Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote         (Female x Male)   26-JAN-13 Species laboratory mouse Generation N5+pN1 (05-APR-13) Generation Definitions   Donating Investigator Gary A. Silverman,   University of Pittsburgh

Description
The mouse Serpinb3a gene is similar to the human SERPINB4 and SERPINB3 genes, which have been associated with allergic reactions and asthma. Mouse Serpinb3a inhibits both serine and cysteine proteinases. These mice carry a null targeted mutation of Serpinb3a; exon 8 is disrupted. No gene product (mRNA) is detected by RT-PCR analysis of homozygotes. Serpinb3a-deficient mice are less sensitive to house dust mite-induced airway hyper-responsiveness, with reduced goblet cell hyperplasia and mucus production when compared to wildtype controls. IL13-induced airway hyper-responsiveness is reduced in homozygotes, with a reduced number of inflammatory cells in the bronchoalveolar lavage fluid. Mice that are homozygous for the targeted mutation are viable and fertile.

Development
A targeting vector containing a NEO cassette was used to disrupt exon 8. The construct was electroporated into unspecified 129 (129/SV) derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to BALB/cAnNCrl mice, and then backcrossed to BALB/cAnNCrl for at least 5 generations. Upon arrival at The Jackson Laboratory, the mice were crossed to BALB/cByJ (Stock No. 001026) at least once to establish the colony.

Control Information

	Control
	Wild-type siblings from the colony
	001026 BALB/cByJ
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Serpinb3a^tm1Gsil/Serpinb3a^tm1Gsil

        C.129-Serpinb3a^tm1Gsil

• respiratory system phenotype
• decreased airway responsiveness
  • house dust mite- or IL13-induced airway hyperresponsiveness is attenuated   (MGI Ref ID J:189949)
  • however, house dust mite-induced allergen sensitization and inflammation are normal   (MGI Ref ID J:189949)
• decreased respiratory mucosa goblet cell number
  • following house dust-mite challenge   (MGI Ref ID J:189949)
• immune system phenotype
• decreased susceptibility to type I hypersensitivity reaction
  • mice exposed to house dust mite exhibit attenuated airway hyperresponsiveness and reduced mucus-producing goblet cell hyperplasia compared with wild-type mice   (MGI Ref ID J:189949)
  • IL13-treated mice exhibit attenuated airway hyperresponsiveness, reduced inflammatory cells in the bronchoalveolar lavage fluid and reduced mucin production compared with wild-type mice   (MGI Ref ID J:189949)
  • however, house dust mite-induced allergen sensitization and inflammation are normal   (MGI Ref ID J:189949)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
Inflammation
      Asthma

Research Tools
Immunology and Inflammation Research

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Serpinb3atm1Gsil
Allele Name		targeted mutation 1, Gary A Silverman
Allele Type		Targeted (knock-out)
Strain of Origin		129
Gene Symbol and Name		Serpinb3a, serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3A
Chromosome		1
Gene Common Name(s)		LEUPIN; PI11; RGD1562868; SCCA-2; SCCA1; SCCA2; Serpinb4; Sqn5;
Molecular Note		A targeting vector containing a NEO cassette replaced most of exon 8. RT-PCR confirmed the absence of transcript expression. [MGI Ref ID J:189949]

Genotyping

Genotyping Information

Genotyping Protocols

Serpinb3a^tm1Gsil alternate1, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sivaprasad U; Askew DJ; Ericksen MB; Gibson AM; Stier MT; Brandt EB; Bass SA; Daines MO; Chakir J; Stringer KF; Wert SE; Whitsett JA; Le Cras TD; Wills-Karp M; Silverman GA; Khurana Hershey GK. 2011. A nonredundant role for mouse Serpinb3a in the induction of mucus production in asthma. J Allergy Clin Immunol 127(1):254-61, 261.e1-6. [PubMed: 21126757]  [MGI Ref ID J:189949]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes.
Mating System	Heterozygote x Heterozygote         (Female x Male)   26-JAN-13

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

 

This strain is currently Under Development - Now Accepting Orders.
Estimated Available for Distribution Date: 17-JUN-13

Please note: You may now place orders for this strain although it is not yet ready for distribution. Estimated available for distribution dates are provided to keep customers better informed on strains under development. Please note that our Colony Managers routinely monitor the target date and edit it based on breeding performance and other factors. The length of time it takes to make a new strain available for distribution depends on genotype, age, number of animals sent by the Donating Investigator, breeding performance, additional strain development (backcrossing, making homozygous), and anticipated demand for the strain.

Control Information

	Control
	Wild-type siblings from the colony
	001026 BALB/cByJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Terms of Use

Terms of Use

General Terms and Conditions

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