Strain Name:

129S(B6)-Del(17Abcg1-Rrp1b)5Yey/J

Stock Number:

017440

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
This Df(17)4Yey/+ mutant strain contains one copy of mouse Chromosome 17 with the targeted sequence deleted between, and including, the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene and ribosomal RNA processing 1 homolog B (S. cerevisiae) (Rrp1b) gene. These mice may be useful for understanding developmental and cognitive disabilities associated with Monosomy 21.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names 129S(B6)-Del(17Abcg1-Rrp1b)4Yey/J    (Changed: 30-SEP-11 )
129S.129S7(B6)-Del(17Abcg1-Rrp1b)4Yey/J    (Changed: 16-SEP-11 )
Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
Generation+pN1
Generation Definitions
 
Donating Investigator Y. Eugene Yu,   Roswell Park Cancer Institute

Description
This Df(17)4Yey/+ mutant strain contains one copy of mouse Chromosome 17 with the targeted sequence deleted between, and including, the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene and ribosomal RNA processing 1 homolog B (S. cerevisiae) (Rrp1b) gene. Heterozygous mice are viable and fertile. This deleted region on the mouse chromosome is one of two regions orthologous to a deleted copy of human Chromosome 21 (Hsa21) which has been implicated in developmental cognitive disabilities like Down Syndrome (DS) and Monosomy 21. Dp(17)4Yey/+ lacks 19 genes on Chromosome 17 which share conserved synteny with the distal part of human 21q22.3, and is orthologous to genes on Hsa21. These mice exhibit Monosomy 21 related phenotypic defects including impaired contextual conditioning behavior, and abnormal spatial learning. These mice may be useful for understanding developmental and cognitive disabilities associated with Monosomy 21.

Development
Chromosome-engineering cassettes were inserted into mouse Chromosome 17 of 129S7/SvEvBrd-Hprt1b-m2-derived AB2.2 embryonic stem (ES) cells, bracketing a span of approximately 1.1 Mb between, and including, the ATP-binding cassette, sub-family G (WHITE), member 1 (Abcg1) gene and ribosomal RNA processing 1 homolog B (S. cerevisiae) (Rrp1b) gene. The cassette placed at the proximal locus was targeted upstream of Abcg1 and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. The cassette placed at the distal locus was targeted downstream of the Rrp1b gene and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231. After subsequent selection of recombinants by using hypoxanthine aminopterin thymidine (HAT) media, correctly targeted ES cells, containing one copy of mouse Chromosome 17 with the targeted sequence deleted (Del), were injected into C57BL/6J-Tyrc-Brd blastocysts. The resulting chimeric males were mated to C57BL/6J females and were subsequently backcrossed to 129/SvEv mice for at least 7 generations to produce a colony of Df(17)4Yey/+ mice. Upon arrival at The Jackson Laboratory these mice were bred to 129S1/SvImJ mice (Stock No. 002448) for at least one generation.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Del(17Abcg1-Rrp1b)5Yey/+

        129S.129S7-Del(17Abcg1-Rrp1b)5Yey
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice exhibit normal cued conditioning and pain sensitivity   (MGI Ref ID J:161398)
    • abnormal spatial learning
      • after training, mice take longer to find the hidden platform in a Morris water maze test than wild-type mice   (MGI Ref ID J:161398)
    • impaired contextual conditioning behavior
      • mice exhibit reduced freezing in a 24-h and 72-h context test compared with wild-type mice   (MGI Ref ID J:161398)
    • impaired swimming
      • mice swim slower than wild-type mice   (MGI Ref ID J:161398)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Del(17Abcg1-Rrp1b)5Yey
Allele Name deletion, Chr 17, Y Eugene Yu 5
Allele Type Targeted (other)
Common Name(s) Del(17)1Yey; Del(17Abcg1-Rrp1b)1Yey; Del4Yey; Df(17)1Yey; Df(Abcg1-Rrp1b)1Yey;
Strain of Origin129S7/SvEvBrd-Hprt
Gene Symbol and Name Del(17Abcg1-Rrp1b)5Yey, deletion, Chr 17, Y Eugene Yu 5
Chromosome 17
Gene Common Name(s) Del(17Abcg1-Rrp1b)1Yey; deletion, Chr 17, Y Eugene Yu 1;
General Note Phenotypic Similarity to Human Syndrome: Monosomy 21-associated mental retardation
Molecular Note Cre mediated chromosomal rearrangement between loxP sites in regions proximal to Abcg1 and distal to Rrp1b resulted in the deletion of the genes, including Abcg1 and Rrp1b, in this approximately 1.1 Mb region that is syntenic with the proximal part of human 21q22.3. [MGI Ref ID J:161398]

Genotyping

Genotyping Information

Genotyping Protocols

Del(17Abcg1-Rrp1b)5Yey alternate2, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Yu T; Clapcote SJ; Li Z; Liu C; Pao A; Bechard AR; Carattini-Rivera S; Matsui S; Roder JC; Baldini A; Mobley WC; Bradley A; Yu YE. 2010. Deficiencies in the region syntenic to human 21q22.3 cause cognitive deficits in mice. Mamm Genome 21(5-6):258-67. [PubMed: 20512340]  [MGI Ref ID J:161398]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype (non-carrier) mice from the colony or to 129S1/SvImJ inbred mice (Stock No. 002448).

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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