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| These PP2A-A-alpha-E64G mice contain the amino acid mutation E64G in exon 3 of the A-alpha subunit gene (Ppp2r1a) of protein phosphatase 2A (PP2A). This mutation was detected in a human breast carcinoma. These mice may be useful for studying tumor suppression, development, and aging. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 26-MAR-12 Species laboratory mouse Generation N14pN1
Generation DefinitionsDonating Investigator Gernot Walter, UCSD Description
These PP2A-A-alpha-E64G mice contain the amino acid mutation E64G in exon 3 of the A-alpha subunit gene (Ppp2r1a) of protein phosphatase 2A (PP2A). This mutation was detected in a human breast carcinoma. Homozygotes are viable and fertile. PP2A holoenzyme is composed of a catalytic C subunit, a scaffolding A subunit, and a regulatory B subunit. It suppresses the development of lung cancer induced by the carcinogen benzopyrene. This tumor suppressor activity is p53-dependent. PP2A also prolongs the survival of mice expressing oncogenic K-rasG12D (v-Ki-ras2 Kirsten rat sarcoma). PP2A-A-alpha-E64G mice are a model for studying the tumor suppressor activity of B'-containing PP2A holoenzymes in mammary gland, lung, and other organs. The E64G mutation in this mouse strain renders the Aα subunit defective in binding regulatory B' subunits whereas binding of B and B'' subunits is normal. These mice may be useful for studying tumor suppression, development, and aging.Development
A targeting construct was designed to introduce two point mutations in codon 64, exon 3, of the A-alpha subunit gene (Ppp2r1a) of protein phosphatase 2A (PP2A) resulting in the amino acid substitution E64G discovered in a human breast carcinoma. A loxP-flanked neomycin (neo) resistance cassette was inserted downstream of exon 3. This targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells and correctly targeted ES cells were injected into blastocysts. Chimeric males were bred to female 129S mice and the resulting offspring were subsequently bred to mice carrying prion driven Cre-recombinase on a 129S background to remove the neo cassette. These PP2A-A-alpha-E64G mice were then backcrossed for at least 14 generations to FVB/NCrl mice to establish a colony. Upon arrival at The Jackson Laboratory, these were bred to FVB/NJ (Stock No. 001800) mice for at least one generation.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ppp2r1a
017441 FVB.129S-Ppp2r1atm1.1Wltr/J View Strains carrying other alleles of Ppp2r1a (1 strain)
Phenotype
Phenotype Information
assigned by genotype
Ppp2r1atm3.1Wltr/Ppp2r1atm3.1Wltr
FVB.129-Ppp2r1atm3.1Wltr
- normal phenotype
- no abnormal phenotype detected
- mice are viable (MGI Ref ID J:175748)
This mouse can be used to support research in many areas including:
Cancer Research
Genes Regulating Growth and Proliferation
Tumor Suppressor Genes
Cell Biology Research
DNA Damage Response
Genes Regulating Growth and Proliferation
Signal Transduction
Transcriptional Regulation
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ppp2r1atm3.1Wltr | ||
|---|---|---|---|
| Allele Name | targeted mutation 3.1, Gernot Walter | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | PP2A-A-alpha-E64G; | ||
| Mutation Made By | Gernot Walter, UCSD | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Promoter | Ppp2r1a, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, mouse, laboratory | ||
| Molecular Note | Exon 3 was related with a modified exon 3 in which nucleotide substitutions (GAG to GGC) result in an amino acid substitution of glycine for glutamic acid (E64G). A floxed neo cassette inserted downstream of the modified exon 3 was removed by cre-mediated recombination. [MGI Ref ID J:175748] | ||
Genotyping
Genotyping Information
Genotyping Protocols
** Ppp2r1a*E64G, Pyrosequencing
Ppp2r1atm2.1Wltr, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ruediger R; Ruiz J; Walter G. 2011. Human Cancer-Associated Mutations in the A{alpha} Subunit of Protein Phosphatase 2A Increase Lung Cancer Incidence in A{alpha} Knock-In and Knockout Mice. Mol Cell Biol 31(18):3832-44. [PubMed: 21791616] [MGI Ref ID J:175748]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Heterozygote x Heterozygote (Female x Male) 26-MAR-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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