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| Blk (B lymphoid kinase) knockout mice show no overt immune system defect. However, when combined with other redundant Src-family gene mutations, a role in NF-κB activation and B cell development can be seen. | |||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Alexander Tarakhovsky, The Rockefeller University Description
Blk (B lymphoid kinase) is a Src-family protein tyrosine kinase specifically associated with B cells. Expression is eliminated in this knockout allele, as determined by Northern and Western blots of splenic B cells from homozygous mice. Although it is typically expressed during B cell development (in bone marrow) and is present in mature cells at high levels, there is no overt immune system defect in these animals. Development of B and T cells is unaltered. Both in vitro and in vivo B cell responses appear unchanged. Humoral immune responses of B cells to both T cell-dependent and -independent antigens are normal. Homozygotes are viable and fertile.When combined with both Fyn (e.g. Stock No. 012468) and Lyn knockout mutations (sharing some redundant functionality), a role for the Blk gene in NF-κB activation during B cell development becomes apparent.
Development
A neomycin cassette inserted in reverse transcriptional orientation replaces portions of intron 7 and exon 8. The mutation was created in E14-1.1 129P2/OlaHsd-derived embryonic stem (ES) cells. The strain was backcrossed to C57BL/6 for 8 generations by the donating laboratory.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Related Disease (OMIM) Terms
Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Maturity-Onset Diabetes of the Young, Type 11; MODY11 (BLK)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Blktm1Tara/Blktm1Tara
involves: 129P2/OlaHsd
- normal phenotype
- no abnormal phenotype detected
- homozygous mice exhibit no discernable phenotype; mice are viable and fertile with normal B cell development and immune responses (MGI Ref ID J:60221)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
B cell defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Blktm1Tara | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Alexander Tarakhovsky | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | blk-; | ||
| Mutation Made By | Angela Santana, Rockefeller University | ||
| Strain of Origin | 129P2/OlaHsd | ||
| Gene Symbol and Name | Blk, B lymphoid kinase | ||
| Chromosome | 14 | ||
| Gene Common Name(s) | MODY11; | ||
| General Note | ES cell line = E14-1.1 | ||
| Molecular Note | Sequences encoding the essential portion of the kinase domain in exon 8 were replaced with a neomycin resistance gene via homologous recombination. Gene expression was not detected in splenic B-cells from homozygous mutant animals via Northern blot, but RT-PCR analysis detected transcripts containing exons 1-4. However, Western blot using antibodies directed against the N-terminal region of the protein did not detect any translation product from the truncated transcript. [MGI Ref ID J:60221] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Blktm1Tara, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Texido G; Su IH; Mecklenbrauker I; Saijo K; Malek SN; Desiderio S; Rajewsky K; Tarakhovsky A. 2000. The B-cell-specific Src-family kinase Blk is dispensable for B-cell development and activation. Mol Cell Biol 20(4):1227-33. [PubMed: 10648608] [MGI Ref ID J:60221]
Additional References
Blktm1Tara relatedChan VW; Mecklenbrauker I; Su I; Texido G; Leitges M; Carsetti R; Lowell CA; Rajewsky K; Miyake K; Tarakhovsky A. 1998. The molecular mechanism of B cell activation by toll-like receptor protein RP-105. J Exp Med 188(1):93-101. [PubMed: 9653087] [MGI Ref ID J:115145]
Laird RM; Laky K; Hayes SM. 2010. Unexpected role for the B cell-specific Src family kinase B lymphoid kinase in the development of IL-17-producing gammadelta T cells. J Immunol 185(11):6518-27. [PubMed: 20974990] [MGI Ref ID J:167365]
Saijo K; Schmedt C; Su IH; Karasuyama H; Lowell CA; Reth M; Adachi T; Patke A; Santana A; Tarakhovsky A. 2003. Essential role of Src-family protein tyrosine kinases in NF-kappaB activation during B cell development. Nat Immunol 4(3):274-9. [PubMed: 12563261] [MGI Ref ID J:115080]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Homozygotes or heterozygotes may be bred.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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