Strain Name:

B6.129-Reg3gtm1.1Lvh/J

Stock Number:

017480

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Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These Reg3g knock-out mice are useful in studies of bacterial colonization of the small intestine and host intestinal-microbial homeostasis.

Description

Strain Information

Former Names B6.129-Reg3gtm1Lvh/J    (Changed: 09-NOV-11 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   06-FEB-13
Specieslaboratory mouse
GenerationN10pN1+F4 (17-DEC-13)
Generation Definitions
 
Donating Investigator Lora V. Hooper,   UT Southwestern Medical Center, HHMI

Description
Reg3g (regenerating islet-derived 3 gamma) is a bactericidal C-type lectin that specifically targets Gram-positive bacteria. Its expression is controlled by Myd88 in specialized epithelial cells of the small intestine.

These mice carry a targeted deletion of all six exons of the Reg3g gene. Homozygotes are born at predicted Mendelian ratios, appear healthy, and show no signs of enteropathy by histology. Mice deficient in this protein exhibit increased bacterial adherence to the small intestinal epithelial surface, increased fecal immunoglobulin A (IgA), and increased numbers of intestinal T helper 1 (TH1) cells. This strain has been useful in studies of bacterial colonization of the small intestine and host intestinal-microbial homeostasis.

Development
A targeting vector was used to replace a 2.6 kb region encoding all six exons of the targeted gene with a FRT-flanked PGK-neomycin resistance cassette. The mutation was created in 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Resultant mice were crossed with a C57BL/6 background FLP recombinase strain to excise the neomycin cassette. This strain was backcrossed to C57BL/6J for ten generations by the donating lab.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Reg3gtm1.1Lvh/Reg3gtm1.1Lvh

        B6.129-Reg3gtm1.1Lvh
  • digestive/alimentary phenotype
  • *normal* digestive/alimentary phenotype
    • mice exhibit normal intestinal morphology   (MGI Ref ID J:177506)
    • abnormal feces composition
      • increased fecal IgA and albumin   (MGI Ref ID J:177506)
      • however, antibody treatment restores normal fecal IgA levels   (MGI Ref ID J:177506)
    • abnormal gut flora balance
      • mice exhibit a higher mucosal bacterial loads (gram-positive Firmicutes phylum [Lactobacillus, Eubacterium rectale, and segmented filamentous bacteria (SFB) groups]) compared with wild-type mice   (MGI Ref ID J:177506)
      • however, luminal bacterial loads are normal   (MGI Ref ID J:177506)
    • abnormal intestine physiology
      • intestinal permeability (measured by fecal albumin) is increased compared to in wild-type mice   (MGI Ref ID J:177506)
  • immune system phenotype
  • *normal* immune system phenotype
    • mice exhibit normal numbers of Th17 and regulatory T cells   (MGI Ref ID J:177506)
    • increased IgA level
      • mice exhibit increased IgA+ cells in the small intestinal lamina propria and increased fecal IgA compared with wild-type mice   (MGI Ref ID J:177506)
      • however, antibody treatment restores normal IgA cell numbers   (MGI Ref ID J:177506)
    • increased T-helper 1 cell number
      • in the intestinal lamina propria   (MGI Ref ID J:177506)
      • however, antibody treatment restores normal IFN-gamma-producing Th1 cell numbers   (MGI Ref ID J:177506)
  • hematopoietic system phenotype
  • increased IgA level
    • mice exhibit increased IgA+ cells in the small intestinal lamina propria and increased fecal IgA compared with wild-type mice   (MGI Ref ID J:177506)
    • however, antibody treatment restores normal IgA cell numbers   (MGI Ref ID J:177506)
  • increased T-helper 1 cell number
    • in the intestinal lamina propria   (MGI Ref ID J:177506)
    • however, antibody treatment restores normal IFN-gamma-producing Th1 cell numbers   (MGI Ref ID J:177506)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Intracellular Signaling Molecules

Internal/Organ Research
Gastrointestinal Defects
      colitis

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Reg3gtm1.1Lvh
Allele Name targeted mutation 1.1, Lora Hooper
Allele Type Targeted (Null/Knockout)
Strain of Origin129
Gene Symbol and Name Reg3g, regenerating islet-derived 3 gamma
Chromosome 6
Gene Common Name(s) AI449515; LPPM429; PAP IB; PAP-1B; PAP1B; PAPIB; PAPIII; Pap3; REG III; REG-III; RegIII (gamma); UNQ429; expressed sequence AI449515;
Molecular Note A targeting vector was used to replace a 2.6 kb region encoding all six exons of the targeted gene with a FRT-flanked PGK-neomycin resistance cassette. Flp-mediated recombination removed the neo cassette. The absence of transcript expression was confirmed by quantitative RT-PCR on ileum extracts. [MGI Ref ID J:177506] [MGI Ref ID J:177550]

Genotyping

Genotyping Information

Genotyping Protocols

Reg3gtm1.1Lvhalternate1 MCA,

Separated MCA


Reg3gtm1.1Lvhalternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Hooper L. 2011. Direct Data Submission 2011/11/08 MGI Direct Data Submission :.  [MGI Ref ID J:177550]

Additional References

Reg3gtm1.1Lvh related

Vaishnava S; Yamamoto M; Severson KM; Ruhn KA; Yu X; Koren O; Ley R; Wakeland EK; Hooper LV. 2011. The antibacterial lectin RegIIIgamma promotes the spatial segregation of microbiota and host in the intestine. Science 334(6053):255-8. [PubMed: 21998396]  [MGI Ref ID J:177506]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryHeterozygotes or homozygotes may be bred.
Mating SystemHomozygote x Homozygote         (Female x Male)   06-FEB-13
Diet Information LabDiet® 5K20

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Reg3gtm1.1Lvh  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Reg3gtm1.1Lvh x Homozygous for Reg3gtm1.1Lvh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Reg3gtm1.1Lvh  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Reg3gtm1.1Lvh x Homozygous for Reg3gtm1.1Lvh  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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