6;129S4 background. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Mirc5^tm1Jae/Mirc5^tm1Jae

        involves: 129S4/SvJae * C57BL/6

• mortality/aging
• partial embryonic lethality during organogenesis
  • some die between E11.5 and E13.5   (MGI Ref ID J:175228)
• partial lethality throughout fetal growth and development
  • some die between E13.5 and E18.5   (MGI Ref ID J:175228)
• partial prenatal lethality
  • at E18.5 only 7% of mice are homozygous rather than the expected 25%   (MGI Ref ID J:175228)
  • death occurs between E11.5 and E18.5   (MGI Ref ID J:175228)
• reproductive system phenotype
• *normal* reproductive system phenotype
  • males are fertile   (MGI Ref ID J:175228)
• • abnormal ovarian follicle number
    • at 5-8 weeks of age a small number of follicles are occasionally seen   (MGI Ref ID J:175228)
  • • absent ovarian follicles
      • ovaries of females older than 10 weeks of age lack follicular structures   (MGI Ref ID J:175228)
    • decreased primordial ovarian follicle number
      • ovaries display very few primordial follicles at P5 and P10   (MGI Ref ID J:175228)
  • abnormal primordial germ cell migration
    • many mislocalized PGCs (located near the hindlimb buds and the base of the tail) are seen at E9.5   (MGI Ref ID J:175228)
    • ectopic PGCs are detected as early as E8.5 and as late as E11.5   (MGI Ref ID J:175228)
  • abnormal seminiferous tubule morphology
    • empty tubules are seen alongside tubules filled with germ cells at various stages of spermatogenesis   (MGI Ref ID J:175228)
  • decreased germ cell number   (MGI Ref ID J:175228)
  • • decreased male germ cell number
      • at E13.5 and P5   (MGI Ref ID J:175228)
      • however, gonocyte proliferation is seen at P5 and P10 in males   (MGI Ref ID J:175228)
    • decreased oocyte number
      • at P5 ovaries contained less than 20% as many oocytes as wild-type ovaries   (MGI Ref ID J:175228)
    • decreased primordial germ cell number
      • at E11.5, most males and females have less than 5% as many germ cells as found in wild-type littermate controls in the genital ridge   (MGI Ref ID J:175228)
      • at E13.5 the number of MVH+ germ cells is reduced in males and females   (MGI Ref ID J:175228)
  • female infertility   (MGI Ref ID J:175228)
  • small ovary
    • ovary volume is less than 20% that of wild-type controls in adult females   (MGI Ref ID J:175228)
• embryogenesis phenotype
• abnormal embryogenesis/ development
  • before E10.5 about 16% of embryos are located outside the yolk sac   (MGI Ref ID J:175228)
• • abnormal somite development
    • embryos with developmental delay have a reduced number of somites   (MGI Ref ID J:175228)
  • delayed chorioallantoic fusion
    • in embryos with developmental delay   (MGI Ref ID J:175228)
  • delayed embryo turning
    • in embryos with developmental delay   (MGI Ref ID J:175228)
  • delayed neural tube closure
    • in embryos with developmental delay   (MGI Ref ID J:175228)
  • embryonic growth retardation
    • about 40% of embryos display general developmental delay by E8.5   (MGI Ref ID J:175228)
• nervous system phenotype
• delayed neural tube closure
  • in embryos with developmental delay   (MGI Ref ID J:175228)
• growth/size phenotype
• embryonic growth retardation
  • about 40% of embryos display general developmental delay by E8.5   (MGI Ref ID J:175228)
• endocrine/exocrine gland phenotype
• abnormal ovarian follicle number
  • at 5-8 weeks of age a small number of follicles are occasionally seen   (MGI Ref ID J:175228)
• • absent ovarian follicles
    • ovaries of females older than 10 weeks of age lack follicular structures   (MGI Ref ID J:175228)
  • decreased primordial ovarian follicle number
    • ovaries display very few primordial follicles at P5 and P10   (MGI Ref ID J:175228)
• abnormal seminiferous tubule morphology
  • empty tubules are seen alongside tubules filled with germ cells at various stages of spermatogenesis   (MGI Ref ID J:175228)
• small ovary
  • ovary volume is less than 20% that of wild-type controls in adult females   (MGI Ref ID J:175228)
• cellular phenotype
• abnormal primordial germ cell migration
  • many mislocalized PGCs (located near the hindlimb buds and the base of the tail) are seen at E9.5   (MGI Ref ID J:175228)
  • ectopic PGCs are detected as early as E8.5 and as late as E11.5   (MGI Ref ID J:175228)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Embryonic Lethality (Homozygous)
      incomplete
Internal/Organ Defects
      gonads

Reproductive Biology Research
Developmental Defects Affecting Gonads
      germ cell deficient

Research Tools
Reproductive Biology Research
      male germ cells
      oocyte

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Mirc5tm1Jae
Allele Name		targeted mutation 1, Rudolf Jaenisch
Allele Type		Targeted (knock-out)
Common Name(s)		mir-290-295-; xmiR290 cluster -;
Mutation Made By		Lea Medeiros,   Whitehead Institute
Strain of Origin		(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name		Mirc5, microRNA cluster 5, including Mir290 through Mir295
Chromosome		7
Gene Common Name(s)		EEmiRC;
Molecular Note		The entire 2 kb cluster including Mir290, Mir291a, Mir292, Mir291b, Mir293, Mir294, and Mir295 was replaced with a pgk-neo cassette via homologous recombination. RT-PCR confirmed the absence of mature micro RNAs in homozygous ES cells. [MGI Ref ID J:175228]

Genotyping

Genotyping Information

Genotyping Protocols

Mirc5^tm1Jae, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Medeiros LA; Dennis LM; Gill ME; Houbaviy H; Markoulaki S; Fu D; White AC; Kirak O; Sharp PA; Page DC; Jaenisch R. 2011. Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects. Proc Natl Acad Sci U S A 108(34):14163-8. [PubMed: 21844366]  [MGI Ref ID J:175228]

Additional References

Mirc5^tm1Jae related

Garcia PB; Cai A; Bates JG; Nolla H; Schlissel MS. 2012. miR290-5p/292-5p activate the immunoglobulin kappa locus in B cell development. PLoS One 7(8):e43805. [PubMed: 22928038]  [MGI Ref ID J:191683]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as heterozygotes. Approximately 75% of homozygotes die before birth of unknown causes. The surviving homozygous females are sterile.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

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General Terms and Conditions

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