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| These OVE1739A mice harbor a mutation created by random insertion of co-injected transgenes pT-Tybs-3'E and Prm-SB10. Using inverse PCR analysis, the integration site of the co-injected transgenes is near the eyes absent 4 homolog [Drosophila] locus (Eya4) on mouse chromosome 10. These OVE1739A mice may be useful for studying cleft palate, as well as middle ear morphology, otitis media, and incomplete fusion of palatal bones during skull development. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
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- References
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Description
Strain Information
Type Transposon Induced Mutation; Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F?pN1+F1 (11-MAR-13)
Generation DefinitionsDonating Investigator Paul A Overbeek, Baylor College of Medicine Description
These OVE1739A mice harbor a mutation created by random insertion of co-injected transgenes pT-Tybs-3'E and Prm-SB10. Using inverse PCR analysis, the integration site of the co-injected transgenes is near the eyes absent 4 homolog [Drosophila] locus (Eya4) on mouse chromosome 10. The integration site is reported to be chr10 A3(+):22,797,990 bp [NCB137/mm9]: this is ~25 kbp away from Eya4 (22,823,974-23,069,701 [NCB137/mm9]). The donating investigator reports no Eya4 expression (via RT-PCR) from the mutatant allele. Heterozygous and homozygous mice exhibit grey coat color.The donating investigator reports the phenotype of heterozygous OVE1739A mice as:
Heterozygous mice are viable and fertile, but heterozygous females are not reliable mothers and heterozygous fathers may need to be removed from cages with newborns since they trample the pups. Heterozygous sibling males that are weaned together often start to fight at 6-10 weeks of age. Heterozygotes are typically (but not always) hyperactive and show circling behavior, especially when they are moved to a new cage during routine husbandry.The donating investigator reports the phenotype of homozygous OVE1739A mice as:
All homozygotes mice die at birth with cleft palate. Although mice homozygous for other published Eya4 mutations exhibit abnormal middle ear morphology, otitis media, incomplete fusion of palatal bones in skulls of newborn mice, and postnatal lethality, the OVE1739A mice have not yet been examined for similar phenotypes to date (January 2012). OVE1739A mice carry at least one copy of the Prm-SB10 transgene. Although breeding OVE1739A males results in SB transposase expression and possible transposition in the offspring, the donating investigator reports no evidence of re-mobilization of the transposon.Development
A Sleeping Beauty (SB) transposon transgenic approach using co-injection of the transposon vector pT-Tybs-3'E and the SB-expressing transgene Prm-SB10.The pT-Tybs-3'E transposon vector used here has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), mouse tyrosinase enhancer sequence (3.65 kbp), Tyro minigene (TyBS; 4.1 kbp), and right IR/DR (280 bp). The mouse tyrosinase enhancer sequence is from the region positioned ~14 kbp upstream from exon 1 in the mouse genome. The tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyrs-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). The IR/DR sequences are outward-facing (pointed away from the Tyro sequences). The Prm-SB10 transgene used here (prm-SB10-rabbit β-globin plasmid #1611) has the mouse protamine 1 promoter (652 bp), a 25 bp linker, SB10 gene (1023 bp), and rabbit β-globin splice/polyA sequence (1170 bp). Both transgenes were microinjected into one-cell stage FVB/N embryos. Expression of the tyrosinase minigene results in melanin synthesis, and founder mice (F0) were identified by inspection for pigmentation. F0 mice were assigned an OVE number and then bred with FVB/N mice. One pigmented offspring (F1) had grey coat color and was designated OVE1739A. Males harboring both co-injected transgenes have the ability to mobilize transposons in their germline; and mobilization of the IR/DR site-flanked transposon can result in transposon integration at new sites in the genome (as well as genomic rearrangements including deletions, inversions and translocations). Breeding OVE1739A males with FVB/N females resulted in SB transposase expression and transposition in the offspring. These mice were next bred together to assess phenotype. Homozygous mice die at birth with cleft palate. Inverse PCR analysis identified that the integration site of the co-injected transgenes is near the eyes absent 4 homolog [Drosophila] locus (Eya4) on mouse chromosome 10. The integration site is reported to be chr10 A3(+):22,797,990 bp [NCB137/mm9]: this is ~25 kbp away from Eya4 (22,823,974-23,069,701 [NCB137/mm9]). Although the OVE1739A mice still carry at least one copy of the SB10 transgene, the donating investigator reports no evidence of re-mobilization of the transposon. These mice are not expected to have genetic background contributions from C57BL/6. Transgenic males were sent to The Jackson Laboratory Repository Facebase collection. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: models
View Facebase: models (58 strains)
Additional Web Information
Use of MICE by companies or for-profit entities requires a license prior to shipping.
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Cardiomyopathy, Dilated, 1J; CMD1J (EYA4)
Deafness, Autosomal Dominant 10; DFNA10 (EYA4)
assigned by genotype
Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve
involves: C57BL/6 * FVBThis mouse can be used to support research in many areas including:
Developmental Biology Research
Perinatal Lethality
Homozygous
Postnatal Lethality
Homozygous
Neurobiology Research
Hearing Defects
Sensorineural Research
Hearing Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve | ||
|---|---|---|---|
| Allele Name | transgenic transposon concatemer 1739A, Paul A Overbeek | ||
| Allele Type | Transgenic (random, gene disruption) | ||
| Common Name(s) | OVE#1739A; OVE1739A; | ||
| Mutation Made By | Paul Overbeek, Baylor College of Medicine | ||
| Strain of Origin | FVB/N | ||
| Gene Symbol and Name | Eya4, eyes absent 4 homolog (Drosophila) | ||
| Chromosome | 10 | ||
| Gene Common Name(s) | B130023L16Rik; CMD1J; DFNA10; RIKEN cDNA B130023L16 gene; | ||
| Molecular Note |
A Sleeping Beauty (SB) transposon transgenic approach using co-injection of the transposon vector pT-Tybs-3'E and the SB-expressing transgene Prm-SB10. The pT-Tybs-3'E transposon vector used here has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), mouse tyrosinase enhancer sequence (3.65 kbp), Tyro minigene (TyBS; 4.1 kbp), and right IR/DR (280 bp). The mouse tyrosinase enhancer sequence is from the region positioned ~14 kbp upstream from exon 1 in the mouse genome. The tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyrs-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). The donating investigator did not report whether the IR/DR sequences are outward-facing (pointed away from the Tyro sequences) or inward-facing (pointed toward the Tyro sequences). The Prm-SB10 transgene used here (prm-SB10-rabbit β-globin plasmid #1611) has the mouse protamine 1 promoter (652 bp), a 25 bp linker, SB10 gene (1023 bp), and rabbit β-globin splice/polyA sequence (1170 bp). Inverse PCR analysis identified that the integration site of the co-injected transgenes is on chr10 A3(+):22,797,990 bp [NCB137/mm9]: ~25 kbp away from | ||
Genotyping
Genotyping Information
Genotyping Protocols
PGK2-SB10 (OVE1780) ,Separated MCA
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve relatedOverbeek PA. 2011. Direct Data Submission for Overbeek Lentiviral Transgenic Lines MGI Direct Data Submission :. [MGI Ref ID J:175597]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to FVB/NJ inbred mice (Stock No. 001800). All homozygous mice die at birth with complications from cleft palate. The donating investigator also reports heterozygous females are not reliable mothers and heterozygous fathers may need to be removed from cages with newborns since they trample the pups. Heterozygous sibling males that are weaned together often start to fight at 6-10 weeks of age. Heterozygotes are typically (but not always) hyperactive and show circling behavior, especially when they are moved to a new cage during routine husbandry.
Diet Information LabDiet® 5K20
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve x Wild-type for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve $296.00 Wild-type for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve x Heterozygous for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve x Wild-type for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve $384.80 Wild-type for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve x Heterozygous for Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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