Strain Name:

B6;129S-Tet2tm1.1Iaai/J

Stock Number:

017573

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Availability:

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The Tet2f mutation has loxP sites flanking exon 3 of the tet oncogene family member 2 gene (Tet2). These floxed mice may be used for conditional knockout of Tet2 for studying hematopoietic stem cell self-renewal and myeloid transformation.

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHomozygote x Homozygote         (Female x Male)   20-JUN-13
Specieslaboratory mouse
Generation?+N1F3 (10-DEC-13)
Generation Definitions
 
Donating Investigator Iannis Aifantis,   NYU School of Medicine

Description
Like TET1 and TET3, TET2 modifies DNA by hydroxylating 5 methylcytosine. A significant percentage of patients with myeloid malignancies exhibit mutations in TET2, of these, 41.5% of mutations occur in the first coding exon (mouse exon 3). These Tet2f/f mice possess loxP sites flanking exon 3. Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. This mutant mouse strain may be useful in generating conditional mutations to study hematopoietic stem cell self-renewal and myeloid transformation.

For example, when crossed to a strain expressing interferon-inducible Cre recombinase (see Stock No. 003556), mice exhibit increased hematopoietic stem cell self-renewal.

Development
A targeting vector was designed to place an FRT- and loxP-flanked neomycin resistance cassette upstream of exon 3 and a loxP site downstream of exon 3. The construct was electroporated into BAC-BA1(129S/SvEV x C57BL/6)-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Strong chimeras were identified and crossed to C57BL/6 females to verify germline transmission. The F1 generation was PCR screened and crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (JR#003946) to remove the FRT neo cassette.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tet2
023359   B6(Cg)-Tet2tm1.2Rao/J
View Strains carrying other alleles of Tet2     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Myelodysplastic Syndrome; MDS   (TET2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Tet2tm1.1Iaai/Tet2tm1.1Iaai Tg(EIIa-cre)C5379Lmgd/0

        involves: 129S/SvEv * C57BL/6 * FVB/N   (conditional)
  • normal phenotype
  • no abnormal phenotype detected
    • mice are born with at the expected Mendelian ratio with normal growth and organ development   (MGI Ref ID J:174053)

Tet2tm1.1Iaai/Tet2tm1.1Iaai Tg(Mx1-cre)1Cgn/0

        involves: 129S/SvEv * C57BL/6 * CBA   (conditional)
  • hematopoietic system phenotype
  • abnormal hematopoietic stem cell physiology
    • in transplantation experiments, pIpC-treated hematopoietic stem cells exhibit increased self-renewal compared with control cells   (MGI Ref ID J:174053)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cancer Research
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tet2tm1.1Iaai
Allele Name targeted mutation 1.1, Iannis Aifantis
Allele Type Targeted (Floxed/Frt)
Common Name(s) Tet2f;
Mutation Made By Iannis Aifantis,   NYU School of Medicine
Strain of OriginC57BL/6 x 129S/SvEv
Gene Symbol and Name Tet2, tet methylcytosine dioxygenase 2
Chromosome 3
Gene Common Name(s) Ayu17-449; E130014J05Rik; KIAA1546; MDS; RGD1311625; RIKEN cDNA E130014J05 gene; mKIAA1546;
General Note ES cell = BAC-BA1 (C57BL/6 x 129S/SvEv)
Molecular Note An FRT- and loxP-flanked neo cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the neo cassette and left exon 3 floxed. [MGI Ref ID J:174053]

Genotyping

Genotyping Information

Genotyping Protocols

Tet2tm1.1Iaai, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Moran-Crusio K; Reavie L; Shih A; Abdel-Wahab O; Ndiaye-Lobry D; Lobry C; Figueroa ME; Vasanthakumar A; Patel J; Zhao X; Perna F; Pandey S; Madzo J; Song C; Dai Q; He C; Ibrahim S; Beran M; Zavadil J; Nimer SD; Melnick A; Godley LA; Aifantis I; Levine RL. 2011. Tet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation. Cancer Cell 20(1):11-24. [PubMed: 21723200]  [MGI Ref ID J:174053]

Additional References

Tet2tm1.1Iaai related

Deplus R; Delatte B; Schwinn MK; Defrance M; Mendez J; Murphy N; Dawson MA; Volkmar M; Putmans P; Calonne E; Shih AH; Levine RL; Bernard O; Mercher T; Solary E; Urh M; Daniels DL; Fuks F. 2013. TET2 and TET3 regulate GlcNAcylation and H3K4 methylation through OGT and SET1/COMPASS. EMBO J 32(5):645-55. [PubMed: 23353889]  [MGI Ref ID J:194478]

Doege CA; Inoue K; Yamashita T; Rhee DB; Travis S; Fujita R; Guarnieri P; Bhagat G; Vanti WB; Shih A; Levine RL; Nik S; Chen EI; Abeliovich A. 2012. Early-stage epigenetic modification during somatic cell reprogramming by Parp1 and Tet2. Nature 488(7413):652-5. [PubMed: 22902501]  [MGI Ref ID J:187148]

Kallin EM; Rodriguez-Ubreva J; Christensen J; Cimmino L; Aifantis I; Helin K; Ballestar E; Graf T. 2012. Tet2 facilitates the derepression of myeloid target genes during CEBPalpha-induced transdifferentiation of pre-B cells. Mol Cell 48(2):266-76. [PubMed: 22981865]  [MGI Ref ID J:191279]

Lobry C; Ntziachristos P; Ndiaye-Lobry D; Oh P; Cimmino L; Zhu N; Araldi E; Hu W; Freund J; Abdel-Wahab O; Ibrahim S; Skokos D; Armstrong SA; Levine RL; Park CY; Aifantis I. 2013. Notch pathway activation targets AML-initiating cell homeostasis and differentiation. J Exp Med 210(2):301-19. [PubMed: 23359070]  [MGI Ref ID J:196407]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   20-JUN-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Tet2tm1.1Iaai  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Tet2tm1.1Iaai x Homozygous for Tet2tm1.1Iaai  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Tet2tm1.1Iaai  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Tet2tm1.1Iaai x Homozygous for Tet2tm1.1Iaai  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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