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| The "interferon-gamma reporter with endogenous polyA transcript" (GREAT) allele has an IRES-eYFP reporter cassette inserted between the translational stop codon and 3' UTR/polyA tail of the interferon gamma (Ifng) gene. Thus, the bicistronic IFNγ-IRES-eYFP mRNA is under control of the endogenous IFNγ promoter/enhancer regions with proper regulation defined by the endogenous 3' UTR and polyA tail. These GREAT mice are a fluorescent reporter strain for studying innate immunity/adaptive immunity against viral and intracellular bacterial infections, for studying anti-tumor biology, as well for studying aberrant IFN-γ expression associated with autoinflammatory/autoimmune diseases. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Former Names C.129S4(B6)-Ifngtm3(EYFP)Lky/J (Changed: 07-MAY-12 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 14-DEC-12 Species laboratory mouse Donating Investigator Richard M. Locksley, Univ of California San Francisco-HHMI Description
The "interferon-gamma reporter with endogenous polyA transcript" (GREAT) allele has an IRES-eYFP reporter cassette inserted between the translational stop codon and 3' UTR/polyA tail of the interferon gamma (Ifng) gene. Thus, the bicistronic IFNγ-IRES-eYFP mRNA is under control of the endogenous IFNγ promoter/enhancer regions with proper regulation defined by the endogenous 3' UTR and polyA tail. Expression of eYFP is observed by FACS/immunofluorescence microscopy in Ifng-expressing cell types; including innate immune responding cells (natural killer [NK] cells, natural killer T [NKT] cells) and cytotoxic T lymphocyte (CTL) effector cells of the adaptive immune response (CD4+ and CD8+T cells).Of note, the donating investigator reports that these GREAT mice are a more useful tool than their IFNγ-IRES-YFP-BGHpolyA knockin (YETI) mice. Because the YETI allele has a bovine growth hormone polyA tail (rather than the endogenous IFN-γ polyA tail), homozygous YETI mice exhibit aberrant bicistronic IFNγ-IRES-YFP mRNA regulation that results in high IFN-γ blood levels and premature death. In contrast, homozygous GREAT mice are viable, fertile and normal in size, with proper transcript regulation defined by the endogenous IFN-γ 3' UTR/polyA tail.
Development
A targeting vector was designed with a loxP-flanked neomycin resistance cassette, an internal ribosome entry site (IRES), and an enhanced yellow fluorescent protein (eYFP) sequence. This vector was inserted downstream of the endogenous translational stop codon and upstream of the 3' untranslated region (UTR) and polyA tail of the interferon gamma (Ifng) gene via electroporation into 129S4/SvJae-derived PrmCre embryonic stem (ES) cells. These PrmCre (or PC3) ES cells contain the Tg(Prm-cre)70Og transgene with the protamine promoter directing cre expression to the male germline. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred to C57BL/6 females. The male offspring from this initial breeding lack the floxed neo cassette in their germline, and were bred to female C57BL/6 mice. Offspring with the IRES-eYFP cassette and without the Pmc-Cre transgene were identified to establish the colony of "interferon-gamma reporter with endogenous polyA transcript" (GREAT) mice. These GREAT mice were subsequently backcrossed to BALB/cJ mice for at least ten generations. Upon arrival at The Jackson Laboratory Repository, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation.
Control Information
| Control | ||
|---|---|---|
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
017581 B6.129S4-Ifngtm3.1Lky/J
002287 B6.129S7-Ifngtm1Ts/J 009344 B6.Cg-Tg(tetO-Ifng)184Pop/J 002286 C.129S7(B6)-Ifngtm1Ts/J 008228 C3.129S7(B6)-Ifngtm1Ts/J 002575 NOD.129S7(B6)-Ifngtm1Ts/DvsJ
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Aplastic Anemia (IFNG)
Hepatitis C Virus, Susceptibility to (IFNG)
Human Immunodeficiency Virus Type 1, Susceptibility to (IFNG)
Mycobacterium Tuberculosis, Susceptibility to (IFNG)
Tuberous Sclerosis 2; TSC2 (IFNG)
assigned by genotype
Ifngtm3.1Lky/Ifngtm3.1Lky
C.129S4-Ifngtm3.1Lky
- normal phenotype
- no abnormal phenotype detected
- mice are viable, fertile and normal in size (MGI Ref ID J:183078)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ifngtm3.1Lky/Ifngtm3.1Lky
B6.129S4-Ifngtm3.1Lky
- normal phenotype
- no abnormal phenotype detected
- mice are viable, fertile and normal in size (MGI Ref ID J:183078)
This mouse can be used to support research in many areas including:
Cancer Research
Growth Factors/Receptors/Cytokines
Other
Tumor Resistance
Tumor Suppressor Genes
Cell Biology Research
Signal Transduction
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
genes regulating susceptibility to infectious disease and endotoxin
Growth Factors/Receptors/Cytokines
Immunodeficiency
Intracellular Signaling Molecules
Research Tools
Cancer Research
B, T, and NK cell deficiency, xenograft/transplant host
T cell deficiency
anti-tumor activity
tumor immunology
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Immunology and Inflammation Research
NK Cell Deficiency
genes regulating susceptibility to infectious disease and endotoxin
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ifngtm3.1Lky | ||
|---|---|---|---|
| Allele Name | targeted mutation 3.1, Richard M Locksley | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | GREAT; Ifngtm3(EYFP)Lky; | ||
| Mutation Made By | Richard Locksley, Univ of California San Francisco-HHMI | ||
| Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og | ||
| Site of Expression | Cells activated to express Ifng will express EYFP. | ||
| Gene Symbol and Name | Ifng, interferon gamma | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | IFG; IFI; IFN-gamma; IFNG2; Ifg; | ||
| General Note | Smart (Surface marker for transcription)-17A | ||
| Molecular Note | A targeting vector was designed with a loxP-flanked neomycin resistance cassette, an internal ribosome entry site (IRES), and an enhanced yellow fluorescent protein (eYFP) sequence. This vector was inserted downstream of the endogenous translational stopcodon and upstream of the 3' untranslated region (UTR). Cre-mediated recombination removed the neo cassette. [MGI Ref ID J:147784] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Il4tm1Lkyalternate1, Separated PCR
Il4tm1Lky, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Reinhardt RL; Liang HE; Locksley RM. 2009. Cytokine-secreting follicular T cells shape the antibody repertoire. Nat Immunol 10(4):385-93. [PubMed: 19252490] [MGI Ref ID J:147784]
Additional References
Ifngtm3.1Lky relatedLocksley RM. 2012. Direct Data Submission 2012/05/07 MGI Direct Data Submission :. [MGI Ref ID J:183078]
Price AE; Reinhardt RL; Liang HE; Locksley RM. 2012. Marking and quantifying IL-17A-producing cells in vivo. PLoS One 7(6):e39750. [PubMed: 22768117] [MGI Ref ID J:187909]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Mating System Heterozygote x Heterozygote (Female x Male) 14-DEC-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Ifngtm3.1Lky $232.00 Female or Male Homozygous for Ifngtm3.1Lky
Price per Pair (US dollars $) Pair Genotype $464.00 Heterozygous for Ifngtm3.1Lky x Heterozygous for Ifngtm3.1Lky Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Ifngtm3.1Lky $301.60 Female or Male Homozygous for Ifngtm3.1Lky
Price per Pair (US dollars $) Pair Genotype $603.20 Heterozygous for Ifngtm3.1Lky x Heterozygous for Ifngtm3.1Lky Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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