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| These transgenic mice express the human insulin gene and are useful in studies of diabetes. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Dr. Richard Mulligan, Harvard Medical School Description
This strain of mice, first published in 1986 on a mixed C57BL/6-C3H genetic background, successfully demonstrated the transgenic expression of human insulin in the pancreas. Human pro-insulin is enzymatically cleaved into insulin and C-peptide in pancreatic islets. In these mice, no human C-peptide was detected in the sera of fasting transgenic mice, but within 10 minutes of i.p. administration of glucose, human C-peptide appeared in the serum. Peak levels were obtained within ~20 minutes. By 45 minutes post-glucose, human C-peptide levels fell to values approaching the pre-stimulation or basal level.Injected with human insulin, these mice demonstrated an acquired tolerance to human insulin and pro-insulin. These animals showed no antibody response. This model presented a novel alternative to the induction of tolerance in newborn mice using antigenic dosing.
The current line offered here has been backcrossed to BALB/cJ and is on a homozygous H2d background.
Development
A 12.5 kb EcoRI fragment containing the human insulin gene was microinjected into C57BL/6 x C3H F2 embryos by Howard Goodman's lab at Harvard University. Originally published in 1986, H2k progeny were subsequently selected by Judith Kapp's group at The Jewish Hospital of St. Louis at Washington University Medical School and backcrossed to BALB/cJ (H2d) for at least 10 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of INS
006411 B6.129-Gasttm1(INS)Ez/J 005115 NOD.FVB-Tg(INS-MT2A,Tyr)1Pne/PneJ 005113 NOD.FVB-Tg(INS-SOD2)3Pne/PneJ 010632 NOD/ShiLt-Tg(INS-NP)6171Mvh/J View Strains carrying other alleles of INS (4 strains)
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Diabetes Mellitus, Insulin-Dependent, 2 (INS)
Diabetes Mellitus, Permanent Neonatal; PNDM (INS)
Insulin; INS (INS)
Maturity-Onset Diabetes of the Young, Type 10; MODY10 (INS)
This mouse can be used to support research in many areas including:
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(INS)1Gdmn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Howard M Goodman | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Howard Goodman, Harvard Medical School - Retired | ||
| Strain of Origin | (C57BL/6 x C3H)F2 | ||
| Expressed Gene | INS, insulin, human | ||
| Promoter | INS, insulin, human | ||
| Molecular Note | The transgene contains a 12.5 kb EcoRI fragment containing the human insulin gene. Line 1 was generated. [MGI Ref ID J:179197] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Human INS cDNA, QPCR
Tg(INS)1Gdmn, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Selden RF; Skoskiewicz MJ; Howie KB; Russell PS; Goodman HM. 1986. Regulation of human insulin gene expression in transgenic mice. Nature 321(6069):525-8. [PubMed: 3520336] [MGI Ref ID J:179197]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Homozygotes or heterozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000651 BALB/cJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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