Strain Name:

C.129(B6)-Trp53bp2tm1Cdlo/J

Stock Number:

017615

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Availability:

Repository- Live

Exons 10-17 of the Trp53bp2 gene are disrupted in this strain, abolishing gene function. These mice may be useful for studying tumor development and treatment.

Description

Strain Information

Former Names C.Cg-Trp53bp2tm1Cdlo/J    (Changed: 07-DEC-11 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemWild-type x Heterozygote         (Female x Male)   13-JUN-12
Mating SystemHeterozygote x Wild-type         (Female x Male)   13-JUN-12
Specieslaboratory mouse
GenerationN8+pN1 (06-DEC-13)
Generation Definitions
 
Donating Investigator Charles D Lopez,   Oregon Health and Science University

Description
A loxP-flanked PGK-neo cassette replaces exons 10-17 of the transformation related protein 53 binding protein 2 (Trp53bp2) gene, abolishing gene function in this strain. ASPP2, encoded by the Trp53bp2 gene, is a pro-apoptotic protein which also inhibits cell growth through interactions with other regulatory molecules including members of the p53 family. The disrupted exons in this mutant include codons for the ankyrin repeat and SH3 domain, required for interaction with p53 family members. Homozygotes exhibit embryonic lethality by embryonic day 6.5. Heterozygous mice are viable, fertile and exhibit increased incidence of spontaneous tumors as they age. γ-irradiated heterozygotes exhibit an increased incidence of high-grade thymic T cell lymphomas and attenuated apoptotic response in primary thymocytes.

Development
A targeting vector was designed to replace exons 10-17 of the transformation related protein 53 binding protein 2 (Trp53bp2) gene with a loxP-flanked PGK-neo cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and the resulting chimeric males were bred to C57BL/6 females generate a colony of ASPP2+/- mice. These mice were then backcrossed to C57BL/6J mice and then to BALB/cJ for at least 8 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ (Stock No. 000651) for at least one generation.

Control Information

  Control
   Wild-type from the colony
   000651 BALB/cJ (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Trp53bp2tm1Cdlo/Trp53bp2+

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • tumorigenesis
  • increased tumor incidence
    • increased tumor incidence but the spectrum of tumor types is similar to controls   (MGI Ref ID J:146764)
    • increased incidence of tumors by ionizing radiation induction
      • unlike wild-type controls, mice develop tumors following exposure to 6.0 Gy or 10.5 Gy of gamma-radiation   (MGI Ref ID J:146764)
      • induced tumors are high grade lymphomas   (MGI Ref ID J:146764)
  • immune system phenotype
  • abnormal T cell physiology
    • attenuated T cell apoptotic response following gamma-irradiation   (MGI Ref ID J:146764)
  • cellular phenotype
  • abnormal cell cycle checkpoint function
    • attenuated G0/G1 checkpoint function following gamma-irradiation   (MGI Ref ID J:146764)
  • hematopoietic system phenotype
  • abnormal T cell physiology
    • attenuated T cell apoptotic response following gamma-irradiation   (MGI Ref ID J:146764)

Trp53bp2tm1Cdlo/Trp53bp2tm1Cdlo

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • mortality/aging
  • complete embryonic lethality
    • unable to characterize homozygous embryos at E6.5   (MGI Ref ID J:146764)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Apoptosis Research

Cancer Research
Increased Tumor Incidence
      Lymphomas

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Trp53bp2tm1Cdlo
Allele Name targeted mutation 1, Charles D Lopez
Allele Type Targeted (knock-out)
Common Name(s) ASPP2-;
Mutation Made By Charles Lopez,   Oregon Health and Science University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Trp53bp2, transformation related protein 53 binding protein 2
Chromosome 1
Gene Common Name(s) 53BP2; AI746547; ASPP2; BBP; P53BP2; PPP1R13A; X98550; expressed sequence AI746547; expressed sequence X98550;
Molecular Note Exons 10 - 17 were replaced with a floxed neo cassette via homologous recombination. Western blot analysis indicates about a 2-fold reduction in protein expression in MEFs, thymus and liver from heterozygous mice. [MGI Ref ID J:146764]

Genotyping

Genotyping Information

Genotyping Protocols

Trp53bp2tm1Cdlo,

Separated MCA


Generic Neo Melt Curve Analysis, Melt Curve Analysis
Trp53bp2tm1Cdlo, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Kampa KM; Acoba JD; Chen D; Gay J; Lee H; Beemer K; Padiernos E; Boonmark N; Zhu Z; Fan AC; Bailey AS; Fleming WH; Corless C; Felsher DW; Naumovski L; Lopez CD. 2009. Apoptosis-stimulating protein of p53 (ASPP2) heterozygous mice are tumor-prone and have attenuated cellular damage-response thresholds. Proc Natl Acad Sci U S A 106(11):4390-5. [PubMed: 19251665]  [MGI Ref ID J:146764]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to BALB/cJ inbred mice (Stock No. 000651). Homozygotes exhibit embryonic lethality by day E6.5.
Mating SystemWild-type x Heterozygote         (Female x Male)   13-JUN-12
Heterozygote x Wild-type         (Female x Male)   13-JUN-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHeterozygous for Trp53bp2tm1Cdlo  
Price per Pair (US dollars $)Pair Genotype
$302.00Heterozygous for Trp53bp2tm1Cdlo x Wild-type for Trp53bp2tm1Cdlo  
$302.00Wild-type for Trp53bp2tm1Cdlo x Heterozygous for Trp53bp2tm1Cdlo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHeterozygous for Trp53bp2tm1Cdlo  
Price per Pair (US dollars $)Pair Genotype
$392.60Heterozygous for Trp53bp2tm1Cdlo x Wild-type for Trp53bp2tm1Cdlo  
$392.60Wild-type for Trp53bp2tm1Cdlo x Heterozygous for Trp53bp2tm1Cdlo  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   Wild-type from the colony
   000651 BALB/cJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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