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| YFP and Cre are expressed from the basophil-specific Mcpt8 (mast cell protease 8; also known as Basoph8) promoter in this targeted mutation strain. This strain is useful for labeling, sorting and deleting basophil cell populations. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names B6.129-Mcpt8tm1(cre)Lksy/J (Changed: 08-NOV-11 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 21-FEB-13 Species laboratory mouse Generation N10+N2F1 (11-JUN-13)
Generation DefinitionsDonating Investigator Richard M. Locksley, Univ of California San Francisco-HHMI Description
This targeted mutant strain expresses both yellow fluorescent protein (YFP) and Cre recombinase from the Mcpt8 (mast cell protease 8; also known as Basoph8) promoter. The YFP reporter labels MCPT8-expressing cells, enabling lineage marking of basophils as well as cell sorting by flow cytometry. Cre excision of loxP-flanked sequences is basophil-specific when these mice are bred with a floxed animal. Expression of the endogenous Mcpt8 gene is blocked, but basophil functions are not impaired.When crossed with a strain expressing a floxed stop-diptheria toxin α from the Gt(ROSA26)Sor promoter (e.g. Stock No. 009669), selective deletion of basophils can be achieved.
Development
Genomic sequence of the rabbit β-globin gene, enhanced yellow fluorescent protein (YFP), IRES, humanized Cre (mammalian-optimized), bovine growth hormone poly (A), and a loxP-flanked neomycin cassette were introduced to the mouse Mcpt8 (Basoph8) gene immediately after the 5' promoter and UTR. 129-derived embryonic stem cells encoding Prm-Cre were used to create the mutation and excise the neomycin resistance cassette. This strain was backcrossed to C57BL/6 for 10 generations by the donating lab.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Mcpt8tm1(cre)Lky allele
017582 C.129S4(B6)-Mcpt8tm1(cre)Lky/J View Strains carrying Mcpt8tm1(cre)Lky (1 strain)
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Mcpt8tm1(cre)Lky/?
either: (involves: 129S4/SvJae * BALB/c) or (involves: 129S4/SvJae * C57BL/6)
- no phenotypic analysis
- *normal* no phenotypic analysis
- no details are provided; mice are used in a variety of crosses (MGI Ref ID J:172122)
This mouse can be used to support research in many areas including:
YFP relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Fluorescent Proteins
Immunology and Inflammation Research
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Mcpt8tm1(cre)Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard M Locksley | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Basoph8; Mcpt8tm1(cre)Lksy; | ||
| Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og | ||
| Site of Expression | YFP is expressed in basophils. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Driver Note | Mcpt8 | ||
| Molecular Note | A 4.8 kb reporter cassette containing YFP, IRES site, humanized cre recombinase, bGH polyA, and a loxP-flanked neo selection marker was inserted at the Mcpt8 start site via homologous recombination in ES cells contain PrmCre (PC3 cells with Tg(Prm-cre)70Og). Upon crossing male chimeras to wild-type females, the loxP-flanked neo was deleted from the male germline by cre recombination, producing the final knock-in allele. [MGI Ref ID J:172122] | ||
| Gene Symbol and Name | Mcpt8, mast cell protease 8 | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | BB139338; MMCP-8; RMCP8; expressed sequence BB139338; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Mcpt8tm1(cre)Lksy, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Sullivan BM; Liang HE; Bando JK; Wu D; Cheng LE; McKerrow JK; Allen CD; Locksley RM. 2011. Genetic analysis of basophil function in vivo. Nat Immunol 12(6):527-35. [PubMed: 21552267] [MGI Ref ID J:172122]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry Homozygotes or heterozygotes may be bred. Mating System Homozygote x Homozygote (Female x Male) 21-FEB-13 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Mcpt8tm1(cre)Lky
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Mcpt8tm1(cre)Lky x Homozygous for Mcpt8tm1(cre)Lky Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Mcpt8tm1(cre)Lky
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Mcpt8tm1(cre)Lky x Homozygous for Mcpt8tm1(cre)Lky Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
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|
|
Standard Supply
Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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