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| These Flk1-TVA transgenic mice contain the mouse kinase insert domain protein receptor (Kdr) promoter driving expression of an avian specific retroviral receptor (TVA) derived from quail. These TVA mice may be useful for studying genes of interest involved in angiogenesis. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1
Generation DefinitionsDonating Investigator Elena Pasquale, Sanford-Burnham Medical Research Institu Description
These Flk1-TVA transgenic mice contain the mouse kinase insert domain protein receptor (Kdr) promoter driving expression of an avian specific retroviral receptor (TVA) derived from quail. FLK1 is a receptor tyrosine kinase expressed on proliferating adult and embryonic endothelial cells, and is involved in the development of angiogenic vasculature. Hemizygous mice are viable and fertile. Expression of TVA in these mice makes them highly susceptible to infection by RCAS (replication-competent, avian leukosis virus LTR, splice acceptor) gene delivery machinery. The RCAS integrates its genetic information into the host DNA, promoting a high level of viral transcription. The insertion of a gene of interest into multicloning site within RCAS-derived constructs allows for its cell specific constitutive expression in vascular epithelial cells. These TVA mice may be useful for studying genes of interest involved in angiogenesis.Development
The Flk1-TVA transgene was designed with the kinase insert domain protein receptor (Kdr) promoter driving expression of an avian specific retroviral receptor (TVA) gene derived from quail. The transgene was microinjected into fertilized FVB/N oocytes, and mice from founder line 1026 were then bred to FVB/NJ mice to establish a colony. Upon arrival at The Jackson Laboratory, mice were bred to FVB/NJ inbred mice (Stock No. 001800) for one generation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Kdr
002938 B6.129-Kdrtm1Jrt/J 018542 FVB-Tg(Kdr-mCherry)1Medi/J 008685 FVB-Tg(tetO-Kdr*)4377.5Rwng/J 018976 STOCK Kdrtm1(cre)Sato/J 017006 STOCK Kdrtm2.1Jrt/J 018977 STOCK Kdrtm2Sato/J View Strains carrying other alleles of Kdr (6 strains)
015805 B6.Cg-Tg(UBC-GFP,-TVA)1Clc/J 015806 B6.Cg-Tg(UBC-GFP,-TVA)2Clc/J 015807 B6.Cg-Tg(UBC-GFP,-TVA)3Clc/J 015808 B6.Cg-Tg(UBC-TVA)1Clc/J 011103 STOCK Olig2tm2(TVA,cre)Rth/J 003528 STOCK Tg(GFAP-TVA)5Hev/J 003529 STOCK Tg(NES-TVA)J12Ech/J
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
vasculature
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Kdr-TVA)1026Ebp | ||
|---|---|---|---|
| Allele Name | transgene insertion 1026, Elena Pasquale | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Flk1-TVA; | ||
| Mutation Made By | Elena Pasquale, Sanford-Burnham Medical Research Institu | ||
| Strain of Origin | FVB/N | ||
| Expressed Gene | TVA, subgroup A avian leukosis virus receptor, quail | ||
| Promoter | Kdr, kinase insert domain protein receptor, mouse, laboratory | ||
| General Note | Proliferating endothelial cells of new vasculature induced by extracellular matrix implants or by tumors in adult mice bearing this transgene are susceptible to infection by Rous sarcoma virus derived RCAS (replication-competent, avian leukosis virus LTR, splice acceptor) avian retroviral expression vectors, demonstrated by expression specifically in these cells of green fluorescent protein from one such vector. (J:134089) | ||
| Molecular Note | A 786-nucleotide cDNA (nucleotides 34-819 of pg800) encoding the quail receptor (TVA) for avian leukosis viruses of subgroup A [ALV(A)] is expressed under the control of regulatory sequences from the mouse Kdr (kinase insert domain protein receptor) gene: a -640 bp to +299 bp genomic DNA fragment containing the promoter and a 2.3 kb fragment containing the endothelium-specific enhancer from the first intron. Embryonic day (E) 10.5 embryos of this line were shown by whole mount in situ hybridization to express TVA mRNA selectively in the developing vasculature, in a pattern similar to that of endogenous Kdr mRNA. Only endothelial cells of new vasculature induced in adult mice exhibit strong TVA immunoreactivity. [MGI Ref ID J:134089] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Kdr-TVA)1026Ebp, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Vervoort VS; Lu M; Valencia F; Lesperance J; Breier G; Oshima R; Pasquale EB. 2008. A novel Flk1-TVA transgenic mouse model for gene delivery to angiogenic vasculature. Transgenic Res 17(3):403-15. [PubMed: 18027100] [MGI Ref ID J:134089]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carriers) from the colony or to FVB/NJ inbred mice (Stock No. 001800).
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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