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| These iZ/WAP mice express a Cre recombinase-inducible wheat germ agglutinin in cre-expressing neurons, and have applications as a trans-synaptic tracer or marker. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names C57BL/6-Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj/J (Changed: 20-MAR-12 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 08-MAY-12 Mating System Hemizygote x Noncarrier (Female x Male) 08-MAY-12 Species laboratory mouse Generation F?pN1+
Generation DefinitionsDonating Investigator Martin G Myers, University of Michigan Medical School Description
These transgenic mice express a Cre recombinase-inducible wheat germ agglutinin lectin (WGA), under the direction of the CMV, cytomegalovirus, promoter. This strain serves as a reporter strain, with successful Cre excision being indicated by WGA expression in cre-expressing neurons. WGA acts as a trans-synaptic tracer, making it possible to identify the synaptic contacts of cre-expressing neurons. Alkaline phosphatase activity is not detected in the CNS. Mice that are hemizygous for the transgene are viable, normal in size and do not display any gross physical or behavioral abnormalities. Transgene expression in the iZ/WAP strain is more widespread throughout the CNS when compared to the similar strain, B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J (Stock No. 006465).Development
A transgenic construct containing a floxed β-geo (lacZ and NEO) cassette, wheat germ agglutinin (WGA) coding sequence, IRES sequence, and human alkaline phosphatase (placental) sequence under the control of the CMV, cytomegalovirus, promoter was introduced into 129 derived R1 embryonic stem (ES) cells. ES cells containing the construct were injected into recipient blastocysts to generate chimeric mice. Resulting male chimeric animals were bred with C57BL/6 female mice. A founder line with transgene expression throughout the CNS was selected. The mice were crossed to C57BL/6 for 2 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of ALPP
012377 B6.129S-Cyp19a1tm1.1Shah/J 012588 B6.Cg-Tg(TH-ALPP)1Erav/J 008226 B6.FVB-Tg(CAG-EGFP,-ALPP)2.6Ggc/J 007066 B6.SJL-Tg(Crx-GFP,-ALPP)1Clc/J 016555 B6;SJL-Tg(Nqo1-ALPP)1Jaj/J 008200 FVB/N-Tg(CAG-EGFP,-ALPP)2.6Ggc/J 006882 STOCK Tg(CAG-Bgeo,-AML1/ETO,-ALPP)1Lbe/J 006613 STOCK Tg(CAG-Bgeo,-Tle1,-ALPP)1Lbe/J 003919 STOCK Tg(CAG-Bgeo/ALPP)1Lbe/J 005105 STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J 014600 STOCK Tg(I12b-cre/ERT2,-ALPP)37Fsh/J View Strains carrying other alleles of ALPP (11 strains)
006465 B6;CBA-Tg(CAG-lacZ-WGA)330Bbm/J
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Bgeo relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
Research Tools
Cre-lox System
loxP-flanked Sequences
Genetics Research
Tissue/Cell Markers: Cre-lox System
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Martin G Myers, Jr | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | iZ/WAP; | ||
| Mutation Made By | Martin Myers, University of Michigan Medical School | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lacZ expression is seen in all cells prior to Cre recombination. After cre excision of the floxed- lacZ-neo cassette, wheat germ agglutinin (WGA) and human alkaline phosphatase (placental) are expressed in cre-expressing tissues. | ||
| Expressed Gene | ALPP, alkaline phosphatase, placental, human | ||
| Expressed Gene | WGA, Wheat Germ Agglutin, truncated, | ||
| Expressed Gene | Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli | ||
| Promoter | CMV, cytomegalovirus, human | ||
| Molecular Note | The transgene contains a floxed beta-geo (lacZ and NEO) cassette, wheat germ agglutinin (WGA) coding sequence, IRES sequence, and human alkaline phosphatase (placental) sequence under the control of the CMV, cytomegalovirus, promoter. Line 1 was generated. [MGI Ref ID J:163528] | ||
| Gene Symbol and Name | Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj, transgene insertion 1, Martin G Myers, Jr | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj-alternate1, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Louis GW; Leinninger GM; Rhodes CJ; Myers MG Jr. 2010. Direct innervation and modulation of orexin neurons by lateral hypothalamic LepRb neurons. J Neurosci 30(34):11278-87. [PubMed: 20739548] [MGI Ref ID J:163528]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice can be bred as hemizygotes. Viability and fertility of homozygotes have not been confirmed. Mating System Noncarrier x Hemizygote (Female x Male) 08-MAY-12 Hemizygote x Noncarrier (Female x Male) 08-MAY-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(CMV-Bgeo,-WGA,-ALPP)1Mgmj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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