Strain Name:

129S-Slc11a2tm2Nca/J

Stock Number:

017789

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use
These Dmt1fl mice possess loxP sites flanking exons 6-8 of the solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2 (Slc11a2) gene and have applications in studies related to anemia, iron homeostasis and micronutrient and drug absorption in the intestine.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-OCT-12
Specieslaboratory mouse
 
Donating Investigator Nancy C. Andrews,   Duke University School of Medicine

Description
The divalent metal transporter DMT1, encoded by Slc11a2, is essential for iron and micronutrient uptake in the gut and other tissues. These mice possess loxP sites on either side of exons 6 through 8 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 through 8 deleted in the cre-expressing tissues.

When bred to a strain with Cre recombinase expression in hippocampal neurons, this mutant mouse strain may be useful in studies of the role of iron in neurodevelopment, neurobiology and neurodegenerative diseases.

Development
A targeting vector containing a floxed NEO-CD(cytidine deaminase) cassette was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 8 of the targeted gene, and another loxP site was inserted upstream of exon 6. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. ES cells that had successfully undergone Cre-mediated recombination and no longer retained the cassette but did retain the loxP-flanked exons 6-8 were injected in C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice, and then backcrossed to 129S6/SvEvTac for more than 10 generations.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
   009104 129S4/SvJaeJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Slc11a2
000172   MK/ReJ
View Strains carrying other alleles of Slc11a2     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Anemia, Hypochromic Microcytic, with Iron Overload 1; AHMIO1   (SLC11A2)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research
Anemia, Iron Homeostasis Defects

Metabolism Research
Hemochromatosis
      iron metabolism defects

Neurobiology Research
Cre-lox System
      loxP-flanked Sequences

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Slc11a2tm2Nca
Allele Name targeted mutation 2, Nancy C Andrews
Allele Type Targeted (Floxed/Frt)
Common Name(s) Slc11a2flox;
Mutation Made By Nancy Andrews,   Duke University School of Medicine
Strain of Origin129S4/SvJae
Gene Symbol and Name Slc11a2, solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Chromosome 15
Gene Common Name(s) DCT1; DMT1; NRAMP2; Nramp2; microcytic anemia; microcytic anemia, viable anaemia; mk; natural resistance-associated macrophage protein 2; van;
Molecular Note A single loxP site was inserted into intron 5 and a loxP flanked cytidine deaminase (CD)- neomycin cassette was inserted into intron 8. Transient cre expression removed the CD-neo cassette. [MGI Ref ID J:98088]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Melt Curve Analysis, Melt Curve Analysis
Slc11a2tm2Nca, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Gunshin H; Fujiwara Y; Custodio AO; Direnzo C; Robine S; Andrews NC. 2005. Slc11a2 is required for intestinal iron absorption and erythropoiesis but dispensable in placenta and liver. J Clin Invest 115(5):1258-1266. [PubMed: 15849611]  [MGI Ref ID J:98088]

Additional References

Slc11a2tm2Nca related

Anderson ER; Taylor M; Xue X; Ramakrishnan SK; Martin A; Xie L; Bredell BX; Gardenghi S; Rivella S; Shah YM. 2013. Intestinal HIF2alpha promotes tissue-iron accumulation in disorders of iron overload with anemia. Proc Natl Acad Sci U S A 110(50):E4922-30. [PubMed: 24282296]  [MGI Ref ID J:205514]

Carlson ES; Magid R; Petryk A; Georgieff MK. 2008. Iron deficiency alters expression of genes implicated in Alzheimer disease pathogenesis. Brain Res 1237:75-83. [PubMed: 18723004]  [MGI Ref ID J:145075]

Carlson ES; Tkac I; Magid R; O'Connor MB; Andrews NC; Schallert T; Gunshin H; Georgieff MK; Petryk A. 2009. Iron is essential for neuron development and memory function in mouse hippocampus. J Nutr 139(4):672-9. [PubMed: 19211831]  [MGI Ref ID J:146816]

Hansen JB; Tonnesen MF; Madsen AN; Hagedorn PH; Friberg J; Grunnet LG; Heller RS; Nielsen AO; Storling J; Baeyens L; Anker-Kitai L; Qvortrup K; Bouwens L; Efrat S; Aalund M; Andrews NC; Billestrup N; Karlsen AE; Holst B; Pociot F; Mandrup-Poulsen T. 2012. Divalent metal transporter 1 regulates iron-mediated ROS and pancreatic beta cell fate in response to cytokines. Cell Metab 16(4):449-61. [PubMed: 23000401]  [MGI Ref ID J:191004]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHeterozygote x Heterozygote         (Female x Male)   10-OCT-12

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
   009104 129S4/SvJaeJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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