Description

Strain Information

Former Names B6.129-Reg3gtm1Lvh/J    (Changed: 09-NOV-11 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   06-FEB-13 Species laboratory mouse Generation N10pN1+F2 (15-NOV-12) Generation Definitions   Donating Investigator Lora V. Hooper,   UT Southwestern Medical Center, HHMI

Description
Reg3g (regenerating islet-derived 3 gamma) is a bactericidal C-type lectin that specifically targets Gram-positive bacteria. Its expression is controlled by Myd88 in specialized epithelial cells of the small intestine.

These mice carry a targeted deletion of all six exons of the Reg3g gene. Homozygotes are born at predicted Mendelian ratios, appear healthy, and show no signs of enteropathy by histology. Mice deficient in this protein exhibit increased bacterial adherence to the small intestinal epithelial surface, increased fecal immunoglobulin A (IgA), and increased numbers of intestinal T helper 1 (T_H1) cells. This strain has been useful in studies of bacterial colonization of the small intestine and host intestinal-microbial homeostasis.

Development
A targeting vector was used to replace a 2.6 kb region encoding all six exons of the targeted gene with a FRT-flanked PGK-neomycin resistance cassette. The mutation was created in 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Resultant mice were crossed with a C57BL/6 background FLP recombinase strain to excise the neomycin cassette. This strain was backcrossed to C57BL/6J for ten generations by the donating lab.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Reg3g^tm1.1Lvh/Reg3g^tm1.1Lvh

        B6.129-Reg3g^tm1.1Lvh

• digestive/alimentary phenotype
• *normal* digestive/alimentary phenotype
  • mice exhibit normal intestinal morphology   (MGI Ref ID J:177506)
• • abnormal feces composition
    • increased fecal IgA and albumin   (MGI Ref ID J:177506)
    • however, antibody treatment restores normal fecal IgA levels   (MGI Ref ID J:177506)
  • abnormal gut flora balance
    • mice exhibit a higher mucosal bacterial loads (gram-positive Firmicutes phylum [Lactobacillus, Eubacterium rectale, and segmented filamentous bacteria (SFB) groups]) compared with wild-type mice   (MGI Ref ID J:177506)
    • however, luminal bacterial loads are normal   (MGI Ref ID J:177506)
  • abnormal intestine physiology
    • intestinal permeability (measured by fecal albumin) is increased compared to in wild-type mice   (MGI Ref ID J:177506)
• immune system phenotype
• *normal* immune system phenotype
  • mice exhibit normal numbers of Th17 and regulatory T cells   (MGI Ref ID J:177506)
• • increased IgA level
    • mice exhibit increased IgA+ cells in the small intestinal lamina propria and increased fecal IgA compared with wild-type mice   (MGI Ref ID J:177506)
    • however, antibody treatment restores normal IgA cell numbers   (MGI Ref ID J:177506)
  • increased T-helper 1 cell number
    • in the intestinal lamina propria   (MGI Ref ID J:177506)
    • however, antibody treatment restores normal IFN-gamma-producing Th1 cell numbers   (MGI Ref ID J:177506)
• hematopoietic system phenotype
• increased T-helper 1 cell number
  • in the intestinal lamina propria   (MGI Ref ID J:177506)
  • however, antibody treatment restores normal IFN-gamma-producing Th1 cell numbers   (MGI Ref ID J:177506)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Internal/Organ Research
Gastrointestinal Defects
      colitis

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Reg3gtm1.1Lvh
Allele Name		targeted mutation 1.1, Lora Hooper
Allele Type		Targeted (knock-out)
Strain of Origin		129
Gene Symbol and Name		Reg3g, regenerating islet-derived 3 gamma
Chromosome		6
Gene Common Name(s)		AI449515; PAPIII; Pap3; RegIII (gamma); expressed sequence AI449515;
Molecular Note		A targeting vector was used to replace a 2.6 kb region encoding all six exons of the targeted gene with a FRT-flanked PGK-neomycin resistance cassette. Flp-mediated recombination removed the neo cassette. The absence of transcript expression was confirmed by quantitative RT-PCR on ileum extracts. [MGI Ref ID J:177506] [MGI Ref ID J:177550]

Genotyping

Genotyping Information

Genotyping Protocols

Reg3g^tm1.1Lvhalternate1 MCA,

Separated MCA

Reg3g^tm1.1Lvhalternate1, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hooper L. 2011. Direct Data Submission 2011/11/08 MGI Direct Data Submission :.  [MGI Ref ID J:177550]

Additional References

Reg3g^tm1.1Lvh related

Vaishnava S; Yamamoto M; Severson KM; Ruhn KA; Yu X; Koren O; Ley R; Wakeland EK; Hooper LV. 2011. The antibacterial lectin RegIIIgamma promotes the spatial segregation of microbiota and host in the intestine. Science 334(6053):255-8. [PubMed: 21998396]  [MGI Ref ID J:177506]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & Husbandry	Heterozygotes or homozygotes may be bred.
Mating System	Homozygote x Homozygote         (Female x Male)   06-FEB-13
Diet Information	LabDiet® 5K20

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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