Strain Name:

STOCK Kitltm1.1Sjm/J

Stock Number:

017860

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Availability:

Repository- Live

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An EGFP reporter disrupts expression of the Kitl gene in this targeted mutation strain. EGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow in heterozygotes.

Description

Strain Information

Former Names BKa.129S4-Kitltm1.1Sjm/J    (Changed: 24-FEB-12 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemWild-type x Heterozygote         (Female x Male)   11-APR-13
Mating SystemHeterozygote x Wild-type         (Female x Male)   11-APR-13
Specieslaboratory mouse
GenerationpN1+F2 (18-AUG-14)
Generation Definitions
 
Donating Investigator Sean J Morrison,   UT Southwestern

Description
An Enhanced Green Fluorescent Protein (EGFP) reporter disrupts expression of Kitl (kit ligand; also called stem cell factor, Scf) in this targeted mutation strain. EGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow. Homozygotes die perinatally with severe anemia. Kitl transcripts are nearly undetectable by quantitatve RT-PCR in the livers of newborn homozygotes. Overall cellularity of the newborn liver is reduced fivefold in homozygotes and approximately twofold in heterozygotes. Hematopoietic stem cell numbers are reduced about eightfold in the livers of newborn homozygotes as compared with wildtype and heterozygous controls. Heterozygotes have a diluted coat color and often have a white spot on the abdomen.

Development
An EGFP-polyA-Frt-Neo-Frt cassette was introduced to the second exon of the targeted gene via 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Chimeric mice were bred with C57BL/Ka mice to obtain germline transmission, then bred with C57BL/Ka background mice expressing Flp to excise the neomycin cassette. This strain was backcrossed for 10 generations to C57BL/Ka by the donating laboratory. Upon arrival at The Jackson Laboratory, C57BL/6J was used for rederivation.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Kitl     (15 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Hyperpigmentation, Familial Progressive, 2; FPH2   (KITLG)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Kitltm1.1Sjm/Kitl+

        BKa.129S6-Kitltm1.1Sjm
  • liver/biliary system phenotype
  • liver hypoplasia

Kitltm1.1Sjm/Kitltm1.1Sjm

        BKa.129S6-Kitltm1.1Sjm
  • mortality/aging
  • complete perinatal lethality   (MGI Ref ID J:180431)
  • hematopoietic system phenotype
  • abnormal bone marrow cell physiology
    • bone marrow cells used to repopulate irradiated mice exhibit reduced bone marrow-derived cell production   (MGI Ref ID J:180431)
  • anemia
  • decreased hematopoietic cell number
    • 8-fold in the liver   (MGI Ref ID J:180431)
    • decreased erythrocyte cell number   (MGI Ref ID J:180431)
  • liver/biliary system phenotype
  • liver hypoplasia
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research

Developmental Biology Research
Perinatal Lethality
      Homozygous

Hematological Research
Hematopoietic Defects

Internal/Organ Research
Liver Defects

Research Tools
Fluorescent Proteins
Hematological Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Kitltm1.1Sjm
Allele Name targeted mutation 1.1, Sean J Morrison
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) Scfgfp;
Mutation Made By Sean Morrison,   UT Southwestern
Strain of Origin129S6/SvEvTac
Site of ExpressionEGFP is primarily expressed by perivascular endothelial and stromal cells throughout the bone marrow.
Gene Symbol and Name Kitl, kit ligand
Chromosome 10
Gene Common Name(s) Clo; Con; FPH2; Gb; KL-1; MGF; Mgf; SCF; SF; SHEP7; SLF; Sl; Steel; Steel factor; blaze; blz; cloud gray; contrasted; grizzle-belly; mast cell growth factor; steel; stem cell factor;
Molecular Note A targeting vector was designed to replace exon 2 with EGFP (in frame) and an FRT-flanked neomycin resistance cassette. The neomycin resistance cassette was removed by flp-mediated recombination. qRT-PCR confirmed the absence of transcript expression in the liver. [MGI Ref ID J:180431]

Genotyping

Genotyping Information

Genotyping Protocols

Kitltm1.1Sjm,

SEPARATED MELT



Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ding L; Saunders TL; Enikolopov G; Morrison SJ. 2012. Endothelial and perivascular cells maintain haematopoietic stem cells. Nature 481(7382):457-62. [PubMed: 22281595]  [MGI Ref ID J:180431]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryHeterozygous mice are viable and fertile. Homozygotes die perinatally with severe anemia.
Mating SystemWild-type x Heterozygote         (Female x Male)   11-APR-13
Heterozygote x Wild-type         (Female x Male)   11-APR-13
Diet Information LabDiet® 5K20

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHeterozygous for Kitltm1.1Sjm  
Price per Pair (US dollars $)Pair Genotype
$271.90Heterozygous for Kitltm1.1Sjm x Wild-type for Kitltm1.1Sjm  
$271.90Wild-type for Kitltm1.1Sjm x Heterozygous for Kitltm1.1Sjm  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHeterozygous for Kitltm1.1Sjm  
Price per Pair (US dollars $)Pair Genotype
$353.50Heterozygous for Kitltm1.1Sjm x Wild-type for Kitltm1.1Sjm  
$353.50Wild-type for Kitltm1.1Sjm x Heterozygous for Kitltm1.1Sjm  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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