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| These OVE2161B-CA1C-2 mice harbor a loss-of-function mutation created by random insertion of the pT2-BART3 transposon transgene into the serologically defined colon cancer antigen 8 gene (Sdccag8) on chromosome 1. These mice may be useful for studying cleft palate, preaxial polydactyly, and polycystic kidney disease. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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- Terms of use
Description
Strain Information
Type Transposition; Additional information on Mice with Chromosomal Aberrations. Type Coisogenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Wild-type x Heterozygote (Female x Male) 02-MAR-12 Mating System Heterozygote x Wild-type (Female x Male) 02-MAR-12 Species laboratory mouse Generation ?+F (26-JUL-12)
Generation DefinitionsDonating Investigator Paul A Overbeek, Baylor College of Medicine Description
These OVE2161B-CA1C-2 mice harbor a mutation created by random insertion of the pT2-BART3 transposon transgene. Using inverse PCR analysis, the integration site was identified between exons 12-13 of the serologically defined colon cancer antigen 8 gene (Sdccag8) on chromosome 1. The transgene is linked to the (+) strand of DNA at position 178,833,225 bp [NCB137/mm9; L:SV40:178,833,225(+)]. The donating investigator reports that homozygous mice have complete absence of Sdccag8 transcript. All homozygous mice exhibit cleft palate, with open palate observed by embryonic day (E)15. Homozygous mice die shortly after birth with complications from cleft palate (cannot suckle). In addition, the donating investigator reports that homozygous mice exhibit preaxial polydactyly and polycystic kidney disease. Hemizygous and homozygous mice of line OVE2161B-CA1C-2 exhibit very light tan coat color and red eyes.Development
A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-BART3 transposon vector used here has (from 5' to 3') an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), an AD2 splice acceptor and polyA sequence, the tyrosinase minigene TyBS, a loxP site, a polyA sequence, a Hox9c-based splice acceptor, and right IR/DR (280 bp). The IR/DR sequences are outward-facing. The 4.1 kb tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyrs-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). This transgene was microinjected into one-cell stage FVB/N embryos. Expression of the tyrosinase minigene results in melanin synthesis, and founder mice (F0) were identified by inspection for pigmentation. F0 mice were assigned an OVE number and then bred with FVB/N mice. Pigmented offspring (F1) with different coat colors were designated as individual sublines (A, B, etc.). F1 mice were bred with FVB/N mice. F2 mice with identical coat colors were then inbred to generate homozygotes. Homozygous mice were found to have cleft palate. In an attempt to generate sublines with additional mutations, hemizygous mice were bred to mice with widespread expression of the SB transposase (CAGGS-SB10 transgenic mice on an FVB/N genetic background). The resulting double transgenic male offspring ("seed males") have the ability to mobilize transposons in their germline; and mobilization of the outward-facing, IR/DR site-flanked transposon results in transposon integration at new sites in the genome (as well as genomic rearrangements including deletions, inversions and translocations). Seed males were bred with FVB/N females, and offspring were screened for new coat colors (i.e.; new integration sites of the transposon). Mice with new transpositions (new F0 mice) were bred to FVB/N mice to establish new lines (CA1, CA2, etc.). F1 mice for each new line were inbred and assessed for phenotype. Hemizygous and homozygous mice of line OVE2161B-CA1C-2 exhibit very light tan coat color and red eyes. Homozygous mice continued to exhibit the cleft palate phenotype. Inverse PCR analysis identified that the original transposon transgene integration site had been retained. The integration site was identified between exons 12-13 of the serologically defined colon cancer antigen 8 gene (Sdccag8) on chromosome 1. The transgene is linked to the (+) strand of DNA at position 178,833,225 bp [NCB137/mm9; L:SV40:178,833,225(+)]. The donating investigator reports the CAGGS-SB10 transgene was bred out of the line prior to sending to The Jackson Laboratory Repository Facebase collection. Upon arrival, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish The Jackson Laboratory Repository Facebase colony.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Facebase: models
View Facebase: models (58 strains)
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Bardet-Biedl Syndrome; BBS (SDCCAG8)
Senior-Loken Syndrome 7; SLSN7 (SDCCAG8)
assigned by genotype
Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove
involves: FVB/N
- mortality/aging
- complete neonatal lethality
- with complications from cleft palate (cannot suckle) (MGI Ref ID J:180503)
- craniofacial phenotype
- cleft palate
- open at E15.5 (MGI Ref ID J:180503)
- limbs/digits/tail phenotype
- preaxial polydactyly (MGI Ref ID J:180503)
- renal/urinary system phenotype
- polycystic kidney (MGI Ref ID J:180503)
- digestive/alimentary phenotype
- cleft palate
- open at E15.5 (MGI Ref ID J:180503)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Craniofacial and Palate Defects
congenital cleft palate
Internal/Organ Defects
kidney
Skeletal Defects
polydactyly
Endocrine Deficiency Research
Kidney Defects
Internal/Organ Research
Kidney Defects
polycystic kidney disease
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove | ||
|---|---|---|---|
| Allele Name | transposon insertion CA1C2, Paul A Overbeek | ||
| Allele Type | Transposon induced | ||
| Common Name(s) | OVE2161B-CA1C-2; | ||
| Mutation Made By | Paul Overbeek, Baylor College of Medicine | ||
| Strain of Origin | FVB/N | ||
| Gene Symbol and Name | Sdccag8, serologically defined colon cancer antigen 8 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | 2700048G21Rik; 5730470G24Rik; BBS16; CCCAP; CCCAP SLSN7; NPHP10; NY-CO-8; RIKEN cDNA 2700048G21 gene; RIKEN cDNA 5730470G24 gene; SLSN7; hCCCAP; | ||
| Molecular Note | A Sleeping Beauty (SB) transposon transgenic approach was used to generate these mice. The pT2-BART3 transposon vector used here has (from 5' to 3') an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site [280 bp]), an AD2 splice acceptor and polyA sequence, the tyrosinase minigene TyBS, a loxP site, a polyA sequence, a Hox9c splice acceptor, and right IR/DR (280 bp). The IR/DR sequences are outward-facing. The 4.1 kb tyrosinase minigene (TyBS) contains the tyrosinase upstream regulatory sequences (2.1 kbp from the BALB/c tyrosinase promoter and the first 65 bp of exon 1), followed by the C57BL/6-derived Tyrs-J cDNA sequence (1.9 kbp including cysteine at amino acid 103, glycine at amino acid 346, and the stop codon with polyA signal). Mice were bred to mice with widespread expression of the SB transposase (CAGGS-SB10 transgenic mice on an FVB/N genetic background). The resulting double transgenic male offspring ("seed males") have the ability to mobilize transposons in their germline; and mobilization of the outward-facing, IR/DR site-flanked transposon results in transposon integration at new sites in the genome (as well as genomic rearrangements including deletions, inversions and translocations). In line OVE2161B.CA1C2, the transposon integrates between exons 12-13 (178,833,225 bp; NCB137/mm9; L:SV40:178,833,225(+)). The absence of transcript expression was confirmed. [MGI Ref ID J:180503] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Sdccag8Tn(sb-Tyr)2161B.CA1C2Ovealternate1, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Overbeek PA. 2012. Direct Data Submission 2012/02/16 MGI Direct Data Submission :. [MGI Ref ID J:180503]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry Homozygous mice die shortly after birth with complications from cleft palate (cannot suckle). When maintaining a live colony, heterozygous mice may be bred to wildtype siblings or to FVB/NJ inbred mice (Stock No. 001800). Mating System Wild-type x Heterozygote (Female x Male) 02-MAR-12 Heterozygote x Wild-type (Female x Male) 02-MAR-12 Diet Information LabDiet® 5K20
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Heterozygous for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove
Price per Pair (US dollars $) Pair Genotype $296.00 Heterozygous for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove x Wild-type for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove $296.00 Wild-type for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove x Heterozygous for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Heterozygous for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove
Price per Pair (US dollars $) Pair Genotype $384.80 Heterozygous for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove x Wild-type for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove $384.80 Wild-type for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove x Heterozygous for Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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