Strain Name:

B6.129S7-Shank3tm1Yhj/J

Stock Number:

017442

Order this mouse

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
In this strain, a neo cassette replaces exons 4-9 of the Shank3 gene. Homozygous mice display abnormal social behaviors, communication patterns, repetitive behaviors, learning and memory. These mice may be useful for studying behavioral abnormalities associated with autism spectrum disorder.

Description

Strain Information

Type Congenic; Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemWild-type x Heterozygote         (Female x Male)   09-OCT-12
Mating SystemHeterozygote x Wild-type         (Female x Male)   09-OCT-12
Specieslaboratory mouse
GenerationN7+pN1F2 (09-OCT-12)
Generation Definitions
 
Donating Investigator Yong-hui Jiang,   Duke University School of Medicine

Description
In this strain, a neo cassette replaces exons 4-9 of SH3/ankyrin domain 3 (Shank3) gene. SHANK3 is a synaptic scaffolding protein, expressed in the postsynaptic density (PSD) of excitatory synapses, which contains five intragenic promoters allowing for extensive alternative splicing. SHANK3 mutations have been identified in cases of intellectual disability such as autism spectrum disorder (ASD), Phelan-McDermid syndrome, and Schizophrenia. In these Shank3e4-9 mice, the neo cassette is inserted downstream of Promoter 2, making these mice deficient in Shank3a-b which are transcripts from Promoters 1-2. They do produce transcripts Shank3c-e from downstream Promoters 3-5. These mice display abnormal social behaviors, communication patterns, repetitive behaviors, learning and memory. Male mice also display severe motor coordination impairments. Shank3e4-9 mice also exhibit morphological alterations in dendritic spines and are deficient in long-term potentiation. Homozygotes are viable and fertile.These mice may be useful for studying behavioral abnormalities associated with ASD.

Development
A targeting vector was designed to replace exons 4-9 of SH3/ankyrin domain 3 (Shank3) gene with a neomycin resistance (neo) cassette. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2-derived AB2.2 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were bred to C57BL/6 females. These mice were backcrossed for at least 7 generations to C57BL/6J background. The donating investigator states that the naturally occurring Disc1 (Disrupted in Schizophrenia 1) mutation in the 129SvEv ES cells was bred out of this Shank3e4-9 line. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J (Stock No. 000664) for at least one generation.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Shank3
017889   B6(Cg)-Shank3tm1.1Bux/J
017890   B6(Cg)-Shank3tm1.2Bux/J
017688   B6.129-Shank3tm2Gfng/J
024033   B6.FVB-Tg(Shank3-EGFP)1Hzo/J
018398   STOCK Shank3tm1.1Pfw/J
View Strains carrying other alleles of Shank3     (5 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Autism
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Phelan-Mcdermid Syndrome   (SHANK3)
Schizophrenia 15; SCZD15   (SHANK3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Shank3tm1Yhj/Shank3tm1Yhj

        B6.129S7-Shank3tm1Yhj
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice exhibit normal prepulse inhibition, startle response, anxiety related behavior in a light-dark emergence test and elevated zero maze, novel object recognition, and social transmission of food preference   (MGI Ref ID J:173402)
    • abnormal motor capabilities/coordination/movement
      • mice exhibit reduced orienting to moving objects compared with wild-type mice   (MGI Ref ID J:173402)
      • mice exhibit decreased darting compared with wild-type mice   (MGI Ref ID J:173402)
      • abnormal gait
        • as detected by a treadscan gait analysis, mice exhibit increased stance and brake time and diagonal coupling ratio compared with wild-type mice   (MGI Ref ID J:173402)
      • decreased vertical activity
        • in male mice, but not female mice   (MGI Ref ID J:173402)
      • hypoactivity
        • in male mice, but not female mice   (MGI Ref ID J:173402)
      • impaired coordination
        • mice exhibit impaired climbing down a vertical pole compared with wild-type mice   (MGI Ref ID J:173402)
        • in a foot-misplacement test, mice exhibit increased foot-fault compared with wild-type mice   (MGI Ref ID J:173402)
        • male mice exhibit more severe impairments than female mice   (MGI Ref ID J:173402)
      • impaired righting response
        • in male mice tip-over in a vertical placement test compared with wild-type mice   (MGI Ref ID J:173402)
      • increased stereotypic behavior
        • in a hole-board test, mice exhibit more head pokes compared with wild-type mice   (MGI Ref ID J:173402)
        • when encountering a novel object in a familiar environment, mice exhibit stereotypic investigation and self-stimulatory behavior compared with wild-type mice   (MGI Ref ID J:173402)
    • abnormal motor learning
      • male mice exhibit impaired motor learning on a rotarod compared with wild-type mice   (MGI Ref ID J:173402)
      • however, female mice exhibit normal motor learning   (MGI Ref ID J:173402)
    • abnormal object recognition memory
      • mice exhibit decreased novel object preference compared with wild-type mice   (MGI Ref ID J:173402)
      • abnormal long term object recognition memory
        • during long term and remote memory tests, mice exhibit more object contacts than wild-type mice   (MGI Ref ID J:173402)
    • abnormal social investigation
      • mice fail to show social affiliation compared with wild-type mice   (MGI Ref ID J:173402)
      • mice exhibit reduced social behavior in a dyadic test and elicit less social interaction from C3H partner mice compared with wild-type mice   (MGI Ref ID J:173402)
      • mice exhibit reduced time sniffing compared with wild-type mice   (MGI Ref ID J:173402)
      • however, mice prefer interacting with a novel animal when forced to choose between two social stimuli in the social preference test   (MGI Ref ID J:173402)
    • abnormal spatial learning
      • in Morris water maze due to test familiarization or delayed learning, rather than specific sensory, motor, or motivational abnormalities   (MGI Ref ID J:173402)
    • abnormal vocalization
      • mice exhibit aberrant duration and frequency of ultrasonic vocalizations compared with wild-type mice   (MGI Ref ID J:173402)
    • increased grooming behavior   (MGI Ref ID J:173402)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • brain weight is normal at 2 to 4 months of age   (MGI Ref ID J:173402)
    • mice exhibit normal basal synaptic transmission   (MGI Ref ID J:173402)
    • abnormal dendritic spine morphology
      • at 4 weeks of age, spines are less dense and longer than in wild-type mice   (MGI Ref ID J:173402)
      • at 10 weeks, spine length is increased compared to in wild-type mice   (MGI Ref ID J:173402)
      • however, spine density at 10 weeks of age is normal   (MGI Ref ID J:173402)
    • decreased post-tetanic potentiation   (MGI Ref ID J:173402)
    • impaired synaptic plasticity
      • mice exhibit impaired hippocampal CA1 synaptic plasticity compared with wild-type mice   (MGI Ref ID J:173402)
    • reduced long term potentiation   (MGI Ref ID J:173402)
  • growth/size/body phenotype
  • increased body weight
    • slightly at 8 to 12 months   (MGI Ref ID J:173402)
    • however, body weight is normal at 3 to 4 months of age   (MGI Ref ID J:173402)
  • taste/olfaction phenotype
  • *normal* taste/olfaction phenotype
    • mice exhibit normal olfaction   (MGI Ref ID J:173402)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Neurodevelopmental Defects
      Autism

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Shank3tm1Yhj
Allele Name targeted mutation 1, Yong-hui Jiang
Allele Type Targeted
Common Name(s) Shank3e4-9;
Mutation Made By Yong-hui Jiang,   Duke University School of Medicine
Strain of Origin129S7/SvEvBrd-Hprt
Gene Symbol and Name Shank3, SH3/ankyrin domain gene 3
Chromosome 15
Gene Common Name(s) AI841104; DEL22q13.3; PROSAP2; PSAP2; SCZD15; SPANK-2; expressed sequence AI841104;
Molecular Note Exons 4 through 9, which encode the ankyrin repeats, were replaced with a neo cassette. The absence of the 190 kDa but presence of of the 170 kDa and 140 kDa peptides was confirmed by western blot analysis on brian extracts. The a and b isoforms are deleted. [MGI Ref ID J:173402]

Genotyping

Genotyping Information

Genotyping Protocols

Shank3tm1Yhjalternate1, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wang X; McCoy PA; Rodriguiz RM; Pan Y; Je HS; Roberts AC; Kim CJ; Berrios J; Colvin JS; Bousquet-Moore D; Lorenzo I; Wu G; Weinberg RJ; Ehlers MD; Philpot BD; Beaudet AL; Wetsel WC; Jiang YH. 2011. Synaptic dysfunction and abnormal behaviors in mice lacking major isoforms of Shank3. Hum Mol Genet 20(15):3093-108. [PubMed: 21558424]  [MGI Ref ID J:173402]

Additional References

Shank3tm1Yhj related

Jiang YH; Ehlers MD. 2013. Modeling autism by SHANK gene mutations in mice. Neuron 78(1):8-27. [PubMed: 23583105]  [MGI Ref ID J:197901]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice (Stock No. 000664).
Mating SystemWild-type x Heterozygote         (Female x Male)   09-OCT-12
Heterozygote x Wild-type         (Female x Male)   09-OCT-12
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Shank3tm1Yhj  
Price per Pair (US dollars $)Pair Genotype
$304.00Heterozygous for Shank3tm1Yhj x Wild-type for Shank3tm1Yhj  
$304.00Wild-type for Shank3tm1Yhj x Heterozygous for Shank3tm1Yhj  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Shank3tm1Yhj  
Price per Pair (US dollars $)Pair Genotype
$395.20Heterozygous for Shank3tm1Yhj x Wild-type for Shank3tm1Yhj  
$395.20Wild-type for Shank3tm1Yhj x Heterozygous for Shank3tm1Yhj  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)