Strain Name:

129S-Slc40a1tm2Nca/J

Stock Number:

017790

Order this mouse

Availability:

Repository- Live

Use Restrictions Apply, see Terms of Use
These ferroportinfl mice possess loxP sites flanking exons 6 and 7 of the solute carrier family 40 (iron-regulated transporter), member 1 (Slc40a1) gene and have applications in studies related to type 4 hemochromatosis, anemia, and iron homeostasis.

Description

Strain Information

Type Coisogenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   15-AUG-13
Specieslaboratory mouse
Generation?+N1F3 (10-DEC-13)
Generation Definitions
 
Donating Investigator Nancy C. Andrews,   Duke University School of Medicine

Description
Ferroportin is the only known iron exporter and is essential for iron homeostasis and iron absorption in the intestine. Mutations in the human ferroportin gene, SLC40A1 (solute carrier family 40 (iron-regulated transporter), member 1), can cause type 4 hemochromatosis. These mice possess loxP sites on either side of exons 6 and 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 6 and 7 deleted in the cre-expressing tissues.

When bred to a strain with inducible Cre recombinase expression in the intestine, this mutant mouse strain may be useful in studies of intestinal iron absorption and anemia.

When bred to a strain expressing Cre recombinase in epiblast derived tissues (Meox2tm1(cre)Sor; see Stock No. 003755 for example), this mutant mouse strain may be useful in studies of iron homeostasis.

Development
A targeting vector containing a floxed NEO-CD(cytidine deaminase) cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 6 of the targeted gene, and another loxP site was inserted downstream of exon 7. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected in C57BL/6 blastocysts. The resulting chimeric animals were crossed to 129S6/SvEvTac mice, and then crossed to mice carrying the Tg(EIIa?cre)C5379Lmgd transgene on the 129S6/SvEvTac to remove the floxed selection cassette. Mice that still retained the loxP-flanked exons 6 and 7 were then backcrossed to 129S6/SvEvTac for more than 10 generations.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
   009104 129S4/SvJaeJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Slc40a1
005840   B6.129P2-Slc40a1tm1Dgen/J
View Strains carrying other alleles of Slc40a1     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hemochromatosis, Type 4; HFE4   (SLC40A1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Meox2tm1(cre)Sor/Meox2+ Slc40a1tm2Nca/Slc40a1tm2Nca

        involves: 129S4/SvJae * 129S6/SvEvTac   (conditional)
  • mortality/aging
  • *normal* mortality/aging
    • unlike homozygous null mice, mice with conditional deletion in embryonic tissues that retain expression in the extraembryonic visceral endoderm and in the placenta survive past birth   (MGI Ref ID J:129846)
  • hematopoietic system phenotype
  • abnormal erythrocyte morphology   (MGI Ref ID J:129846)
    • anemia
      • severe iron deficient anemia   (MGI Ref ID J:129846)
    • anisocytosis
    • decreased hemoglobin content
      • at 18 - 22 days of age   (MGI Ref ID J:129846)
    • decreased mean corpuscular hemoglobin
      • hypochromic at P10   (MGI Ref ID J:129846)
      • reduced in both mature erythrocytes and reticulocytes at 18 - 22 days of age   (MGI Ref ID J:129846)
    • decreased mean corpuscular volume
      • at 18 - 22 days of age   (MGI Ref ID J:129846)
    • poikilocytosis
  • increased spleen iron level
    • significant iron accumulation in splenic macrophages at P12   (MGI Ref ID J:129846)
    • at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls   (MGI Ref ID J:129846)
  • reticulocytosis
    • at P10 and at P18 - P22   (MGI Ref ID J:129846)
  • growth/size/body phenotype
  • decreased body size
    • apparent within a few days of birth   (MGI Ref ID J:129846)
    • difference from controls becomes more prominent with age   (MGI Ref ID J:129846)
  • homeostasis/metabolism phenotype
  • abnormal intestinal iron level
    • significant iron accumulation in duodenal enterocytes at P12   (MGI Ref ID J:129846)
  • abnormal liver iron level
    • significant iron accumulation in the Kupffer cells and hepatocytes at P12   (MGI Ref ID J:129846)
    • at P18 - P22 iron levels in the liver are reduced by 1.7-fold compared to controls   (MGI Ref ID J:129846)
  • increased spleen iron level
    • significant iron accumulation in splenic macrophages at P12   (MGI Ref ID J:129846)
    • at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls   (MGI Ref ID J:129846)
  • digestive/alimentary phenotype
  • abnormal intestinal iron level
    • significant iron accumulation in duodenal enterocytes at P12   (MGI Ref ID J:129846)
  • immune system phenotype
  • increased spleen iron level
    • significant iron accumulation in splenic macrophages at P12   (MGI Ref ID J:129846)
    • at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls   (MGI Ref ID J:129846)
  • liver/biliary system phenotype
  • abnormal liver iron level
    • significant iron accumulation in the Kupffer cells and hepatocytes at P12   (MGI Ref ID J:129846)
    • at P18 - P22 iron levels in the liver are reduced by 1.7-fold compared to controls   (MGI Ref ID J:129846)
  • integument phenotype
  • pallor
    • apparent within a few days of birth   (MGI Ref ID J:129846)
    • difference from controls becomes more prominent with age   (MGI Ref ID J:129846)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Hematological Research
Anemia, Iron Deficiency and Transport Defects
Anemia, Iron Homeostasis Defects

Metabolism Research
Hemochromatosis
      iron metabolism defects

Research Tools
Cre-lox System
      loxP-flanked Sequences

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Slc40a1tm2Nca
Allele Name targeted mutation 2, Nancy C Andrews
Allele Type Targeted (Floxed/Frt)
Common Name(s) Ferroportinflox; Fpnflox;
Mutation Made By Nancy Andrews,   Duke University School of Medicine
Strain of Origin129S4/SvJae
Gene Symbol and Name Slc40a1, solute carrier family 40 (iron-regulated transporter), member 1
Chromosome 1
Gene Common Name(s) Dusg; FPN1; HFE4; IREG1; MST079; MTP1; Ol5; Pcm; SLC11A3; Slc11a3; Slc39a1; duodenal-specific gene; ferroportin1; metal transporting protein 1; polycythaemia; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3;
Molecular Note A floxed neo-cassette was inserted in intron 5 and a third loxP site was inserted in intron 7 via homologous recombination. The floxed neo cassette was removed by in vivo cre-mediated recombination leaving exons 6 and 7 flanked by loxP sites. [MGI Ref ID J:129846]

Genotyping

Genotyping Information

Genotyping Protocols

Slc40a1tm2Nca, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Donovan A; Lima CA; Pinkus JL; Pinkus GS; Zon LI; Robine S; Andrews NC. 2005. The iron exporter ferroportin/Slc40a1 is essential for iron homeostasis. Cell Metab 1(3):191-200. [PubMed: 16054062]  [MGI Ref ID J:129846]

Additional References

Slc40a1tm2Nca related

Gabrielsen JS; Gao Y; Simcox JA; Huang J; Thorup D; Jones D; Cooksey RC; Gabrielsen D; Adams TD; Hunt SC; Hopkins PN; Cefalu WT; McClain DA. 2012. Adipocyte iron regulates adiponectin and insulin sensitivity. J Clin Invest 122(10):3529-40. [PubMed: 22996660]  [MGI Ref ID J:191755]

Iacovelli J; Zhao C; Wolkow N; Veldman P; Gollomp K; Ojha P; Lukinova N; King A; Feiner L; Esumi N; Zack DJ; Pierce EA; Vollrath D; Dunaief JL. 2011. Generation of Cre Transgenic Mice with Postnatal RPE-Specific Ocular Expression. Invest Ophthalmol Vis Sci 52(3):1378-83. [PubMed: 21212186]  [MGI Ref ID J:170522]

Mao J; McKean DM; Warrier S; Corbin JG; Niswander L; Zohn IE. 2010. The iron exporter ferroportin 1 is essential for development of the mouse embryo, forebrain patterning and neural tube closure. Development 137(18):3079-88. [PubMed: 20702562]  [MGI Ref ID J:164587]

Schulz K; Kroner A; David S. 2012. Iron efflux from astrocytes plays a role in remyelination. J Neurosci 32(14):4841-7. [PubMed: 22492039]  [MGI Ref ID J:184136]

Zhang Z; Zhang F; An P; Guo X; Shen Y; Tao Y; Wu Q; Zhang Y; Yu Y; Ning B; Nie G; Knutson MD; Anderson GJ; Wang F. 2011. Ferroportin1 deficiency in mouse macrophages impairs iron homeostasis and inflammatory responses. Blood 118(7):1912-22. [PubMed: 21705499]  [MGI Ref ID J:176908]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX18

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice can be bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   15-AUG-13
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $232.00Female or MaleHomozygous for Slc40a1tm2Nca  
Price per Pair (US dollars $)Pair Genotype
$464.00Homozygous for Slc40a1tm2Nca x Homozygous for Slc40a1tm2Nca  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $301.60Female or MaleHomozygous for Slc40a1tm2Nca  
Price per Pair (US dollars $)Pair Genotype
$603.20Homozygous for Slc40a1tm2Nca x Homozygous for Slc40a1tm2Nca  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1500 unique mouse models across a vast array of research areas. Breeding colonies provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. If a Repository strain is not immediately available, then within 2 to 3 business days, you will receive an estimated availability timeframe for your inquiry or order along with various delivery options. Repository strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping. We will note and try to accommodate requests for specific ages of Repository strains but cannot guarantee provision of these strains at specific ages. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, please let us know.

Control Information

  Control
   002448 129S1/SvImJ (approximate)
   009104 129S4/SvJaeJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)