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| These OVE2447A mice harbor a mutation created by random insertion of the SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223). Using inverse PCR analysis, the transgene integration site was identified in intron 3 of the cramped-like (Drosophila) gene (Cramp1l) on chromosome 17. The donating investigator reports the phenotype of homozygous mice as: cleft palate. | |||||||||||||||||||
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Transposon Induced Mutation; Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1F1pN1
Generation DefinitionsDonating Investigator Paul A Overbeek, Baylor College of Medicine Description
These OVE2447A (OVE#2447A) mice harbor a mutation created by random insertion of the SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223). Using inverse PCR analysis, the lentiviral integration site was identified in intron 3 of the cramped-like [Drosophila] gene (Cramp1l) on chromosome 17. The 5'-LTR is linked to the (-) strand of DNA at position 25,135,745 bp [NCB137/mm9; 5'-25,135,745(-)]. The rtTA is inserted in the sense orientation relative to the disrupted mouse gene. The donating investigator reports the phenotype of homozygous mice as: cleft palate.Development
A lentiviral transgenic approach was used to generate these mice. The SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223) was designed with a Sleeping Beauty (SB) transposon (containing a slice-acceptor::IRES::rtTA::polyA gene trap), a mouse tyrosinase minigene (Tyro), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The 1200 bp SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), an adenovirus splice acceptor, a stop sequence (3xSTOP), an internal ribosome entry site (IRES; from human X-chromosome-linked inhibitor of apoptosis (XIAP)), a sequence encoding an optimized form of reverse tetracycline controlled transactivator (rtTA2S; with stop codon), a human growth hormone polyA sequence, and a second IR/DR sequence. The IR/DR sequences are outward-facing (pointed away from the sa-IRES-rtTA-pA). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. This packaged lentiposon was injected subzonally (under the zona pellucida) into 1-8 cell stage C57BL/6 mouse embryos. Founder mice (F0) were black, so presence of the lentiviral transgene was determined by PCR. Most F0 mice had more than one lentiviral integration site. F0 mice were assigned an OVE number and then bred with FVB/N mice. Expression of the tyrosinase minigene results in melanin synthesis, so agouti offspring (F1) were bred with FVB/N mice. F2 mice with different coat colors were designated as subline A, B, C, etc., for each family. F2 mice were bred with FVB/N mice. F3 mice with different coat colors were designated as sublines A-1, A-2, etc., or B-1, B-2, etc. Breeding with FVB/N mice was continued until litters with only one or two different coat colors were obtained. Mice with identical coat colors were then inbred and assessed for the presence or absence of viable homozygotes. Hemizygous mice of line OVE2447A (OVE#2447A) exhibit medium brown coat color. Using inverse PCR analysis, the lentiviral integration site was identified in intron 3 of the cramped-like [Drosophila] gene (Cramp1l) on chromosome 17. The 5'-LTR is linked to the (-) strand of DNA at position 25,135,745 bp [NCB137/mm9; 5'-25,135,745(-)]. The rtTA is inserted in the sense orientation relative to the disrupted mouse gene. Transgenic males were sent to The Jackson Laboratory Repository Facebase collection. Upon arrival, these mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the live colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: models
View Facebase: models (58 strains)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/Cramp1lTgTn(sb-rtTA,Tyr)2447AOve
involves: C57BL/6 * FVB/NThis mouse can be used to support research in many areas including:
Developmental Biology Research
Craniofacial and Palate Defects
congenital cleft palate
Research Tools
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Cramp1lTgTn(sb-rtTA,Tyr)2447AOve | ||
|---|---|---|---|
| Allele Name | transgenic transposon lentiviral insertion 2447A, Paul A Overbeek | ||
| Allele Type | Transgenic (random, gene disruption) | ||
| Common Name(s) | OVE#2447A; OVE2447A; | ||
| Strain of Origin | C57BL/6 | ||
| Site of Expression | Mice homozygous for the transgene disruption exhibit cleft palate. | ||
| Gene Symbol and Name | Cramp1l, Crm, cramped-like (Drosophila) | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | 5830477H08Rik; AI256853; AI790734; HN1L; RIKEN cDNA 5830477H08 gene; T-complex expressed gene 4; TCE4; Tce4; expressed sequence AI256853; expressed sequence AI790734; mKIAA1426; | ||
| Molecular Note | A lentiviral transgenic approach was used to generate these mice. The SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223) was designed with a Sleeping Beauty (SB) transposon (containing a slice-acceptor::IRES::rtTA::polyA gene trap), a mouse tyrosinase minigene (Tyro), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The 1200 bp SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), an adenovirus splice acceptor, a stop sequence (3xSTOP), an internal ribosome entry site (IRES; from human X-chromosome-linked inhibitor of apoptosis (XIAP)), a sequence encoding a-n optimized form of reverse tetracycline controlled transactivator (rtTA2S; with stop codon), a human growth hormone polyA sequence, and a second IR/DR sequence. The IR/DR sequences are outward-facing (pointed away from the sa-IRES-rtTA-pA). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2447A, a single copy of the lentiposon inserted into intron 3 [NCBI37/mm9; 5'-25,135,745(-)] in the sense orientation. [MGI Ref ID J:175597] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cramp1lTgTn(sb-rtTA,Tyr)2447AOve-3', Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Cramp1lTgTn(sb-rtTA,Tyr)2447AOve relatedOverbeek PA. 2011. Direct Data Submission for Overbeek Lentiviral Transgenic Lines MGI Direct Data Submission :. [MGI Ref ID J:175597]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred together, bred with wildtype siblings, or bred with FVB/N inbred mice (Stock No. 001800).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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