Strain Name:

B6;129S4-Tet1tm1.1Jae/J

Stock Number:

017358

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Tet1 knock-out mice may be useful in studies of embryonic cell pluripotency and development.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHeterozygote x +/+ sibling         (Female x Male)   04-JUL-12
Mating System+/+ sibling x Heterozygote         (Female x Male)   04-JUL-12
Specieslaboratory mouse
Generationpn1+f3 (10-DEC-13)
Generation Definitions
 
Donating InvestigatorDr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Description
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA carrying exon 4 or protein) is detected by RT-PCR or Western blot analysis of embryonic stem cells homozygous for the targeted mutation. Some residual full-length transcript lacking exon 4 is detected. No truncated protein is detected by Western blot. mESC from homozygotes have a reduced level of 5-hydroxymethylcytosine (5hmC). Although homozygotes are viable, at birth, 75% of homozygotes have a smaller body size than wildtype controls. Homozygous embryos at E12.5 have fewer tail somite pairs than wildtype controls. Homozygous crosses produce very few pups.

Development
A targeting vector containing a FRT site flanked NEO cassette with a downstream loxP site was inserted upstream of exon 5. A second loxP site was inserted upstream of exon 4. The construct was electroporated into (C57BL/6 x 129S4/SvJae)F1 derived V6.5 embryonic stem (ES) cells, which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells that no longer contained exon 4 were injected into recipient blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Control Information

  Control
   Wild-type from the colony
   101043 B6129SF1/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tet1tm1.1Jae/Tet1tm1.1Jae

        involves: 129S4/SvJae * C57BL/6
  • reproductive system phenotype
  • decreased litter size
    • smaller than average litters   (MGI Ref ID J:175858)
  • growth/size/body phenotype
  • decreased birth body size
    • in 13 of 17 mice   (MGI Ref ID J:175858)
  • decreased body size
    • at 3 weeks but mice gain weight with age   (MGI Ref ID J:175858)
  • embryonic growth retardation
    • mild with variable size/development at E12.5   (MGI Ref ID J:175858)
  • immune system phenotype
  • decreased neutrophil cell number
  • embryogenesis phenotype
  • embryonic growth retardation
    • mild with variable size/development at E12.5   (MGI Ref ID J:175858)
  • hematopoietic system phenotype
  • decreased neutrophil cell number
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects
      Growth Defects (homozygous)

Research Tools
Developmental Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tet1tm1.1Jae
Allele Name targeted mutation 1.1, Rudolf Jaenisch
Allele Type Targeted (Null/Knockout)
Strain of Origin(C57BL/6 x 129S4/SvJae)F1
Gene Symbol and Name Tet1, tet methylcytosine dioxygenase 1
Chromosome 10
Gene Common Name(s) 2510010B09Rik; AA517754; BB001228; CXXC finger 6; CXXC6; Cxxc6; D10Ertd17e; DNA segment, Chr 10, ERATO Doi 17, expressed; LCX; RIKEN cDNA 2510010B09 gene; bA119F7.1; expressed sequence AA517754; expressed sequence BB001228; mKIAA1676;
Molecular Note A targeting vector containing a FRT site flanked neo cassette with a downstream loxP site was inserted upstream of exon 5. A second loxP site was inserted upstream of exon 4. Cre-mediated recombination removed the neo cassette and exon 4. The absence of protein expression was confirmed by western blot analysis on mouse embryonic fibroblasts. [MGI Ref ID J:175858]

Genotyping

Genotyping Information

Genotyping Protocols

Tet1tm1.1Jae, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Dawlaty MM; Ganz K; Powell BE; Hu YC; Markoulaki S; Cheng AW; Gao Q; Kim J; Choi SW; Page DC; Jaenisch R. 2011. Tet1 is dispensable for maintaining pluripotency and its loss is compatible with embryonic and postnatal development. Cell Stem Cell 9(2):166-75. [PubMed: 21816367]  [MGI Ref ID J:175858]

Additional References

Tet1tm1.1Jae related

Costa Y; Ding J; Theunissen TW; Faiola F; Hore TA; Shliaha PV; Fidalgo M; Saunders A; Lawrence M; Dietmann S; Das S; Levasseur DN; Li Z; Xu M; Reik W; Silva JC; Wang J. 2013. NANOG-dependent function of TET1 and TET2 in establishment of pluripotency. Nature 495(7441):370-4. [PubMed: 23395962]  [MGI Ref ID J:195146]

Dawlaty MM; Breiling A; Le T; Raddatz G; Barrasa MI; Cheng AW; Gao Q; Powell BE; Li Z; Xu M; Faull KF; Lyko F; Jaenisch R. 2013. Combined deficiency of Tet1 and Tet2 causes epigenetic abnormalities but is compatible with postnatal development. Dev Cell 24(3):310-23. [PubMed: 23352810]  [MGI Ref ID J:194056]

Huang H; Jiang X; Li Z; Li Y; Song CX; He C; Sun M; Chen P; Gurbuxani S; Wang J; Hong GM; Elkahloun AG; Arnovitz S; Wang J; Szulwach K; Lin L; Street C; Wunderlich M; Dawlaty M; Neilly MB; Jaenisch R; Yang FC; Mulloy JC; Jin P; Liu PP; Rowley JD; Xu M; He C; Chen J. 2013. TET1 plays an essential oncogenic role in MLL-rearranged leukemia. Proc Natl Acad Sci U S A 110(29):11994-9. [PubMed: 23818607]  [MGI Ref ID J:198803]

Rudenko A; Dawlaty MM; Seo J; Cheng AW; Meng J; Le T; Faull KF; Jaenisch R; Tsai LH. 2013. Tet1 is critical for neuronal activity-regulated gene expression and memory extinction. Neuron 79(6):1109-22. [PubMed: 24050401]  [MGI Ref ID J:201822]

Yamaguchi S; Shen L; Liu Y; Sendler D; Zhang Y. 2013. Role of Tet1 in erasure of genomic imprinting. Nature 504(7480):460-4. [PubMed: 24291790]  [MGI Ref ID J:207929]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as heterozygotes. Homozygous crosses produce very few pups.
Mating SystemHeterozygote x +/+ sibling         (Female x Male)   04-JUL-12
+/+ sibling x Heterozygote         (Female x Male)   04-JUL-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHeterozygous for Tet1tm1.1Jae  
Price per Pair (US dollars $)Pair Genotype
$399.80Heterozygous for Tet1tm1.1Jae x Heterozygous for Tet1tm1.1Jae  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHeterozygous for Tet1tm1.1Jae  
Price per Pair (US dollars $)Pair Genotype
$519.80Heterozygous for Tet1tm1.1Jae x Heterozygous for Tet1tm1.1Jae  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Wild-type from the colony
   101043 B6129SF1/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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