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| These inducible calcium sensing GCaMP2 transgenic mice allow transcranial imaging of neurons in live animals. | |||||||||||||||||||
- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAY-12 Mating System Hemizygote x Noncarrier (Female x Male) 02-MAY-12 Species laboratory mouse Generation N4+
Generation DefinitionsDonating Investigator C Ron Yu, Stowers Institute for Medical Research Description
This inducible calcium sensor transgene contains a conditional eGFP-calmodulin fusion protein, GCaMP2. Hemizygotes are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. When bred with transgenic mice expressing a tetracycline transactivator (tTA) GCAMP2, expression is observed in cells of double mutant offspring. An increase of green fluorescence can be observed as the result of elevated intracellular calcium. For example, when bred to mice expressing the mouse olfactory marker protein (OMP) driving tTA (Stock No. 017754), GCAMP2 expression is localized in the vomeronasal neurons and main olfactory sensory neurons, which detect olfactory chemical stimuli. These chemical messages are detected by their binding to G protein-coupled receptors. The receptors activation elevates intracellular calcium, which results in an increase of fluorescence in specific neurons. These mice may be useful for slice preparation imaging or transcranial imaging of activated neurons in live animals.Development
This transgene contains a calcium-sensing molecule, GCaMP2, under the transcriptional control of the tetracycline-responsive promoter element (tetO). GCaMP2 consists of, from 5' to 3', a 13-residue peptide of myosin light chain kinase (M13), a circularly permutated eGFP interrupted at residue 145, calmodulin (CaM), and a polyA recognition sequence. Mutations resulting in increased brightness (D180Y and V93I), thermal stability (V163A, and S175G) and GFP dimerization prevention (A206K) were introduced. This construct was injected into fertilized (CBAxFVB)F1 oocytes and mice from founder line 12i were bred to C57BL/6 mice to establish a colony. Transgenic offspring were bred to C57BL/6 for at least 3 generations. Upon arrival at The Jackson Laboratory, mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Calm1
012477 STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J View Strains carrying other alleles of Calm1 (1 strain)
012477 STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Aortic Aneurysm, Familial Thoracic 7; AAT7 (MYLK)
Ventricular Tachycardia, Catecholaminergic Polymorphic, 4; CPVT4 (CALM1)
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Optogenetic and Pharmacogenetic tools
Calcium sensor expressing strains
Tet Expression System
tTA/rtTA Responsive Strains
Research Tools
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(tetO-GCaMP2)12IRyu | ||
|---|---|---|---|
| Allele Name | transgene insertion 12I, Ron Yu | ||
| Allele Type | Transgenic (Reporter) | ||
| Strain of Origin | (CBA x FVB)F1 | ||
| Site of Expression | When bred to mice expressing a tetracycline transactivator (tTA) or reverse transactivator (rtTA), doxycycline induction results in the expression of a GFP-calmodulin fusion protein, GCaMP2, in transactivator expressing tissues of the offspring. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Calm1, calmodulin 1, mouse, laboratory | ||
| Expressed Gene | Mylk, myosin, light polypeptide kinase, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | This transgene contains a calcium-sensing molecule, GCaMP2, under the transcriptional control of the tetracycline-responsive promoter element (tetO). GCaMP2 consists of, from 5' to 3', a 13-residue peptide of myosin light chain kinase (M13), a circularlypermutated eGFP interrupted at residue 145, calmodulin (CaM), and a polyA recognition sequence. Mutations resulting in increased brightness (D180Y and V93I), thermal stability (V163A, and S175G) and GFP dimerization prevention (A206K) were introduced. [MGI Ref ID J:157448] [MGI Ref ID J:179273] | ||
| Gene Symbol and Name | Tg(tetO-GCaMP2)12IRyu, transgene insertion 12I, Ron Yu | ||
| Chromosome | UN | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetO-GCaMP2)12iRyu, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Yu R. 2012. Direct Data Submission 2012/01/17 MGI Direct Data Submission :. [MGI Ref ID J:179273]
Additional References
Tg(tetO-GCaMP2)12IRyu relatedHe J; Ma L; Kim S; Nakai J; Yu CR. 2008. Encoding gender and individual information in the mouse vomeronasal organ. Science 320(5875):535-8. [PubMed: 18436787] [MGI Ref ID J:157448]
Ma L; Qiu Q; Gradwohl S; Scott A; Yu EQ; Alexander R; Wiegraebe W; Yu CR. 2012. Distributed representation of chemical features and tunotopic organization of glomeruli in the mouse olfactory bulb. Proc Natl Acad Sci U S A 109(14):5481-6. [PubMed: 22431605] [MGI Ref ID J:182656]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX18
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice from the colony. Mating System Noncarrier x Hemizygote (Female x Male) 02-MAY-12 Hemizygote x Noncarrier (Female x Male) 02-MAY-12
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(tetO-GCAMP2)12IRyu
Price per Pair (US dollars $) Pair Genotype $296.00 Hemizygous for Tg(tetO-GCAMP2)12IRyu x Noncarrier $296.00 Noncarrier x Hemizygous for Tg(tetO-GCAMP2)12IRyu Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(tetO-GCAMP2)12IRyu
Price per Pair (US dollars $) Pair Genotype $384.80 Hemizygous for Tg(tetO-GCAMP2)12IRyu x Noncarrier $384.80 Noncarrier x Hemizygous for Tg(tetO-GCAMP2)12IRyu Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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