Strain Name:

C.129S6(Cg)-Ccl11tm1Mer/J

Stock Number:

017618

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These Ccl11 knockout mice exhibit a reduced total eosinophil count in peripheral blood and may be useful for studying eosinophilia and inflammatory disorders.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Marc Rothenberg,   Cincinnati Children's Hospital

Description
Mice homozygous for the Ccl11tm1Mer allele contain a neomycin resistance cassette that replaces exon 2 and part of exon 3, abolishing gene expression. CCL11 (or eotaxin) is an eosinphil specific C-C chemokine involved in eosinophil recruitment. Mice deficient in eotaxin exhibit a reduced total eosinophil count in peripheral blood. Following antigen challenge, eosinophils are significantly reduced in the early, but not late, phase of the inflammatory response. Homozygotes are viable, fertile, normal in size, and do not display any gross physical abnormalities. These mice may be useful for studying eosinophilia in peripheral blood and inflammatory disorders.

Development
A targeting vector containing a PGKneo cassette was used to disrupt all of exon 2, which encodes the mature protein, and most of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to outbred mice and maintained on a mixed background.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Asthma, Susceptibility to   (CCL11)
Human Immunodeficiency Virus Type 1, Susceptibility to   (CCL11)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ccl11tm1Mer/Ccl11tm1Mer

        involves: 129S6/SvEvTac * NIH Black Swiss
  • immune system phenotype
  • decreased eosinophil cell number
    • total eosinophil count is reduced compared with wild-type mice   (MGI Ref ID J:38945)
  • impaired eosinophil recruitment
    • mutants exhibit reduced early, but not late, eosinophil recruitment after antigen challenge in models of asthma and stromal keratitis   (MGI Ref ID J:38945)
  • hematopoietic system phenotype
  • decreased eosinophil cell number
    • total eosinophil count is reduced compared with wild-type mice   (MGI Ref ID J:38945)
  • impaired eosinophil recruitment
    • mutants exhibit reduced early, but not late, eosinophil recruitment after antigen challenge in models of asthma and stromal keratitis   (MGI Ref ID J:38945)

Ccl11tm1Mer/Ccl11tm1Mer

        129S.129S6-Ccl11tm1Mer
  • immune system phenotype
  • abnormal immune system physiology
    • mutant mice have markedly fewer eosinophils in the jejunum than do wild-type controls   (MGI Ref ID J:102034)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ccl11tm1Mer
Allele Name targeted mutation 1, Marc E Rothenberg
Allele Type Targeted (Null/Knockout)
Common Name(s) Eot-; eotaxin-; eotaxin-1;
Mutation Made By Marc Rothenberg,   Cincinnati Children's Hospital
Strain of Origin129S6/SvEvTac
Gene Symbol and Name Ccl11, chemokine (C-C motif) ligand 11
Chromosome 11
Gene Common Name(s) SCYA11; Scya11; eotaxin; small inducible cytokine A11;
Molecular Note All of exon 2 and the majority of exon 3 were replaced by a neomycin selection cassette. In addition to containing the 3' UTR, the deleted region encoded most of the mature protein. Transcript was undetected in homozygous mutant mice via Northern blot analysis of skin and lung RNA (the latter of which was isolated from mice treated with and subsequently challenged with OVA).

Genotyping

Genotyping Information

Genotyping Protocols

Ccl11tm1Mer,

Separated MCA


Ccl11tm1Mer, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Rothenberg ME; MacLean JA; Pearlman E; Luster AD; Leder P. 1997. Targeted disruption of the chemokine eotaxin partially reduces antigen-induced tissue eosinophilia. J Exp Med 185(4):785-90. [PubMed: 9034156]  [MGI Ref ID J:38945]

Additional References

Ccl11tm1Mer related

Ahrens R; Waddell A; Seidu L; Blanchard C; Carey R; Forbes E; Lampinen M; Wilson T; Cohen E; Stringer K; Ballard E; Munitz A; Xu H; Lee N; Lee JJ; Rothenberg ME; Denson L; Hogan SP. 2008. Intestinal macrophage/epithelial cell-derived CCL11/eotaxin-1 mediates eosinophil recruitment and function in pediatric ulcerative colitis. J Immunol 181(10):7390-9. [PubMed: 18981162]  [MGI Ref ID J:141072]

Boehme SA; Lio FM; Sikora L; Pandit TS; Lavrador K; Rao SP; Sriramarao P. 2004. Cutting edge: serotonin is a chemotactic factor for eosinophils and functions additively with eotaxin. J Immunol 173(6):3599-603. [PubMed: 15356103]  [MGI Ref ID J:92758]

Dixon H; Blanchard C; Deschoolmeester ML; Yuill NC; Christie JW; Rothenberg ME; Else KJ. 2006. The role of Th2 cytokines, chemokines and parasite products in eosinophil recruitment to the gastrointestinal mucosa during helminth infection. Eur J Immunol 36(7):1753-63. [PubMed: 16783848]  [MGI Ref ID J:115793]

Forbes E; Murase T; Yang M; Matthaei KI; Lee JJ; Lee NA; Foster PS; Hogan SP. 2004. Immunopathogenesis of experimental ulcerative colitis is mediated by eosinophil peroxidase. J Immunol 172(9):5664-75. [PubMed: 15100311]  [MGI Ref ID J:89683]

Fulkerson PC; Fischetti CA; McBride ML; Hassman LM; Hogan SP; Rothenberg ME. 2006. A central regulatory role for eosinophils and the eotaxin/CCR3 axis in chronic experimental allergic airway inflammation. Proc Natl Acad Sci U S A 103(44):16418-23. [PubMed: 17060636]  [MGI Ref ID J:115273]

Fulkerson PC; Fischetti CA; Rothenberg ME. 2006. Eosinophils and CCR3 regulate interleukin-13 transgene-induced pulmonary remodeling. Am J Pathol 169(6):2117-26. [PubMed: 17148674]  [MGI Ref ID J:116219]

Gouon-Evans V; Rothenberg ME; Pollard JW. 2000. Postnatal mammary gland development requires macrophages and eosinophils. Development 127(11):2269-82. [PubMed: 10804170]  [MGI Ref ID J:110688]

Hogan SP; Mishra A; Brandt EB; Foster PS; Rothenberg ME. 2000. A critical role for eotaxin in experimental oral antigen-induced eosinophilic gastrointestinal allergy. Proc Natl Acad Sci U S A 97(12):6681-6. [PubMed: 10841566]  [MGI Ref ID J:62718]

Hogan SP; Mishra A; Brandt EB; Royalty MP; Pope SM; Zimmermann N; Foster PS; Rothenberg ME. 2001. A pathological function for eotaxin and eosinophils in eosinophilic gastrointestinal inflammation. Nat Immunol 2(4):353-60. [PubMed: 11276207]  [MGI Ref ID J:125657]

Johnson TR; Rothenberg ME; Graham BS. 2008. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein. J Leukoc Biol 84(3):748-59. [PubMed: 18519743]  [MGI Ref ID J:138168]

Knott ML; Matthaei KI; Foster PS; Dent LA. 2009. The roles of eotaxin and the STAT6 signalling pathway in eosinophil recruitment and host resistance to the nematodes Nippostrongylus brasiliensis and Heligmosomoides bakeri. Mol Immunol 46(13):2714-22. [PubMed: 19535141]  [MGI Ref ID J:151720]

Mattes J; Yang M; Mahalingam S; Kuehr J; Webb DC; Simson L; Hogan SP; Koskinen A; McKenzie AN; Dent LA; Rothenberg ME; Matthaei KI; Young IG; Foster PS. 2002. Intrinsic defect in T cell production of interleukin (IL)-13 in the absence of both IL-5 and eotaxin precludes the development of eosinophilia and airways hyperreactivity in experimental asthma. J Exp Med 195(11):1433-44. [PubMed: 12045241]  [MGI Ref ID J:116794]

Matthews AN; Friend DS; Zimmermann N; Sarafi MN; Luster AD; Pearlman E; Wert SE; Rothenberg ME. 1998. Eotaxin is required for the baseline level of tissue eosinophils. Proc Natl Acad Sci U S A 95(11):6273-8. [PubMed: 9600955]  [MGI Ref ID J:120566]

Mishra A; Hogan SP; Brandt EB; Rothenberg ME. 2002. IL-5 promotes eosinophil trafficking to the esophagus. J Immunol 168(5):2464-9. [PubMed: 11859139]  [MGI Ref ID J:74724]

Miyazaki D; Nakamura T; Ohbayashi M; Kuo CH; Komatsu N; Yakura K; Tominaga T; Inoue Y; Higashi H; Murata M; Takeda S; Fukushima A; Liu FT; Rothenberg ME; Ono SJ. 2009. Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade. Int Immunol 21(2):187-201. [PubMed: 19147836]  [MGI Ref ID J:144492]

Pope SM; Brandt EB; Mishra A; Hogan SP; Zimmermann N; Matthaei KI; Foster PS; Rothenberg ME. 2001. IL-13 induces eosinophil recruitment into the lung by an IL-5- and eotaxin-dependent mechanism. J Allergy Clin Immunol 108(4):594-601. [PubMed: 11590387]  [MGI Ref ID J:96752]

Pope SM; Zimmermann N; Stringer KF; Karow ML; Rothenberg ME. 2005. The eotaxin chemokines and CCR3 are fundamental regulators of allergen-induced pulmonary eosinophilia. J Immunol 175(8):5341-50. [PubMed: 16210640]  [MGI Ref ID J:102034]

Rajavelu P; Rayapudi M; Moffitt M; Mishra A; Mishra A. 2012. Significance of para-esophageal lymph nodes in food or aeroallergen-induced iNKT cell-mediated experimental eosinophilic esophagitis. Am J Physiol Gastrointest Liver Physiol 302(7):G645-54. [PubMed: 22207581]  [MGI Ref ID J:183954]

Sharkhuu T; Matthaei KI; Forbes E; Mahalingam S; Hogan SP; Hansbro PM; Foster PS. 2006. Mechanism of interleukin-25 (IL-17E)-induced pulmonary inflammation and airways hyper-reactivity. Clin Exp Allergy 36(12):1575-83. [PubMed: 17177681]  [MGI Ref ID J:135913]

Simons JE; Rothenberg ME; Lawrence RA. 2005. Eotaxin-1-regulated eosinophils have a critical role in innate immunity against experimental Brugia malayi infection. Eur J Immunol 35(1):189-97. [PubMed: 15593125]  [MGI Ref ID J:95225]

Simson L; Ellyard JI; Dent LA; Matthaei KI; Rothenberg ME; Foster PS; Smyth MJ; Parish CR. 2007. Regulation of carcinogenesis by IL-5 and CCL11: a potential role for eosinophils in tumor immune surveillance. J Immunol 178(7):4222-9. [PubMed: 17371978]  [MGI Ref ID J:122672]

Takeda A; Baffi JZ; Kleinman ME; Cho WG; Nozaki M; Yamada K; Kaneko H; Albuquerque RJ; Dridi S; Saito K; Raisler BJ; Budd SJ; Geisen P; Munitz A; Ambati BK; Green MG; Ishibashi T; Wright JD; Humbles AA; Gerard CJ; Ogura Y; Pan Y; Smith JR; Grisanti S; Hartnett ME; Rothenberg ME; Ambati J. 2009. CCR3 is a target for age-related macular degeneration diagnosis and therapy. Nature 460(7252):225-30. [PubMed: 19525930]  [MGI Ref ID J:150352]

Waddell A; Ahrens R; Steinbrecher K; Donovan B; Rothenberg ME; Munitz A; Hogan SP. 2011. Colonic eosinophilic inflammation in experimental colitis is mediated by Ly6C(high) CCR2(+) inflammatory monocyte/macrophage-derived CCL11. J Immunol 186(10):5993-6003. [PubMed: 21498668]  [MGI Ref ID J:173219]

Wang A; Fernando M; Leung G; Phan V; Smyth D; McKay DM. 2010. Exacerbation of oxazolone colitis by infection with the helminth Hymenolepis diminuta: involvement of IL-5 and eosinophils. Am J Pathol 177(6):2850-9. [PubMed: 21037078]  [MGI Ref ID J:167635]

Yang M; Hogan SP; Henry PJ; Matthaei KI; McKenzie AN; Young IG; Rothenberg ME; Foster PS. 2001. Interleukin-13 mediates airways hyperreactivity through the IL-4 receptor-alpha chain and STAT-6 independently of IL-5 and eotaxin. Am J Respir Cell Mol Biol 25(4):522-30. [PubMed: 11694459]  [MGI Ref ID J:114421]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhile maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   None Available
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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