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| These NCoRlox mutant mice possess loxP sites flanking exons 37-40 of the nuclear receptor co-repressor 1 (Ncor1) gene. This strain may be useful for studying the role of NCOR1 on regulation of nuclear receptor signaling. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +pN1
Generation DefinitionsDonating Investigator Anthony Hollenberg, Beth Israel Deaconess Medical Center Description
These NCoRlox mutant mice possess loxP sites flanking exons 37-40 of the nuclear receptor co-repressor 1 (Ncor1) gene. Exons 37-40 encode two of three 5' receptor interacting domains (RIDs). NCOR1 is recruited through interactions of its RIDs to nuclear receptors, such as retinoic-acid and thyroid-hormone receptors (TR), where it acts as a transcription repressor. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exons 37-40 deleted in the cre-expressing tissues. For example, when bred to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) deletion of the two most 5' RIDs (N2 and N3) in the liver results in the inability of NCOR1 to bind to the TR. This strain may be useful for studying the role of NCOR1 on regulation of nuclear receptor signaling.Development
A targeting vector was designed to insert a loxP site upstream of exon 37 followed by a second loxP site and a frt-flanked neomycin resistance (neo) cassette downstream of exon 40 of the nuclear receptor co-repressor 1 (Ncor1) gene. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric males were bred to C57BL/6NTac females. The resulting offspring were bred with 129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J mice (Stock No. 003946) to delete the neo cassette, and progeny were crossed to remove the Flp-expressing transgene. The mice were subsequently bred to B6.Cg-Tg(Alb-cre)21Mgn/J mice (Stock No. 003574) and B6.Cg-Tg(UBC-cre/ERT2)1Ejb/J mice (Stock No. 008085) for experimental purposes. The DI reports that the Cre-expressing transgene has been removed. These mice were maintained on a mixed background. Upon arrival, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Phenotype
Phenotype Information
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Ncor1tm1Anh/Ncor1tm1Anh Tg(Alb-cre)21Mgn/0
involves: 129S4/SvJae * 129S4/SvJaeSor * C57BL/6 * DBA (conditional)
- homeostasis/metabolism phenotype
- decreased circulating LDL cholesterol level
- PTU treated mice exhibit decreased serum LDL cholesterol levels compared to in similarly treated Ncor1tm1Anh control mice (MGI Ref ID J:142045)
- decreased circulating thyroxine level
- male mice exhibit a slight decrease in plasma T4 levels compared to in Ncor1tm1Anh control mice (MGI Ref ID J:142045)
This mouse can be used to support research in many areas including:
Cell Biology Research
Signal Transduction
Transcriptional Regulation
Endocrine Deficiency Research
Thyroid Defects
Internal/Organ Research
Thyroid Defects
Mouse/Human Gene Homologs
hypothyroidism
Research Tools
Cre-lox System
loxP-flanked Sequences
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ncor1tm1Anh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Anthony N Hollenberg | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | NCoRlox; | ||
| Mutation Made By | Anthony Hollenberg, Beth Israel Deaconess Medical Center | ||
| Strain of Origin | 129S4/SvJae | ||
| Gene Symbol and Name | Ncor1, nuclear receptor co-repressor 1 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | 5730405M06Rik; A230020K14Rik; N-CoR; N-CoR1; RIKEN cDNA 5730405M06 gene; RIKEN cDNA A230020K14 gene; Rxrip13; TRAC1; hN-CoR; mKIAA1047; retinoid X receptor interacting protein 13; | ||
| Molecular Note | A loxP site was inserted upstream of exon 38 and an frt flanked neo cassette with a 5' loxP site was inserted downstream of exon 41. Germ line, flp mediated recombination was used to remove the neo cassette leaving exons 38 through 41 floxed. These exons encode two of the three receptor interacting domains (N2 and N3). [MGI Ref ID J:142045] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ncor1tm1Anh, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Astapova I; Lee LJ; Morales C; Tauber S; Bilban M; Hollenberg AN. 2008. The nuclear corepressor, NCoR, regulates thyroid hormone action in vivo. Proc Natl Acad Sci U S A 105(49):19544-9. [PubMed: 19052228] [MGI Ref ID J:142045]
Additional References
Ncor1tm1Anh relatedCosta-e-Sousa RH; Astapova I; Ye F; Wondisford FE; Hollenberg AN. 2012. The thyroid axis is regulated by NCoR1 via its actions in the pituitary. Endocrinology 153(10):5049-57. [PubMed: 22878400] [MGI Ref ID J:191436]
Fozzatti L; Lu C; Kim DW; Park JW; Astapova I; Gavrilova O; Willingham MC; Hollenberg AN; Cheng SY. 2011. Resistance to thyroid hormone is modulated in vivo by the nuclear receptor corepressor (NCOR1). Proc Natl Acad Sci U S A 108(42):17462-7. [PubMed: 21987803] [MGI Ref ID J:177419]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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